Background:Numerous long RNAs were detected in extracellular vesicles(EVs),some of which were related with the tissue origins and immune cell types.This study examined the molecular basis of different traditional Chin...Background:Numerous long RNAs were detected in extracellular vesicles(EVs),some of which were related with the tissue origins and immune cell types.This study examined the molecular basis of different traditional Chinese medicine syndrome diagnoses(also called syndrome differentiation)in pancreatic ductal adenocarcinoma.Methods:128 pancreatic ductal adenocarcinoma patients with different syndrome diagnoses were retrospectively reviewed in this study.Long RNA sequencing was conducted to analyze the EV long RNA profile of plasma samples.Differentially regulated EV long RNAs were annotated and assessed for Gene Ontology pathway enrichment using DAVID.The online program xCell were used to perform the cell-type enrichment analysis.Results:An average of 15,000 annotated genes,mainly including messenger RNAs,were stably detected per sample.Different syndrome diagnoses exhibited unique EV mRNA expression profiles and therefore different enriched pathways.Gene Set Enrichment Analysis discovered transforming growth factor-βand kirsten rat sarcoma viral oncogene homolog signaling activation as the hallmarks of cancer with Shi-Re syndrome.Cell-type enrichment analysis also revealed a varied inflammation/immune cell type distribution among patients with or without Shi-Re diagnosis.Mast cells,platelets and Tregs were significantly enriched but basophils,common lymphoid progenitors,dendritic cells,and conventional dendritic cells were decreased in patients with Shi-Re diagnosis compared with patients without Shi-Re diagnosis.Conclusion:We identified the hallmarks of cancer with different syndrome diagnoses based on plasma EV long RNA sequencing.In particular,transforming growth factor-βand kirsten rat sarcoma viral oncogene homolog signaling activation were the hallmarks of Shi-Re syndrome,which contribute to shape an inflammatory/immune-suppressive tumor microenvironment in pancreatic ductal adenocarcinoma.展开更多
Body misperception plays an important role in the development of weight and dietary disorders among children and adolescents.A school-based health promotion program(2014-2015)was conducted to promote the school health...Body misperception plays an important role in the development of weight and dietary disorders among children and adolescents.A school-based health promotion program(2014-2015)was conducted to promote the school health education and improve the teenagers'physical health among Chinese children and adolescents.Based on this program,we intended to examine weight status and weight misperception among Chinese children and adolescents and to explore the relationship between weight misperception and lifestyle behaviors.A total of 10708 Chinese children and adolescents in 3rd and 7th grade from Shandong and Qinghai province participated in the program.The participants,dietary and activity patterns were clustered by latent class analysis(LCA).Logistic regression analysis was undertaken to explore the relationship between weight perception and demographic factors or dietary and activity patterns.Given the gender-specific difference of children and adolescents,analyses were separately conducted among boys and girls.The total prevalence of weight misperception was 44.50%.Boys,especially those in higher grade and living in wealthier district,were more likely to misperceive body weight.Girls were more likely to overestimate their weight(26.10%)while boys tended to underestimate the weight(28.32%).Three latent dietary and activity patterns including obesogenic pattern,malnourished pattern and healthy pattern were derived.The participants who had weight misperception were more likely to choose unhealthy dietary and exercise activities.The high prevalence of weight misperception was closely related to the unhealthy weight pattern and unhealthy dietary or exercise patterns.Our research found that most children and adolescents failed to perceive their weight correctly and boys tended to underestimate their weight while girls were subjected to overestimation.So,comprehensive intervention programs should focus on improving self-weight awareness,and appropriate guidance should be made to lead the adolescents to more healthy weight pattern.展开更多
基金supported by the National Natural Science Foundation of China(82230122,82025035,82104612)Shanghai Municipal Health Commission program(ZY(2021-2023)-0205-02)the Science Foundation for Young Scholars of Zhongshan Hospital,Fudan University(grant No.2021ZSQN70).
文摘Background:Numerous long RNAs were detected in extracellular vesicles(EVs),some of which were related with the tissue origins and immune cell types.This study examined the molecular basis of different traditional Chinese medicine syndrome diagnoses(also called syndrome differentiation)in pancreatic ductal adenocarcinoma.Methods:128 pancreatic ductal adenocarcinoma patients with different syndrome diagnoses were retrospectively reviewed in this study.Long RNA sequencing was conducted to analyze the EV long RNA profile of plasma samples.Differentially regulated EV long RNAs were annotated and assessed for Gene Ontology pathway enrichment using DAVID.The online program xCell were used to perform the cell-type enrichment analysis.Results:An average of 15,000 annotated genes,mainly including messenger RNAs,were stably detected per sample.Different syndrome diagnoses exhibited unique EV mRNA expression profiles and therefore different enriched pathways.Gene Set Enrichment Analysis discovered transforming growth factor-βand kirsten rat sarcoma viral oncogene homolog signaling activation as the hallmarks of cancer with Shi-Re syndrome.Cell-type enrichment analysis also revealed a varied inflammation/immune cell type distribution among patients with or without Shi-Re diagnosis.Mast cells,platelets and Tregs were significantly enriched but basophils,common lymphoid progenitors,dendritic cells,and conventional dendritic cells were decreased in patients with Shi-Re diagnosis compared with patients without Shi-Re diagnosis.Conclusion:We identified the hallmarks of cancer with different syndrome diagnoses based on plasma EV long RNA sequencing.In particular,transforming growth factor-βand kirsten rat sarcoma viral oncogene homolog signaling activation were the hallmarks of Shi-Re syndrome,which contribute to shape an inflammatory/immune-suppressive tumor microenvironment in pancreatic ductal adenocarcinoma.
基金This study was supported by grants from the National Natural Science Foundation of China(No.81573262)the Fundamental Research Funds for the Central Universities,HUST(No.2016YXZD042).
文摘Body misperception plays an important role in the development of weight and dietary disorders among children and adolescents.A school-based health promotion program(2014-2015)was conducted to promote the school health education and improve the teenagers'physical health among Chinese children and adolescents.Based on this program,we intended to examine weight status and weight misperception among Chinese children and adolescents and to explore the relationship between weight misperception and lifestyle behaviors.A total of 10708 Chinese children and adolescents in 3rd and 7th grade from Shandong and Qinghai province participated in the program.The participants,dietary and activity patterns were clustered by latent class analysis(LCA).Logistic regression analysis was undertaken to explore the relationship between weight perception and demographic factors or dietary and activity patterns.Given the gender-specific difference of children and adolescents,analyses were separately conducted among boys and girls.The total prevalence of weight misperception was 44.50%.Boys,especially those in higher grade and living in wealthier district,were more likely to misperceive body weight.Girls were more likely to overestimate their weight(26.10%)while boys tended to underestimate the weight(28.32%).Three latent dietary and activity patterns including obesogenic pattern,malnourished pattern and healthy pattern were derived.The participants who had weight misperception were more likely to choose unhealthy dietary and exercise activities.The high prevalence of weight misperception was closely related to the unhealthy weight pattern and unhealthy dietary or exercise patterns.Our research found that most children and adolescents failed to perceive their weight correctly and boys tended to underestimate their weight while girls were subjected to overestimation.So,comprehensive intervention programs should focus on improving self-weight awareness,and appropriate guidance should be made to lead the adolescents to more healthy weight pattern.
