The pots discovered in Inner Mongolia and Hebei Province are huge with a maximum diameter of 9 m and a depth of 3.5 m, singular in shape,caused by weathering and wind erosion and have certain direction. They are a kin...The pots discovered in Inner Mongolia and Hebei Province are huge with a maximum diameter of 9 m and a depth of 3.5 m, singular in shape,caused by weathering and wind erosion and have certain direction. They are a kind of typical climatic geomorphology. This展开更多
Purpose: To investigate the effect of early enteral nutrition on outcomes of trauma patients in the intensive care unit (ICU).Methods: Clinical data of trauma patients in the ICU of Daping Hospital, China from January...Purpose: To investigate the effect of early enteral nutrition on outcomes of trauma patients in the intensive care unit (ICU).Methods: Clinical data of trauma patients in the ICU of Daping Hospital, China from January 2012 to December 2017 was retrospectively analyzed, including patient age, gender, injury mechanism, injury severity score (ISS), nutritional treatment, postoperative complications (wound infection, abdominal abscess, anastomotic rupture, pneumonia), mortality, and adverse events (nausea, vomiting, abdominal distention). Only adult trauma patients who developed bloodstream infection after surgery for damage control were included. Patients were divided into early enteral nutrition group (<48 h) and delayed enteral nutrition group (control group, >48 h). Data of all trauma patients were collected by the same investigator. Data were expressed as frequency (percentage), mean ± standard deviation (normal distribution), or median (Q1, Q3) (non-normal distribution) and analyzed by Chi-square test, Student’st-test, or rank-sum test accordingly. Multiple logistic regression analysis was further adopted to investigate the significant variables with enteral nutrition.Results: Altogether 876 patients were assessed and 110 were eligible for this study, including 93 males and 17 females, with the mean age of (50.0 ± 15.4) years. Traffic accidents (46 cases, 41.8%) and fall from height (31 cases, 28.2%) were the dominant injury mechanism. There were 68 cases in the early enteral nutrition group and 42 cases in the control group. Comparison of general variables between early enteral nutrition group and control group revealed significant difference regarding surgeries of enterectomy (1.5% vs. 19.0%,p = 0.01), ileum/transverse colon/sigmoid colostomy (4.4% vs. 16.3%,p = 0.01) and operation time (h) (3.2 (1.9, 6.1) vs. 4.2 (1.8, 8.8),p = 0.02). Other variables like ISS (p = 0.31), acute physiology and chronic health evaluation≥20 (p = 0.79), etc. had no obvious difference. Chi-square test showed a much better result in early enteral nutrition group than in control group regarding morality (0 vs. 11.9%,p = 0.03), length of hospital stay (days) (76.8 ± 41.4vs. 81.4 ± 44.7,p = 0.01) and wound infection (10.3% vs. 26.2%,p = 0.03). Logistic regression analysis showed that the incidence of wound infection was related to the duration required to achieve the enteral nutrition standard (OR = 1.095,p = 0.002). Seventy-six patients (69.1%) achieved the nutritional goal within a week and 105 patients (95.5%) in the end. Trauma patients unable to reach the enteral nutrition target within one week were often combined with abdominal infection, peritonitis, bowel resection, intestinal necrosis, intestinal fistula, or septic shock.Conclusion: Early enteral nutrition for trauma patients in the ICU is correlated with less wound infection, lower mortality, and shorter hospital stay.展开更多
Phosphorylation affects ubiquitination, stability, and activity of transcriptional factors, thus regulating various cellular functions. E2F transcriptional factor I （E2F1） regulates paternally expressed imprinted ge...Phosphorylation affects ubiquitination, stability, and activity of transcriptional factors, thus regulating various cellular functions. E2F transcriptional factor I （E2F1） regulates paternally expressed imprinted gene 10 （Peg10） expression, thereby promoting cell proliferation. However, the effect of E2FZ stability on PeglO expression and the molecular regulation of E2FZ stability by its phos- phorylation have not been well demonstrated. Here, we describe a new pathway in which phosphorylation of E2F1 by GSK3p increases E2FZ association with the deubiquitinating enzyme, ubiquitin-specific protease 11 （USPll）, which removes K63-1inked ubiquitin chains thereby preventing E2FZ degradation in the nuclei. Downregulation of USPlZ increases E2FZ ubiquitination and reduces E2F1 stability and protein levels, thereby decreasing PeglO mRNA levels. Physiologically, USPll depletion suppresses cell proliferation and wound healing in lung epithelial cells, and these effects are reversed by E2F1 and PEGIO overexpression. Thus, our study reveals a new molecular model that phosphorylation promotes substrate stability through increasing its associ- ation with a deubiquitinating enzyme. The data suggest that GSK3p and USPll act in concert to modulate E2FZ abundance and PEGIO expression in lung epithelial celts to affect cell wound healing. This study provides new therapeutic targets to lessen lung injury by improving lung epithelial cell repair and remodeling after injury.展开更多
文摘The pots discovered in Inner Mongolia and Hebei Province are huge with a maximum diameter of 9 m and a depth of 3.5 m, singular in shape,caused by weathering and wind erosion and have certain direction. They are a kind of typical climatic geomorphology. This
基金This study was supported by the Healthcare Technology Promotion Project of Chongqing(2018jstg014),China.
文摘Purpose: To investigate the effect of early enteral nutrition on outcomes of trauma patients in the intensive care unit (ICU).Methods: Clinical data of trauma patients in the ICU of Daping Hospital, China from January 2012 to December 2017 was retrospectively analyzed, including patient age, gender, injury mechanism, injury severity score (ISS), nutritional treatment, postoperative complications (wound infection, abdominal abscess, anastomotic rupture, pneumonia), mortality, and adverse events (nausea, vomiting, abdominal distention). Only adult trauma patients who developed bloodstream infection after surgery for damage control were included. Patients were divided into early enteral nutrition group (<48 h) and delayed enteral nutrition group (control group, >48 h). Data of all trauma patients were collected by the same investigator. Data were expressed as frequency (percentage), mean ± standard deviation (normal distribution), or median (Q1, Q3) (non-normal distribution) and analyzed by Chi-square test, Student’st-test, or rank-sum test accordingly. Multiple logistic regression analysis was further adopted to investigate the significant variables with enteral nutrition.Results: Altogether 876 patients were assessed and 110 were eligible for this study, including 93 males and 17 females, with the mean age of (50.0 ± 15.4) years. Traffic accidents (46 cases, 41.8%) and fall from height (31 cases, 28.2%) were the dominant injury mechanism. There were 68 cases in the early enteral nutrition group and 42 cases in the control group. Comparison of general variables between early enteral nutrition group and control group revealed significant difference regarding surgeries of enterectomy (1.5% vs. 19.0%,p = 0.01), ileum/transverse colon/sigmoid colostomy (4.4% vs. 16.3%,p = 0.01) and operation time (h) (3.2 (1.9, 6.1) vs. 4.2 (1.8, 8.8),p = 0.02). Other variables like ISS (p = 0.31), acute physiology and chronic health evaluation≥20 (p = 0.79), etc. had no obvious difference. Chi-square test showed a much better result in early enteral nutrition group than in control group regarding morality (0 vs. 11.9%,p = 0.03), length of hospital stay (days) (76.8 ± 41.4vs. 81.4 ± 44.7,p = 0.01) and wound infection (10.3% vs. 26.2%,p = 0.03). Logistic regression analysis showed that the incidence of wound infection was related to the duration required to achieve the enteral nutrition standard (OR = 1.095,p = 0.002). Seventy-six patients (69.1%) achieved the nutritional goal within a week and 105 patients (95.5%) in the end. Trauma patients unable to reach the enteral nutrition target within one week were often combined with abdominal infection, peritonitis, bowel resection, intestinal necrosis, intestinal fistula, or septic shock.Conclusion: Early enteral nutrition for trauma patients in the ICU is correlated with less wound infection, lower mortality, and shorter hospital stay.
文摘Phosphorylation affects ubiquitination, stability, and activity of transcriptional factors, thus regulating various cellular functions. E2F transcriptional factor I （E2F1） regulates paternally expressed imprinted gene 10 （Peg10） expression, thereby promoting cell proliferation. However, the effect of E2FZ stability on PeglO expression and the molecular regulation of E2FZ stability by its phos- phorylation have not been well demonstrated. Here, we describe a new pathway in which phosphorylation of E2F1 by GSK3p increases E2FZ association with the deubiquitinating enzyme, ubiquitin-specific protease 11 （USPll）, which removes K63-1inked ubiquitin chains thereby preventing E2FZ degradation in the nuclei. Downregulation of USPlZ increases E2FZ ubiquitination and reduces E2F1 stability and protein levels, thereby decreasing PeglO mRNA levels. Physiologically, USPll depletion suppresses cell proliferation and wound healing in lung epithelial cells, and these effects are reversed by E2F1 and PEGIO overexpression. Thus, our study reveals a new molecular model that phosphorylation promotes substrate stability through increasing its associ- ation with a deubiquitinating enzyme. The data suggest that GSK3p and USPll act in concert to modulate E2FZ abundance and PEGIO expression in lung epithelial celts to affect cell wound healing. This study provides new therapeutic targets to lessen lung injury by improving lung epithelial cell repair and remodeling after injury.
