Changes of plasma C-reactive protein in patients with craniocerebral injury before and after hyperbaric oxygenation: A randomly controlled study BACKGROUND: Plasma inflammatory factor, such as C-reactive prot...Changes of plasma C-reactive protein in patients with craniocerebral injury before and after hyperbaric oxygenation: A randomly controlled study BACKGROUND: Plasma inflammatory factor, such as C-reactive protein, whose content is regarded as a sensitively pathological marked protein and quantitative indexes of central nervous system injury, has been paid more and more attention in clinic. OBJECTIVE: To observe the effects and clinical significance of C-reactive protein in patients with craniocerebral injury after hyperbaric oxygenation. DESIGN: Randomized controlled study. SETTING: Departments of Neurosurgery, Laboratory and Hyperbaric Oxygen, the Second Affiliated Hospital, Medical College of Shantou University. PARTICIPANTS: A total of 60 patients with craniocerebral injury were selected from Department of Neurosurgery, the Second Affiliated Hospital, Medical College of Shantou University from October 2006 to April 2007. There were 37 males and 23 females and the mean age was 26 years. All subjects were certainly diagnosed as history of craniocerebral injury. Patients hospitalized at 24 hours after injury, Glasgow Coma Score ranged from 3 to 12 points, and all patients were certainly diagnosed with CT or MR scanning. Patients and their relatives provided confirmed consent. All the subjects were randomly divided into hyperbaric oxygenation group and control group with 30 in each group. METHODS: Patients in the control group were treated with routinely neurosurgical therapy after hospitalization; however, based the same basic treatment in the control group, patients in the hyperbaric oxygenation group received hyperbaric oxygenation by using iced-wheel four-door 2-cabin air-compression chamber (made in Yantai) from 24 hours to 10 days after operation or injury. After entering the cabin, patients who had a clear consciousness breathed the oxygen by using face mask; contrarily, patients directly breathed the oxygen. Therapeutic project: Expression was increased for about 15–20 minutes, maintained for about 70–80 minutes, and decreased for 20 minutes. Otherwise, pressure was maintained from 0.2 to 0.25 MPa. Hyperbaric oxygenation took an hour for once a day and 10 times were regarded as a course. Venous blood was collected before treatment and on the next day of the first course end. Content of C-reactive protein in plasma was measured with immune turbidimetry in hyperbaric oxygenation group; in addition, content of C-reactive protein in plasma was directly measured with the same method at the corresponding time in the control group. If the content was less or equal to 8 mg/L, it was regarded as normal value. Effects of the two groups were evaluated based on Glasgow Coma Score before and after treatment. MAIN OUTCOME MEASURES: Content of plasma C-reactive protein and Glasgow Coma Score in the two groups before and after treatment. RESULTS: All 60 patients were involved in the final analysis. ① Content of plasma C-reactive protein: The two contents were obviously higher than normal value after craniocerebral injury. There was no significant difference in the two groups before treatment (P 〉 0.05), but both contents were decreased after treatment, and there was significant difference between HBOT group and control group after treatment (t =4.756, P 〈 0.01). In addition, there was significant difference in hyperbaric oxygen therapy group before and after treatment (t =5.236, P 〈 0.01). ② Glasgow Coma Score: There was no significant difference in the two groups before treatment (P 〉 0.05), but scores were increased in both groups after treatment (t =9.92, 2.51, P 〈 0.01, 0.05); on the other hand, therefore, there was significant difference between the two groupsafter treatment (t =9.21, P 〈 0.01). CONCLUSION: Hyperbaric oxygenation can remarkably decrease content of plasma C-reactive protein in patients with craniocerebral injury at the phase of stress.展开更多
Based on the clinical effect of the treatment on 546 Pediatric Shi Ji fever cases, the thesis tries to explore the effectiveness of Traditional Chinese Medicine(TCM) treatment on Pediatric Shi Ji and the relationship ...Based on the clinical effect of the treatment on 546 Pediatric Shi Ji fever cases, the thesis tries to explore the effectiveness of Traditional Chinese Medicine(TCM) treatment on Pediatric Shi Ji and the relationship between Pediatric Shi Ji and fever. The methodology applied is a retrospective analysis on the clinical curative effect of TCM treatment on Shi Ji fever cases in our hospital from January 2008 to December 2012. And the results show that a total effective rate of 96.3% could be guaranteed through either oral Chinese Medicinal Herbs, Chinese Medicine Enema, Massage Therapy, or navel administration with TCM. The thesis holds that Pediatric Shi Ji may cause fever, which could be cured simply by applying TCM treatment (promoting digestion to eliminate stagnation) with less or no use of antibiotics.展开更多
Dear Editor Coronavirus disease 19(COVID-19),caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),remains a pandemic.Cancer patients have a higher risk of poor outcomes for SARS-CoV-2 infection than t...Dear Editor Coronavirus disease 19(COVID-19),caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),remains a pandemic.Cancer patients have a higher risk of poor outcomes for SARS-CoV-2 infection than the general population[1].Vaccines were shown to effectively prevent SARS-CoV-2 infection,severe disease progression,and mortality.Inactivated SARS-CoV-2 vaccines(BBIBPCorV and CoronaVac)have been approved and widely used in China,with the former shown to be more effective than the latter[2].Hence,there is an urgent need to investigate the safety and humoral immune responses of inactivated vaccines in cancer patients.展开更多
Water and sand leakage disasters are likely to occur during construction in water-rich sand layer areas,resulting in ground collapse.The stress-strain action characteristics of discontinuous graded sand under differen...Water and sand leakage disasters are likely to occur during construction in water-rich sand layer areas,resulting in ground collapse.The stress-strain action characteristics of discontinuous graded sand under different internal erosion degrees,and the evolution mechanism of water and sand leakage disasters caused by the internal erosion need to be further explored.Therefore,this paper takes the discontinuous graded sand in a water rich sand layer area in Nanchang City of China as the research object.Considering the influence of different fine particle losses(0,10%,20%and 30%)under the internal erosion of sand,the salt solution method is used to realize the specified loss of fine particles in the internal erosion.The stress-strain behavior after the loss of fine particles due to internal erosion is studied by triaxial shear test.Meanwhile,the physical model test and PFC-CFD method are both used to study the evolution rules of water and sand leakage disaster considered the influence of internal erosion degrees.Results show that:(1)under the same confining pressure,the peak failure strength of sand samples decreases along with the increase of fine particle loss.(2)In the water and sand leakage test of saturated sand,a natural filter channel is formed above the observed soil arch.The greater the loss of fine particles,the steeper and wider the collapse settlement area.(3)The relationship between the cumulative amount of water and sand leakage and time is nonlinear.The total mass loss of sand increases along with the increase of internal erosion degree.(4)After the soil arch is formed around the damaged opening,the sand continues to converge above the soil arch under the action of water flow,resulting in the dense convergence of contact force chains.展开更多
There is a lack of understanding of both the conversion of an unstable glass into a metastable supercooled liquid(MSL) upon heating and the metastability of MSLs. In this study, we investigated the time-and temperatur...There is a lack of understanding of both the conversion of an unstable glass into a metastable supercooled liquid(MSL) upon heating and the metastability of MSLs. In this study, we investigated the time-and temperature-dependent metastability of an MSL using an advanced nano-calorimetric technique. The chosen Au-based metallic glass(Au MG) allowed adequate probing of its MSL in a temperature range between 10 and 70 K above the standard glass transition temperature. We found that the survival time of the MSL state is a quadratic function of temperature. Beyond this duration threshold, the sample undergoes fast crystallization even if it is below the crystallization temperature that is measured using differential scanning calorimetry.Employing transmission electron microscopy, we observed the formation of clusters with a partially ordered lattice structure during relaxation in the Au MG sample fabricated using a nano-calorimeter. The atomic ordering within the clusters was enhanced by increasing time and temperature in the MSL region. Once the as-produced glass entered the MSL stage upon heating followed by a quenching stage at a given rate, the mechanical properties of the quenched glass remained the same regardless of its holding temperature and duration within the MSL region. This work provides insights into the glass-MSL-crystal transformation and offers guidance for designing standard metallic glasses for property characterizations.展开更多
As a common but critical dynamic crossover in glass-forming liquids(GFLs),the discovery of fragile-to-strong(F-S)transition promises a novel route for understanding supercooled liquid and glass transition.The present ...As a common but critical dynamic crossover in glass-forming liquids(GFLs),the discovery of fragile-to-strong(F-S)transition promises a novel route for understanding supercooled liquid and glass transition.The present work,for the first time,successfully realizes the quantitative prediction of the F-S transition in nine metallic glass-forming liquids,by a counter-intuitive approach that focuses on local atomic activation events,rather than relaxation,upon cooling.The dynamic crossover originates from a disorder-to-order transition by self-regulating behavior of atomic position within a cage controlled by finite atomic activation events,due to the appearance of local cooperative motion of nearest neighborhood atoms.Moreover,the dominant role of entropy in this anomaly has been discovered,and the correspondence between the crossover of configuration entropy involved in activation events and the occurrence of F-S transition has been found.Our work implies that the feature of atomic energy fluctuations reflected by atomic activation events has a close linkage to complex dynamic behaviors of disordered systems.展开更多
Dendritic cell-based cancer vaccines(DC vaccines)have been proved efficient and safe in immunotherapy of various cancers,including melanoma,ovarian and prostate cancer.However,the clinical responses were not always sa...Dendritic cell-based cancer vaccines(DC vaccines)have been proved efficient and safe in immunotherapy of various cancers,including melanoma,ovarian and prostate cancer.However,the clinical responses were not always satisfied.Here we proposed a novel strategy to prepare DC vaccines.In the present study,a fusion protein SNU containing a secretin-penetratin(SecPen)peptide,NY-ESO-1 and ubiquitin was designed and expressed.To establish the DC vaccine(DC-SNU),the mouse bone marrowderived DCs(BMDCs)were isolated,pulsed with SNU and maturated with cytokine cocktail.Then peripheral blood mononuclear cells(PBMCs)from C57 BL/6 mice inoculated intraperitoneally with DCSNU were separated and cocultured with MC38/MC38NY-ESO-1 tumor cells or DC vaccines.The results show that SNU was successfully expressed.This strategy made NY-ESO-1 entering cytoplasm of BMDCs more efficiently and degraded mainly by proteasome.As we expected,mature BMDCs expressed higher CD40,CD80 and CD86 than immature BMDCs.Thus,the PBMCs released more IFN-γand TNF-αwhen stimulated with DC-SNU in vitro again.What’s more,the PBMCs induced stronger and specific cytotoxicity towards MC38NY-ESO-1 tumor cells.Given the above,it demonstrated that DC-SNU loaded with SecPen and ubiquitin-fused NY-ESO-1 could elicit stronger and specific T cell immune responses.This strategy can be used as a platform for DC vaccine preparation and applied to various cancers treatment.展开更多
Liquid dynamics plays an essential role in glass formation.Here we observed a distinct change of liquid dynamics in Gd_(55)Co_(20)Al_(25)metallic glass induced by microalloying Si element.In the equilibrium melt,minor...Liquid dynamics plays an essential role in glass formation.Here we observed a distinct change of liquid dynamics in Gd_(55)Co_(20)Al_(25)metallic glass induced by microalloying Si element.In the equilibrium melt,minor Si(0.5 at.%)addition leads to a more fragile liquid behavior and a smaller strength of liquid-liquid transition with the transition strength(F)decreasing from 0.76 to 0.35.However,in the supercooled liquid,Si-doped liquid exhibits a remarkable enhanced fragile-to-strong transition(FST),and the value of FST factor f increases sharply from 1.63 to 3.84,resulting in a stronger liquid behavior and more sluggish crystallization kinetics for Gd_(55)Co_(20)Al_(24.5)Si_(0.5)metallic glass.Moreover,minor Si addition promotes the formation of a crystal-like structure with a size of 1-2 nm.The interactions between the crystal-like structures and other local favored clusters frustrate the further growth of crystal-like phases,thus stabilizes the amorphous structure.As a result,the glass-forming ability(GFA)was largely improved.The critical diameter of Gd_(55)Co_(20)Al_(25)metallic glass increased from 2 to 7 mm with 0.5 at.%Si addition without deterioration of the magnetocaloric effect.This study provides valuable insight for understanding the distinct effect of microalloying on GFA of metallic glasses from the aspect of the evolution of the liquid.展开更多
The authors consider a free interface problem which stems from a gas-solid model in combustion with pattern formation. A third-order, fully nonlinear, self-consistent equation for the flame front is derived. Asymptoti...The authors consider a free interface problem which stems from a gas-solid model in combustion with pattern formation. A third-order, fully nonlinear, self-consistent equation for the flame front is derived. Asymptotic methods reveal that the interface approaches a solution to the Kuramoto-Sivashinsky equation. Numerical results which illustrate the dynamics are presented.展开更多
Recently,evidence has suggested that chronic endometritis(CE)is a crucial factor associated with infertility and failure of assisted reproductive techniques,prompting concern in the reproductive field.Studies have sho...Recently,evidence has suggested that chronic endometritis(CE)is a crucial factor associated with infertility and failure of assisted reproductive techniques,prompting concern in the reproductive field.Studies have shown that persistent infiltered immune cells stimulation result in the disturbance of endometrial immune microenvironment could lead to the infertility of CE patients finally.Conventional treatments are limited because they lack immune regulation,so it is urgent to develop a novel approach to treat CE and promote embryo implantation in patients with CE.Herein,we prepared recombinant humanized type III collagen(rhCol III)with high cell adhesion activity to regulate macrophages and repair the endometrium.In this study,M1 macrophages and M1 macrophages cultured medium and lipopolysaccharide(LPS)co-stimulated inflammatory endometrium stromal cells(ESCs)were established in vitro to mimic CE condition.rhCol III promoted M1 macrophages toward M2 phenotype,improved cell migration,viability and collagen components of inflammatory ESCs.Also,the inflammatory response of inflammatory ESCs was downregulated after rhCol III treatment.Subsequently,LPS was used for CE rat model and a 28-day observation was performed;inflammatory cells’infiltration,endometrium repair,extracellular matrix(ECM)remodeling and pregnancy outcomes were promoted after rhCol III endometrial infusion.In conclusion,rhCol III promoted(i)macrophage polarization toward M2 macrophages,(ii)pro-inflammatory cytokine production and anti-inflammatory cytokine reduction,(iii)ECM remodeling and(iv)fertility restoration.Meanwhile,rhCol III enhanced cell biological functions by interacting with discoidin domain receptors,regulated cell metabolism and reduced the inflammatory response through the inhibition of the NF-κB/YAP signaling pathway.Overall,the results illustrated the potential therapeutic prospects of rhCol III for CE treatment.展开更多
文摘Changes of plasma C-reactive protein in patients with craniocerebral injury before and after hyperbaric oxygenation: A randomly controlled study BACKGROUND: Plasma inflammatory factor, such as C-reactive protein, whose content is regarded as a sensitively pathological marked protein and quantitative indexes of central nervous system injury, has been paid more and more attention in clinic. OBJECTIVE: To observe the effects and clinical significance of C-reactive protein in patients with craniocerebral injury after hyperbaric oxygenation. DESIGN: Randomized controlled study. SETTING: Departments of Neurosurgery, Laboratory and Hyperbaric Oxygen, the Second Affiliated Hospital, Medical College of Shantou University. PARTICIPANTS: A total of 60 patients with craniocerebral injury were selected from Department of Neurosurgery, the Second Affiliated Hospital, Medical College of Shantou University from October 2006 to April 2007. There were 37 males and 23 females and the mean age was 26 years. All subjects were certainly diagnosed as history of craniocerebral injury. Patients hospitalized at 24 hours after injury, Glasgow Coma Score ranged from 3 to 12 points, and all patients were certainly diagnosed with CT or MR scanning. Patients and their relatives provided confirmed consent. All the subjects were randomly divided into hyperbaric oxygenation group and control group with 30 in each group. METHODS: Patients in the control group were treated with routinely neurosurgical therapy after hospitalization; however, based the same basic treatment in the control group, patients in the hyperbaric oxygenation group received hyperbaric oxygenation by using iced-wheel four-door 2-cabin air-compression chamber (made in Yantai) from 24 hours to 10 days after operation or injury. After entering the cabin, patients who had a clear consciousness breathed the oxygen by using face mask; contrarily, patients directly breathed the oxygen. Therapeutic project: Expression was increased for about 15–20 minutes, maintained for about 70–80 minutes, and decreased for 20 minutes. Otherwise, pressure was maintained from 0.2 to 0.25 MPa. Hyperbaric oxygenation took an hour for once a day and 10 times were regarded as a course. Venous blood was collected before treatment and on the next day of the first course end. Content of C-reactive protein in plasma was measured with immune turbidimetry in hyperbaric oxygenation group; in addition, content of C-reactive protein in plasma was directly measured with the same method at the corresponding time in the control group. If the content was less or equal to 8 mg/L, it was regarded as normal value. Effects of the two groups were evaluated based on Glasgow Coma Score before and after treatment. MAIN OUTCOME MEASURES: Content of plasma C-reactive protein and Glasgow Coma Score in the two groups before and after treatment. RESULTS: All 60 patients were involved in the final analysis. ① Content of plasma C-reactive protein: The two contents were obviously higher than normal value after craniocerebral injury. There was no significant difference in the two groups before treatment (P 〉 0.05), but both contents were decreased after treatment, and there was significant difference between HBOT group and control group after treatment (t =4.756, P 〈 0.01). In addition, there was significant difference in hyperbaric oxygen therapy group before and after treatment (t =5.236, P 〈 0.01). ② Glasgow Coma Score: There was no significant difference in the two groups before treatment (P 〉 0.05), but scores were increased in both groups after treatment (t =9.92, 2.51, P 〈 0.01, 0.05); on the other hand, therefore, there was significant difference between the two groupsafter treatment (t =9.21, P 〈 0.01). CONCLUSION: Hyperbaric oxygenation can remarkably decrease content of plasma C-reactive protein in patients with craniocerebral injury at the phase of stress.
文摘Based on the clinical effect of the treatment on 546 Pediatric Shi Ji fever cases, the thesis tries to explore the effectiveness of Traditional Chinese Medicine(TCM) treatment on Pediatric Shi Ji and the relationship between Pediatric Shi Ji and fever. The methodology applied is a retrospective analysis on the clinical curative effect of TCM treatment on Shi Ji fever cases in our hospital from January 2008 to December 2012. And the results show that a total effective rate of 96.3% could be guaranteed through either oral Chinese Medicinal Herbs, Chinese Medicine Enema, Massage Therapy, or navel administration with TCM. The thesis holds that Pediatric Shi Ji may cause fever, which could be cured simply by applying TCM treatment (promoting digestion to eliminate stagnation) with less or no use of antibiotics.
基金the Ethics Committee of the Second Affiliated Hospital of Chongqing Medical University and conformed with the ethical guidelines of the Declaration of Helsinki(Ratification No.133/2022)Written informed consentwas obtained fromall the participants.This study has been registered at ClinicalTrials.gov(NCT05043246).
文摘Dear Editor Coronavirus disease 19(COVID-19),caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),remains a pandemic.Cancer patients have a higher risk of poor outcomes for SARS-CoV-2 infection than the general population[1].Vaccines were shown to effectively prevent SARS-CoV-2 infection,severe disease progression,and mortality.Inactivated SARS-CoV-2 vaccines(BBIBPCorV and CoronaVac)have been approved and widely used in China,with the former shown to be more effective than the latter[2].Hence,there is an urgent need to investigate the safety and humoral immune responses of inactivated vaccines in cancer patients.
基金funded by the Natural Science Foundation of China(No.41962015).
文摘Water and sand leakage disasters are likely to occur during construction in water-rich sand layer areas,resulting in ground collapse.The stress-strain action characteristics of discontinuous graded sand under different internal erosion degrees,and the evolution mechanism of water and sand leakage disasters caused by the internal erosion need to be further explored.Therefore,this paper takes the discontinuous graded sand in a water rich sand layer area in Nanchang City of China as the research object.Considering the influence of different fine particle losses(0,10%,20%and 30%)under the internal erosion of sand,the salt solution method is used to realize the specified loss of fine particles in the internal erosion.The stress-strain behavior after the loss of fine particles due to internal erosion is studied by triaxial shear test.Meanwhile,the physical model test and PFC-CFD method are both used to study the evolution rules of water and sand leakage disaster considered the influence of internal erosion degrees.Results show that:(1)under the same confining pressure,the peak failure strength of sand samples decreases along with the increase of fine particle loss.(2)In the water and sand leakage test of saturated sand,a natural filter channel is formed above the observed soil arch.The greater the loss of fine particles,the steeper and wider the collapse settlement area.(3)The relationship between the cumulative amount of water and sand leakage and time is nonlinear.The total mass loss of sand increases along with the increase of internal erosion degree.(4)After the soil arch is formed around the damaged opening,the sand continues to converge above the soil arch under the action of water flow,resulting in the dense convergence of contact force chains.
基金supported by the Songshan Lake Materials Laboratory (Grant No. 2021SLABFN05)the National Natural Science Foundation of China (Grant Nos. 51971120, 51901139)+2 种基金the Taishan Scholars Program of Shandong Province (Grant No. tsqn201909010)the Key Basic and Applied Research Program of Guangdong Province(Grant No. 2019B030302010)the Sao Paulo Research Foundation–FAPESP (Grant No.#2013/07793-6)。
文摘There is a lack of understanding of both the conversion of an unstable glass into a metastable supercooled liquid(MSL) upon heating and the metastability of MSLs. In this study, we investigated the time-and temperature-dependent metastability of an MSL using an advanced nano-calorimetric technique. The chosen Au-based metallic glass(Au MG) allowed adequate probing of its MSL in a temperature range between 10 and 70 K above the standard glass transition temperature. We found that the survival time of the MSL state is a quadratic function of temperature. Beyond this duration threshold, the sample undergoes fast crystallization even if it is below the crystallization temperature that is measured using differential scanning calorimetry.Employing transmission electron microscopy, we observed the formation of clusters with a partially ordered lattice structure during relaxation in the Au MG sample fabricated using a nano-calorimeter. The atomic ordering within the clusters was enhanced by increasing time and temperature in the MSL region. Once the as-produced glass entered the MSL stage upon heating followed by a quenching stage at a given rate, the mechanical properties of the quenched glass remained the same regardless of its holding temperature and duration within the MSL region. This work provides insights into the glass-MSL-crystal transformation and offers guidance for designing standard metallic glasses for property characterizations.
基金supported by the National Key R&D Program of China (2018YFA0703600)the National Natural Science Foundation of China (51922102, 52001319, 52271158, 92163108, and 52231006)+2 种基金Zhejiang Provincial Natural Science Foundation (LGF22E010002, LZ22A030001, and LR22E010004)the “Pioneer and Leading Goose” R&D Program of Zhejiang (2022C01023)Ningbo Key Scientific and Technological Project (2019B10051)。
基金supported by the National Natural Science Foundation of China(Grant Nos.51901139,U1902221,51971120,and 51971093)the Taishan Scholars Program of Shandong Province(Grant No.tsqn201909010)the Key Basic and Applied Research Program of Guangdong Province(Grant No.2019B030302010)。
文摘As a common but critical dynamic crossover in glass-forming liquids(GFLs),the discovery of fragile-to-strong(F-S)transition promises a novel route for understanding supercooled liquid and glass transition.The present work,for the first time,successfully realizes the quantitative prediction of the F-S transition in nine metallic glass-forming liquids,by a counter-intuitive approach that focuses on local atomic activation events,rather than relaxation,upon cooling.The dynamic crossover originates from a disorder-to-order transition by self-regulating behavior of atomic position within a cage controlled by finite atomic activation events,due to the appearance of local cooperative motion of nearest neighborhood atoms.Moreover,the dominant role of entropy in this anomaly has been discovered,and the correspondence between the crossover of configuration entropy involved in activation events and the occurrence of F-S transition has been found.Our work implies that the feature of atomic energy fluctuations reflected by atomic activation events has a close linkage to complex dynamic behaviors of disordered systems.
基金funded by PDH-SPFDU Joint Research Fund(RHJJ2018-04,China)Shanghai Science and Technology Committee(No.19431903000,China)
文摘Dendritic cell-based cancer vaccines(DC vaccines)have been proved efficient and safe in immunotherapy of various cancers,including melanoma,ovarian and prostate cancer.However,the clinical responses were not always satisfied.Here we proposed a novel strategy to prepare DC vaccines.In the present study,a fusion protein SNU containing a secretin-penetratin(SecPen)peptide,NY-ESO-1 and ubiquitin was designed and expressed.To establish the DC vaccine(DC-SNU),the mouse bone marrowderived DCs(BMDCs)were isolated,pulsed with SNU and maturated with cytokine cocktail.Then peripheral blood mononuclear cells(PBMCs)from C57 BL/6 mice inoculated intraperitoneally with DCSNU were separated and cocultured with MC38/MC38NY-ESO-1 tumor cells or DC vaccines.The results show that SNU was successfully expressed.This strategy made NY-ESO-1 entering cytoplasm of BMDCs more efficiently and degraded mainly by proteasome.As we expected,mature BMDCs expressed higher CD40,CD80 and CD86 than immature BMDCs.Thus,the PBMCs released more IFN-γand TNF-αwhen stimulated with DC-SNU in vitro again.What’s more,the PBMCs induced stronger and specific cytotoxicity towards MC38NY-ESO-1 tumor cells.Given the above,it demonstrated that DC-SNU loaded with SecPen and ubiquitin-fused NY-ESO-1 could elicit stronger and specific T cell immune responses.This strategy can be used as a platform for DC vaccine preparation and applied to various cancers treatment.
基金the National Natural Science Foundation of China(Nos.51631003,51571131,51971061 and 51471050)the National Key Research Program of China(No.2016YFB0300500)。
文摘Liquid dynamics plays an essential role in glass formation.Here we observed a distinct change of liquid dynamics in Gd_(55)Co_(20)Al_(25)metallic glass induced by microalloying Si element.In the equilibrium melt,minor Si(0.5 at.%)addition leads to a more fragile liquid behavior and a smaller strength of liquid-liquid transition with the transition strength(F)decreasing from 0.76 to 0.35.However,in the supercooled liquid,Si-doped liquid exhibits a remarkable enhanced fragile-to-strong transition(FST),and the value of FST factor f increases sharply from 1.63 to 3.84,resulting in a stronger liquid behavior and more sluggish crystallization kinetics for Gd_(55)Co_(20)Al_(24.5)Si_(0.5)metallic glass.Moreover,minor Si addition promotes the formation of a crystal-like structure with a size of 1-2 nm.The interactions between the crystal-like structures and other local favored clusters frustrate the further growth of crystal-like phases,thus stabilizes the amorphous structure.As a result,the glass-forming ability(GFA)was largely improved.The critical diameter of Gd_(55)Co_(20)Al_(25)metallic glass increased from 2 to 7 mm with 0.5 at.%Si addition without deterioration of the magnetocaloric effect.This study provides valuable insight for understanding the distinct effect of microalloying on GFA of metallic glasses from the aspect of the evolution of the liquid.
基金Project supported by a grant from the Fujian Administration of Foreign Expert Affairs,China (No.SZ2011008)
文摘The authors consider a free interface problem which stems from a gas-solid model in combustion with pattern formation. A third-order, fully nonlinear, self-consistent equation for the flame front is derived. Asymptotic methods reveal that the interface approaches a solution to the Kuramoto-Sivashinsky equation. Numerical results which illustrate the dynamics are presented.
基金supported by Scientific and Technological Research Program of Chongqing Municipal Education Commission(Grant No.KJCXZD2020017).
文摘Recently,evidence has suggested that chronic endometritis(CE)is a crucial factor associated with infertility and failure of assisted reproductive techniques,prompting concern in the reproductive field.Studies have shown that persistent infiltered immune cells stimulation result in the disturbance of endometrial immune microenvironment could lead to the infertility of CE patients finally.Conventional treatments are limited because they lack immune regulation,so it is urgent to develop a novel approach to treat CE and promote embryo implantation in patients with CE.Herein,we prepared recombinant humanized type III collagen(rhCol III)with high cell adhesion activity to regulate macrophages and repair the endometrium.In this study,M1 macrophages and M1 macrophages cultured medium and lipopolysaccharide(LPS)co-stimulated inflammatory endometrium stromal cells(ESCs)were established in vitro to mimic CE condition.rhCol III promoted M1 macrophages toward M2 phenotype,improved cell migration,viability and collagen components of inflammatory ESCs.Also,the inflammatory response of inflammatory ESCs was downregulated after rhCol III treatment.Subsequently,LPS was used for CE rat model and a 28-day observation was performed;inflammatory cells’infiltration,endometrium repair,extracellular matrix(ECM)remodeling and pregnancy outcomes were promoted after rhCol III endometrial infusion.In conclusion,rhCol III promoted(i)macrophage polarization toward M2 macrophages,(ii)pro-inflammatory cytokine production and anti-inflammatory cytokine reduction,(iii)ECM remodeling and(iv)fertility restoration.Meanwhile,rhCol III enhanced cell biological functions by interacting with discoidin domain receptors,regulated cell metabolism and reduced the inflammatory response through the inhibition of the NF-κB/YAP signaling pathway.Overall,the results illustrated the potential therapeutic prospects of rhCol III for CE treatment.