High-throughput sorting of two-color fluorescent-labeled zebrafish embryos

The zebrafish embryos were widely employed in genetics,development and drug discovery studies as miniatured animal models.Sorting of two-color fluorescent embryos is often required in large-scale experiments but it is challenging to manually sort with high efficiency.Here,we reported a high-throughput sorting system for two-color fluorescent zebraflsh embryos.The embryos can be automatically loaded from a sample pool and sorted based on the average fluorescent intensity.The two-color fluorescent signals were split into two lines and detected by an area array camera.The system achieves the sorting of 100 embryos in less than 10 min with an accuracy of greater than 95%.

The crosstalk between autophagy and ferroptosis:what can we learn to target drug resistance in cancer?

Autophagy is a conserved intracellular degradation system that plays a dual role in cell death;thus,therapies targeting autophagy in cancer are somewhat controversial.Ferroptosis is a new form of regulated cell death featured with the iron-dependent accumulation of lethal lipid ROS.This pathway is morphologically,biochemically and genetically distinct from other forms of cell death.Accumulating studies have revealed crosstalk between autophagy and ferroptosis at the molecular level.In this review,we summarize the mechanisms of ferroptosis and autophagy,and more importantly,their roles in the drug resistance of cancer.Numerous connections between ferroptosis and autophagy have been revealed,and a strong causal relationship exists wherein one process controls the other and can be utilized as potential therapeutic targets for cancer.The elucidation of when and how to modulate their crosstalk using therapeutic strategies depends on an understanding of the fine-tuned switch between ferroptosis and autophagy,and approaches designed to manipulate the intensity of autophagy might be the key.

Fringe optimization for structured illumination super-resolution microscope with digital micromirror device

Structured illumination microscopy(SIM)is a promising super-resolution technique for imaging subcellular structures and dynamics due to its compatibility with most commonly usedffuorescent labeling methods.Structured illumination can be obtained by either laser interference or projection of fringe patterns.Here,we proposed a fringe projector composed of a compact multiwavelength LEDs module and a digital micromirror device(DMD)which can be directly attached to most commercial invertedffuorescent microscopes and update it into a SIM system.The effects of the period and duty cycle of fringe patterns on the modulation depth of the structured lightfield were studied.With the optimized fringe pattern,1:6×resolution improvement could be obtained with high-end oil objectives.Multicolor imaging and dynamics of subcellular organelles in live cells were also demonstrated.Our method provides a low-cost solution for SIM setup to expand its wide range of applications to most research labs in thefield of life science and medicine.

Influence of Steam and Sulfide on High Temperature Selective Catalytic Reduction

The influences of steam and sulfide on the efficiency of NOx reduction using ammonia(NH_(3))over the nanometer-class V-W/Ti catalyst in conditions of high temperature is experimentally investigated using a steady-flow reactor.The results showed that selective catalytic reduction(SCR)is inhibited by H_(2)O at low temperature,but higher NO conversion efficiency is achieved at high temperature since the reaction of NH_(3) oxidized by O_(2) to NOx is inhibited by H_(2)O.The activity of SCR is promoted by SO_(2) in the temperature range of 200~500℃,the NO conversion efficiency was improved to 98%from 94%by adding SO_(2).SCR would be improved at 350~500℃ when H_(2)O and SO_(2) exist at the same time.Furthermore,the positive influence to the NOx conversion was proved in the presence of H_(2)O and SO_(2) as a result of the European Stationary Cycle test.

正交直线扫描计算机分层成像研究

直线扫描计算机分层成像(CL)在扫描运动方向上的成像分辨率较高,但在垂直于扫描运动方向的成像空间分辨率较低。提出一种正交直线扫描CL方法,该方法分别从两个正交方向对检测对象进行直线CL扫描并重建图像,可实现对检测对象两个正交方向的高分辨率成像。建立了正交直线扫描CL方法的几何模型,分析了扫描运动,搭建了实验系统,图像重建采用同步迭代重建(SIRT)算法。仿真和扫描实验结果表明,正交直线扫描CL方法可行,可用于对平板类零部件的无损检测。

Cancer-associated fibroblasts in breast cancer:Challenges and opportunities

The tumor microenvironment is proposed to contribute substantially to the progression of cancers,including breast cancer.Cancer-associated fibroblasts(CAFs)are the most abundant components of the tumor microenvironment.Studies have revealed that CAFs in breast cancer originate from several types of cells and promote breast cancer malignancy by secreting factors,generating exosomes,releasing nutrients,reshaping the extracellular matrix,and suppressing the function of immune cells.CAFs are also becoming therapeutic targets for breast cancer due to their specific distribution in tumors and their unique biomarkers.Agents interrupting the effect of CAFs on surrounding cells have been developed and applied in clinical trials.Here,we reviewed studies examining the heterogeneity of CAFs in breast cancer and expression patterns of CAF markers in different subtypes of breast cancer.We hope that summarizing CAFrelated studies from a historical perspective will help to accelerate the development of CAF-targeted therapeutic strategies for breast cancer.

植物蛋白质SUMO化修饰体外高效检测系统

蛋白质SUMO化修饰是一种调控蛋白命运的关键修饰方式,广泛参与植物生长发育及逆境胁迫响应。SUMO化修饰过程主要由激活酶(E1)-结合酶(E2)-连接酶(E3)组成的级联酶促反应催化,其关键酶组分将SUMO分子缀合至底物蛋白的赖氨酸残基,形成共价异肽键以完成SUMO化修饰过程。该文报道了1种植物蛋白质SUMO化修饰体外高效检测系统,通过在大肠杆菌(Escherichia coli)中构建拟南芥(Arabidopsis thaliana) SUMO化修饰的关键通路实现对底物蛋白的SUMO化修饰,结果可通过免疫印迹进行检测。该系统可以简化植物蛋白质SUMO化修饰的检测流程,为植物细胞SUMO化修饰的功能研究提供了有力工具。

Study on sedimentation distribution based on cooperative analysis of geologic-geophysical in less well area: A case study of Suweiyi sandstone in the DB area, Kuqa Depression in Tarim Basin

The Kuqa Depression in Tarim Basin develops fan deltaic,braided river deltaic,and lacustrine sedi-mentation,espeially extensive braided river deltaic sedimentation,in the Cretaceous-Paleogene Sys-tems.However,fine sedimentary pattern of the Kuqa Depression and North Tarim Uplift belt since the Middle Cenozoic still needs to be studied.As a combination zone of the Kuqa Depression and North Tarim Uplift,the sedimentary characteristics of the DB area in the southern margin of the east of the Kuqa depression have been paid more attention.To better understand the sedimentary framework in the Kuqa Depression and North Tarim Uplift in the Mesozoic and Cenozoic,through joint geologic-geophysical study,sedime ntation of sandstone at the bottom of the Paleogene Suweiyi Formation in the DB area and its peripheral area is investigated.Sedimentary facies and sandstone distribution in the area are identified through core observation,component analysis,logging interpretation and seismic inversion.Based on seismic facies analysis,sedimentary facies distribution in the area is delineated.Results show that sandstone at the bottom of the Paleogene Suweiyi Fommation in the DB area and its peripheral area is developed in shore shallow lacustrine beach-bar facies.The beach-bar arenaceous sediments are mainly distnibuted in the southern DB area and the shallow lacustrine mudstone is developed in northern DB area,showing obvious north south di ferentiation charactenistics.

Palaeoclimate of the Paleogene Dongying Formation in Bozhong Sag of Bohai Bay Basin

It was showed that the sedimentary environment of lacustrine basins is more susceptible to basin size than that of marine basins,leading to frequent changes in sedimentary environment and large differences in sediment supply and types.Based on the comprehensive analysis of clay minerals,spore pollen,major elements and trace elements,the sedimentary environment of Dongying Formation and its evolution in Bozhong Sag were studied.The results showed that during the sedimentary period of Dongying Formation,the study area was in a subtropical warm and humid climate as a whole.In addition,the vertical distribution of spore pollen and algae was divided into four zones and each one represents different sedimentary environments.Combination zone I is characterized by a large number of spore pollen and algae,including juglanspollenites verus,ulmipollemites minor,abietineaepollenites and pinuspollenites,taxodiaceaepollenites hiatus,leiosphaeridia and granodiscus,and it indicates a relatively hot and dry climatic;Combination zone II mainly contains juglanspollenites verus,ulmipollemites minor,abietineaepollenites and pinuspollenites,pediastrum and leiosphaeridia.Compared with I,the number of spore pollen and algae decreases,but the number of pediastrum increases a lot,which represents warm and humid climate.The combination zone III is dominated by abietineaepollenites and pinuspollenites and chaetosphaeridium,and the species and quantity decrease significantly.It indicates a relatively hot and humid climate at that time.Combination zone IV contains a small amount of spore pollen and algae,and it means the climate was warm and humid at that period.The frequent changes of sedimentary environment in lacustrine basins are influenceded by regional tectonic environment and basin scale.Using S/A and E-T parameters,the paleoclimate in the study area was delineated and 10 sedimentary environment interfaces were identified,which further illustrated the characteristics of frequent fluctuations of sedimentary environment in lacustrine basins.