期刊文献+
共找到1篇文章
< 1 >
每页显示 20 50 100
Biosynthesis of antibiotic chuangxinmycin from Actinoplanes tsinanensis 被引量:2
1
作者 Yuanyuan Shi Zhibo Jiang +6 位作者 Xingxing Li Lijie Zuo Xuan Lei Liyan Yu linzhuan wu Jiandong Jiang Bin Hong 《Acta Pharmaceutica Sinica B》 SCIE CAS CSCD 2018年第2期283-294,共12页
Chuangxinmycin is an antibiotic isolated from Actinoplanes tsinanensis CPCC 200056 in the1970 s with a novel indole-dihydrothiopyran heterocyclic skeleton. Chuangxinmycin showed in vitro antibacterial activity and in ... Chuangxinmycin is an antibiotic isolated from Actinoplanes tsinanensis CPCC 200056 in the1970 s with a novel indole-dihydrothiopyran heterocyclic skeleton. Chuangxinmycin showed in vitro antibacterial activity and in vivo efficacy in mouse infection models as well as preliminary clinical trials.But the biosynthetic pathway of chuangxinmycin has been obscure since its discovery. Herein, we report the identification of a stretch of DNA from the genome of A. tsinanensis CPCC 200056 that encodes genes for biosynthesis of chuangxinmycin by bioinformatics analysis. The designated cxn cluster was then confirmed to be responsible for chuangxinmycin biosynthesis by direct cloning and heterologous expressing in Streptomyces coelicolor M1146. The cytochrome P450 CxnD was verified to be involved in the dihydrothiopyran ring closure reaction by the identification of seco-chuangxinmycin in S. coelicolor M1146 harboring the cxn gene cluster with an inactivated cxn D. Based on these results, a plausible biosynthetic pathway for chuangxinmycin biosynthesis was proposed, by hijacking the primary sulfur transfer system for sulfur incorporation. The identification of the biosynthetic gene cluster of chuangxinmycin paves the way for elucidating the detail biochemical machinery for chuangxinmycin biosynthesis, and provides the basis for the generation of novel chuangxinmycin derivatives by means of combinatorial biosynthesis and synthetic biology. 展开更多
关键词 Chuangxinmycin Actinoplanes tsinanensis Biosynthesis gene cluster Heterologous expression Cytochrome P450 Seco-chuangxinmycin C–S bond formation Sulfur incorporation
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部