Microbial constituents naturally inhabiting the gastrointestinal tract may influence the homeostasis of the gut environment.The presence or overabundance of some bacterial taxa has been reported to be associated with ...Microbial constituents naturally inhabiting the gastrointestinal tract may influence the homeostasis of the gut environment.The presence or overabundance of some bacterial taxa has been reported to be associated with complex diseases,and the metabolites of certain bacteria may contribute to diverse disorders by influencing signaling pathways.Therefore,the study of gut microbial population has emerged as a crucial field and a new potential area of clinical significance. Advances in the methods of microbiota analysis have shed light upon the details including species diversity,microfloral activities as well as the entire gut microbiota.Nevertheless,comprehensive reviews on this subject are still limited.For elucidating the appropriate selection strategy of the methods to address a particular research question,we comprehensively reviewed the continuously improving technologies,classical to newly developed,and dissected their relative advantages and drawbacks.In addition,aiming at the rapidly advancing next-generation sequencing,we enumerated the improvements in mainstream platforms and made the horizontal and vertical comparison among them.Additionally,we demonstrated the four main-omics methods,which may provide further mechanistic insights into the role of microbiota,to propel phylotyping analysis to functional analysis.展开更多
There has been substantial interest in designing and fabricating electrochemical non-enzyme sensors for glucose detection with a focus on the nanocomposites used between metal oxide and the two-dimensional material.In...There has been substantial interest in designing and fabricating electrochemical non-enzyme sensors for glucose detection with a focus on the nanocomposites used between metal oxide and the two-dimensional material.In this work,Co_(3)O_(4)/reduced graphene oxide(rGO)nanocomposite was obtained and used as a modified electrochemical electrode for the detection of trace glucose.According to the results,the electrocatalytic performances of Co_(3)O_(4)nanoparticles for the oxidation of glucose were substantially enhanced with the addition of a small amount of r GO.Specifically,the Co_(3)O_(4)/r GO modified electrode exhibited a sensitivity of 82μA·mmol^(-1)·cm^(-2),a detection limit of 50μmol·L^(-1)(signal noise ratio(S/N)=3),and a fast response time of about 1 s under optimal conditions.The enhanced performances of the Co_(3)O_(4)/r GO modified electrode are attributable to the high absorption oxygen(O_(Ads))and synergistic effect between Co_(3)O_(4)and r GO.The results indicate that Co_(3)O_(4)/r GO nanocomposite is a promising candidate for being used as the active material in real-world electrochemical biosensors.展开更多
文摘Microbial constituents naturally inhabiting the gastrointestinal tract may influence the homeostasis of the gut environment.The presence or overabundance of some bacterial taxa has been reported to be associated with complex diseases,and the metabolites of certain bacteria may contribute to diverse disorders by influencing signaling pathways.Therefore,the study of gut microbial population has emerged as a crucial field and a new potential area of clinical significance. Advances in the methods of microbiota analysis have shed light upon the details including species diversity,microfloral activities as well as the entire gut microbiota.Nevertheless,comprehensive reviews on this subject are still limited.For elucidating the appropriate selection strategy of the methods to address a particular research question,we comprehensively reviewed the continuously improving technologies,classical to newly developed,and dissected their relative advantages and drawbacks.In addition,aiming at the rapidly advancing next-generation sequencing,we enumerated the improvements in mainstream platforms and made the horizontal and vertical comparison among them.Additionally,we demonstrated the four main-omics methods,which may provide further mechanistic insights into the role of microbiota,to propel phylotyping analysis to functional analysis.
基金financially supported by the National Research Foundation of Korea(NRF)grant funded by the Korean government(MSIT)(Nos.2021H1D3A2A01100019 and 2022R1F1074441)supported by the Brain Pool Program funded by the Ministry of Science and ICT through the National Research Foundation of Korea(No.2021H1D3A2A01100019)。
文摘There has been substantial interest in designing and fabricating electrochemical non-enzyme sensors for glucose detection with a focus on the nanocomposites used between metal oxide and the two-dimensional material.In this work,Co_(3)O_(4)/reduced graphene oxide(rGO)nanocomposite was obtained and used as a modified electrochemical electrode for the detection of trace glucose.According to the results,the electrocatalytic performances of Co_(3)O_(4)nanoparticles for the oxidation of glucose were substantially enhanced with the addition of a small amount of r GO.Specifically,the Co_(3)O_(4)/r GO modified electrode exhibited a sensitivity of 82μA·mmol^(-1)·cm^(-2),a detection limit of 50μmol·L^(-1)(signal noise ratio(S/N)=3),and a fast response time of about 1 s under optimal conditions.The enhanced performances of the Co_(3)O_(4)/r GO modified electrode are attributable to the high absorption oxygen(O_(Ads))and synergistic effect between Co_(3)O_(4)and r GO.The results indicate that Co_(3)O_(4)/r GO nanocomposite is a promising candidate for being used as the active material in real-world electrochemical biosensors.
