福清市农村居民对新型冠状病毒疫情及预防认知的调查研究

目的了解福清市农村居民对新型冠状病毒疫情及自我防护的认知情况。方法本研究为横断面研究,采用自行设计的新型冠状病毒疫情及自我防护认知问卷,2020年5—6月共调查福清市农村1426名18岁以上常住居民。采用二元logistic回归模型鉴别新型冠状病毒疫情及其预防不同认知水平居民的主要社会人口学特征。结果福清市农村居民对新型冠状病毒疫情认知的平均得分和中位数得分分别为5.53分和6分;对新型冠状病毒疫情自我保护知识的平均得分和中位数得分分别为10.34分和11分。二元Logistic回归模型显示,年长人群(Adjusted OR 45~59=2.26,95%CI=1.20~4.27;Adjusted OR 60~69=3.13,95%CI=1.63~5.98;Adjusted OR≥70=4.68,95%CI=2.35~9.33)对新型冠状病毒疫情的了解更为缺乏;受教育程度、家庭平均年收入较高的人群对新型冠状病毒疫情的认知水平和相关自我防护知识水平均较高(P<0.05);单身、离异或丧偶人群(Adjusted OR=1.67,95%CI=1.13~2.46)更缺乏对新型冠状病毒疫情自我保护的知识。结论研究表明,福清市农村居民对新型冠状病毒疫情认知水平一般,但相关自我保护知识水平较高。研究建议在面对类似新冠这类重大疫情时,对居民实施相关自我防护知识的宣传教育或更为重要,尤其要加强对无配偶、年龄较大、受教育水平和/或收入较低的重点人群进行自我防范措施的宣传教育。

Identification of Suitable Reference Genes for qRT-PCR Normalization in Tilia miqueliana Maxim

Quantitative real-time polymerase chain reaction(qRT-PCR)is a rapid and effective approach toward detecting the expression patterns of target genes.The selection of a stable reference gene under specific test condition is essential for expressing levels of target genes accurately.Tilia miqueliana,considered endangered,is a prominent native ornamental and honey tree in East China.No study has evaluated the optimal endogenous reference gene for qRT-PCR analysis in T.miqueliana systematically.In this study,fifteen commonly used reference genes were selected as candidate genes,and the stabilities of their expressions were assessed using four algorithms(GeNorm,NormFiner,BestKeeper,and DeltaCt)in nine experimental datasets.The final integrated evaluation was performed using a comprehensive analysis algorithm(RefFinder).Finally,a target MYB transcription factor gene(TmMYB)was used to verify the accuracy of the candidate reference genes.The results showed that PP2αwas the most stable in tissue set,while RPS13 and SAMCD were optimal for heat and cold stress,respectively.Under waterlogging stress,PP2αand TUB were the most stable genes in the leaves and roots,respectively.EF1αand PP2αwere optimal for drying stress in leaf and root tissues.TUB and ACT7 were the most stable genes in the leaf and root tissues under salt stress.This is the first systematic evaluation of candidate reference genes in T.miqueliana,and it will benefit future studies on expression and functional analysis of target genes in T.miqueliana.

Inflammation as a Mediator of Microbiome Dysbiosis-Associated DNA Methylation Changes in Gastric Premalignant Lesions

Evidence for the influence of chronic inflammation induced by microbial dysbiosis on aberrant DNA methylation supports a plausible connexion between disordered microbiota and precancerous lesions of gastric cancer(PLGC).Here,a comprehensive study including multi-omics data was performed to estimate the relationships amongst the gastric microbiome,inflammatory proteins and DNA methylation alterations and their roles in PLGC development.The results demonstrated that gastric dysbacteriosis increased the risk of PLGC and DNA methylation alterations in related tumour suppressor genes.Seven inflammatory biomarkers were identified for antrum and corpus tissues,respectively,amongst which the expression levels of several biomarkers were significantly correlated with the microbial dysbiosis index(MDI)and methylation status of specific tumour suppressor genes.Notably,mediation analysis revealed that microbial dysbiosis partially contributed to DNA methylation changes in the stomach via the inflammatory cytokines C-C motif chemokine 20(CCL20)and tumour necrosis factor receptor superfamily member 9(TNFRSF9).Overall,these results may provide new insights into the mechanisms that might link the gastric microbiome to PLGC.