Indoor localization has gained much attention over several decades due to enormous applications. However, the accuracy of indoor localization is hard to improve because the signal propagation has small scale effects w...Indoor localization has gained much attention over several decades due to enormous applications. However, the accuracy of indoor localization is hard to improve because the signal propagation has small scale effects which leads to inaccurate measurements. In this paper, we propose an efficient learning approach that combines grid search based kernel support vector machine and principle component analysis. The proposed approach applies principle component analysis to reduce high dimensional measurements. Then we design a grid search algorithm to optimize the parameters of kernel support vector machine in order to improve the localization accuracy. Experimental results indicate that the proposed approach reduces the localization error and improves the computational efficiency comparing with K-nearest neighbor, Back Propagation Neural Network and Support Vector Machine based methods.展开更多
Radio propagation in dense and super dense wireless networks as well as indoor-to-outdoor picocell networks can have multiple line-of-sight or multiple specular components. The performance of a dual-hop decode-and-for...Radio propagation in dense and super dense wireless networks as well as indoor-to-outdoor picocell networks can have multiple line-of-sight or multiple specular components. The performance of a dual-hop decode-and-forward relaying system over multiple specular components fading channels(MSCC)with multiple Rayleigh distributed co-channel interferers in an interference-limited environment is investigated. The MSCC fading model is designed to allow direct and meaningful comparisons to be made between line-of-sight channels and non-line-of-sight channels, with exact parameter correspondences. Comparisons of outage and bit error performance between Nakagami-m/Rayleigh and MSCC/Rayleigh fading environments show that the MSCC model is needed to describe line-of-sight channels that cannot be accurately modeled by the Nakagami-m, or other fading models.展开更多
In this paper,we propose improved approaches for two-dimensional(2 D) direction-of-arrival(DOA) estimation for a uniform rectangular array(URA).Unlike the conventional eigenstructure-based estimation approaches such a...In this paper,we propose improved approaches for two-dimensional(2 D) direction-of-arrival(DOA) estimation for a uniform rectangular array(URA).Unlike the conventional eigenstructure-based estimation approaches such as Multiple Signals Classification(MUSIC) and Estimation of Signal Parameters via Rotational Invariance Technique(ESPRIT),the proposed approaches estimate signal and noise subspaces with Nystr?m approximation,which only need to calculate two sub-matrices of the whole sample covariance matrix and avoid the need to directly calculate the eigenvalue decomposition(EVD) of the sample covariance matrix.Hence,the proposed approaches can improve the computational efficiency greatly for large-scale URAs.Numerical results verify the reliability and efficiency of the proposed approaches.展开更多
Lactococcus lactis and Streptococcus thermophilus are considered as ideal chassis of engineered probiotics,while food-grade genetic tools are limited in those strains.Here,a Zn^(2+)-controlled gene expression(ZICE)sys...Lactococcus lactis and Streptococcus thermophilus are considered as ideal chassis of engineered probiotics,while food-grade genetic tools are limited in those strains.Here,a Zn^(2+)-controlled gene expression(ZICE)system was identified in the genome of S.thermophilus CGMCC7.179,including a transcriptional regulator sczAst and a promoter region of cation transporter czcD(PczcDst).Specific binding of the SczAst to the palindromic sequences in PczcDst was demonstrated by EMSA analysis,suggesting the regulation role of SczAst on PczcDst.To evaluate their possibility to control gene expression in vivo,the sczAst-PczcDst was employed to drive the expression of green fluorescence protein(GFP)gene in L.lactis NZ9000 and S.thermophilus CGMCC7.179,respectively.Both of the transformants could express GFP under Zn^(2+)induction,while no fluorescence without Zn^(2+)addition.For optimal conditions,Zn^(2+)was used at a final concentration of 0.8 mM in L.lactis and 0.16 mM in S.thermophilus at OD600 close to 0.4,and omitting yeast extract powder in the medium unexpectedly improved GFP expression level by 2.2-fold.With the help of the ZICE system,engineered L.lactis and S.thermophilus strains were constructed to secret cytokine interleukin-10(IL-10)with immunogenicity,and the IL-10 content in the supernatant of the engineered L.lactis was 59.37%of that under the nisin controlled expression system.This study provided a tightly controlled expression system by the food-grade inducer Zn^(2+),having potential in development of engineered probiotics.展开更多
Time difference of arrival(TDOA)is the positioning technique with the most potential in cellular mobile telecommunication systems.The Taylor series expansion method has been widely used in solving nonlinear equations ...Time difference of arrival(TDOA)is the positioning technique with the most potential in cellular mobile telecommunication systems.The Taylor series expansion method has been widely used in solving nonlinear equations for its high accuracy and good robustness.However,the performance of the Taylor’s method depends highly on the initial estimation.Therefore,one new algorithm,hybrid optimizing algo-rithm(HOA)was proposed,which combines the Taylor series expansion method with the steepest decent method.The steepest decent method features fast convergence at the initial iteration and small computation complexity.HOA takes great advantage of both methods.Simulation results show that HOA achieves better performance on positioning accuracy and efficiency.展开更多
基金supported by“the Fundamental Research Funds for the Central Universities No. 2017JBM016”
文摘Indoor localization has gained much attention over several decades due to enormous applications. However, the accuracy of indoor localization is hard to improve because the signal propagation has small scale effects which leads to inaccurate measurements. In this paper, we propose an efficient learning approach that combines grid search based kernel support vector machine and principle component analysis. The proposed approach applies principle component analysis to reduce high dimensional measurements. Then we design a grid search algorithm to optimize the parameters of kernel support vector machine in order to improve the localization accuracy. Experimental results indicate that the proposed approach reduces the localization error and improves the computational efficiency comparing with K-nearest neighbor, Back Propagation Neural Network and Support Vector Machine based methods.
基金supported by Fundamental Research Funds for the Central Universities No. 2014JBZ001the NSFC project No.11171016the National Program No.2015AA01A709
文摘Radio propagation in dense and super dense wireless networks as well as indoor-to-outdoor picocell networks can have multiple line-of-sight or multiple specular components. The performance of a dual-hop decode-and-forward relaying system over multiple specular components fading channels(MSCC)with multiple Rayleigh distributed co-channel interferers in an interference-limited environment is investigated. The MSCC fading model is designed to allow direct and meaningful comparisons to be made between line-of-sight channels and non-line-of-sight channels, with exact parameter correspondences. Comparisons of outage and bit error performance between Nakagami-m/Rayleigh and MSCC/Rayleigh fading environments show that the MSCC model is needed to describe line-of-sight channels that cannot be accurately modeled by the Nakagami-m, or other fading models.
基金supported by"the Fundamental Research Funds for the Central Universities No.2017JBM016"
文摘In this paper,we propose improved approaches for two-dimensional(2 D) direction-of-arrival(DOA) estimation for a uniform rectangular array(URA).Unlike the conventional eigenstructure-based estimation approaches such as Multiple Signals Classification(MUSIC) and Estimation of Signal Parameters via Rotational Invariance Technique(ESPRIT),the proposed approaches estimate signal and noise subspaces with Nystr?m approximation,which only need to calculate two sub-matrices of the whole sample covariance matrix and avoid the need to directly calculate the eigenvalue decomposition(EVD) of the sample covariance matrix.Hence,the proposed approaches can improve the computational efficiency greatly for large-scale URAs.Numerical results verify the reliability and efficiency of the proposed approaches.
基金financially supported by the National Natural Science Foundation of China(NSFC,Grant No.32372290)the National Key R&D Program of China(2019YFA0906700).
文摘Lactococcus lactis and Streptococcus thermophilus are considered as ideal chassis of engineered probiotics,while food-grade genetic tools are limited in those strains.Here,a Zn^(2+)-controlled gene expression(ZICE)system was identified in the genome of S.thermophilus CGMCC7.179,including a transcriptional regulator sczAst and a promoter region of cation transporter czcD(PczcDst).Specific binding of the SczAst to the palindromic sequences in PczcDst was demonstrated by EMSA analysis,suggesting the regulation role of SczAst on PczcDst.To evaluate their possibility to control gene expression in vivo,the sczAst-PczcDst was employed to drive the expression of green fluorescence protein(GFP)gene in L.lactis NZ9000 and S.thermophilus CGMCC7.179,respectively.Both of the transformants could express GFP under Zn^(2+)induction,while no fluorescence without Zn^(2+)addition.For optimal conditions,Zn^(2+)was used at a final concentration of 0.8 mM in L.lactis and 0.16 mM in S.thermophilus at OD600 close to 0.4,and omitting yeast extract powder in the medium unexpectedly improved GFP expression level by 2.2-fold.With the help of the ZICE system,engineered L.lactis and S.thermophilus strains were constructed to secret cytokine interleukin-10(IL-10)with immunogenicity,and the IL-10 content in the supernatant of the engineered L.lactis was 59.37%of that under the nisin controlled expression system.This study provided a tightly controlled expression system by the food-grade inducer Zn^(2+),having potential in development of engineered probiotics.
基金This work was supported by the Research on High-Speed Railway Intelligent Transportation Information System and Key Techniques(No.60332020).
文摘Time difference of arrival(TDOA)is the positioning technique with the most potential in cellular mobile telecommunication systems.The Taylor series expansion method has been widely used in solving nonlinear equations for its high accuracy and good robustness.However,the performance of the Taylor’s method depends highly on the initial estimation.Therefore,one new algorithm,hybrid optimizing algo-rithm(HOA)was proposed,which combines the Taylor series expansion method with the steepest decent method.The steepest decent method features fast convergence at the initial iteration and small computation complexity.HOA takes great advantage of both methods.Simulation results show that HOA achieves better performance on positioning accuracy and efficiency.