Expression analysis of the R2R3-MYB gene family in upland cotton and functional study of GhMYB3D5 in regulating Verticillium wilt resistance

Improving plant resistance to Verticillium wilt(VW),which causes massive losses in Gossypium hirsutum,is a global challenge.Crop plants need to efficiently allocate their limited energy resources to maintain a balance between growth and defense.However,few transcriptional regulators specifically respond to Verticillium dahliae and the underlying mechanism has not been identified in cotton.In this study,we found that the that expression of most R2R3-MYB members in cotton is significantly changed by V.dahliae infection relative to the other MYB types.One novel R2R3-MYB transcription factor(TF)that specifically responds to V.dahliae,GhMYB3D5,was identified.GhMYB3D5 was not expressed in 15 cotton tissues under normal conditions,but it was dramatically induced by V.dahliae stress.We functionally characterized its positive role and underlying mechanism in VW resistance.Upon V.dahliae infection,the up-regulated GhMYB3D5 bound to the GhADH1 promoter and activated GhADH1expression.In addition,GhMYB3D5 physically interacted with GhADH1 and further enhanced the transcriptional activation of GhADH1.Consequently,the transcriptional regulatory module GhMYB3D5-GhADH1 then promoted lignin accumulation by improving the transcriptional levels of genes related to lignin biosynthesis(GhPAL,GhC4H,Gh4CL,and GhPOD/GhLAC)in cotton,thereby enhancing cotton VW resistance.Our results demonstrated that the GhMYB3D5 promotes defense-induced lignin accumulation,which can be regarded as an effective way to orchestrate plant immunity and growth.

New Identity Based Proxy Re-Encryption Scheme from Lattices

Identity-Based Proxy Re-Encryption (IB-PRE) allows a semi-trusted proxy to convert the ciphertext encrypted under Alice’s identity into Bob’s ciphertext of the same message without leaking plaintext. Lattice-based cryptography enjoys potential resistance to quantum analysis and low computational complexity. A multi-hop and unidirectional IB-PRE from lattices is presented. We split the functions of decryption and ciphertext transformation separately, and design the double private keys mechanism, where two keys are generated for each user, one key is used to decrypt the ciphertext by Pre-Image Sampling technique, and the other is used to generate the re-encryption key by Bonsai Trees technique. The generation of the re-encryption key is non-interactive and collusion resistant. Moreover, its IND-sID-CPA security over the decisional Learning With Errors (LWE) assumption under the standard model is proved. Compared with some previous IBPRE schemes from Bilinear Pairings, the format of transformed ciphertext in our scheme remains unchanged, furthermore, it can also resist quantum analysis. Compared with some existing IB-PRE schemes from lattices with similar properties, the space of the message in our scheme is a vector of length l and the encryption process remains a lower encryption blowup factor. At last, a proof-of-concept implementation is provided.

Health Management of Patients with Type II Diabetes and its Influence on Chronic Complication

Objectives:To explore the application of health management in patients with type II diabetes and to analyze and compare the incidence of chronic complications.Methods:the relevant research work was carried out in our hospital.During September 2018 to September 2019,100 patients with type II diabetes were randomly divided into two groups:one group was given routine nursing intervention,the other group was given health management,and they were named control group and experimental group respectively.Each group had 50 patients,the influence of different nursing methods on the incidence of chronic complications in patients was explored.Results:In the one-year followup results,the corresponding complications of patients mainly include cardiovascular disease,kidney disease,cerebrovascular disease,fundus disease and peripheral neuropathy.The incidence of complications in the experimental group is 10.00%,while that in the control group is 32.00%.in comparison,the incidence in the experimental group is lower,and the data difference between the two group is small(P<0.05),which exists significance.Conclusion:The application of health management method in type II diabetes has significant effect,which can reduce the incidence of chronic complications,improve the quality of life of patients,and has positive significance for clinical development.

Observation on the Therapeutic Effects of Wenjing Decoction on Menstrual Disorders

Objective:To treat patients with menstrual disorders with Wenjing Decoction and analyze its therapeutic effects.Metlods:80 patients with menstrual disorders were selected as the research subjects,and they were divided into two groups(experimental group and control group),with each group given different treatment methods.The control group was given conventional Western medical treatment while the experimental group was given Wenjing Decoction treatment,and the effects of different treatment methods on the patients were compared.Results:The total number of effectively treated patients in the experimental group(39 cases,97.50%)was more than the that of the control group(25 cases,62.50%)。and the quality of life score of the experimental group(91.56±4.44 points)was higher that of the control group(81.72±3.19 points),and the number of menstnual recovery cycles(1.11±0.41)was less than that of the control group patients(2.42±1.25 times),P<0.05.Conclusion:The application of Wenjing Decoction for patients with menstrual disorders can significantly improve the treatment efficacy and is beneficial to improve the quality of life of the patients and shorten the number of cycles that menstruation starts to return to normal.

Identity-based threshold proxy re-encryption scheme from lattices and its applications

Threshold proxy re-encryption(TPRE)can prevent collusion between a single proxy and a delegatee from converting arbitrary files against the wishes of the delegator through multiple proxies,and can also provide normal services even when certain proxy servers are paralyzed or damaged.A non-interactive identity-based TPRE(IB-TPRE)scheme over lattices is proposed which removes the public key certificates.To accomplish this scheme,Shamir’s secret sharing is employed twice,which not only effectively hides the delegator’s private key information,but also decentralizes the proxy power by splitting the re-encryption key.Robustness means that a combiner can detect a misbehaving proxy server that has sent an invalid transformed ciphertext share.This property is achieved by lattice-based fully homomorphic signatures.As a result,the whole scheme is thoroughly capable of resisting quantum attacks even when they are available.The security of the proposed scheme is based on the decisional learning with error hardness assumption in the standard model.Two typical application scenarios,including a file-sharing system based on a blockchain network and a robust key escrow system with threshold cryptography,are presented.

高血压肾损伤患者外周血单个核细胞PPARγ和NF-κB的mRNA表达及其与心血管重构的相关性

目的探讨高血压肾损伤患者外周血单个核细胞过氧化物酶体增殖物激活受体γ(PPARγ)和核因子-κB(NF-κB)的mRNA表达及其与心血管重构的相关性。方法选择2016年5月至2019年5月本院收治的高血压患者,65例为单纯高血压组,50例高血压肾损伤患者为高血压肾损伤组,健康者50例为对照组。测定各组受试者的外周血单个核细胞PPARγmRNA、NF-κB mRNA表达;应用心脏和血管超声分别检测颈动脉及心脏的重构指标:颈动脉平均内膜中层厚度(IMT)、颈动脉斑块积分、左心室质量指数(LVMI)。应用Pearson方法分析高血压肾损伤患者外周血单个核细胞PPARγmRNA、NF-κB mRNA表达水平与心血管重构指标的相关性,多元线性回归分析高血压肾损伤患者IMT的影响因素。结果与对照组相比,单纯高血压组、高血压肾损伤组患者的IMT、斑块积分、LVMI和NF-κB mRNA表达水平明显升高,而PPARγmRNA表达水平均明显降低(P<0.05)。高血压肾损伤组IMT、斑块积分、LVMI各指标和NF-κB mRNA表达水平均明显高于单纯高血压组,PPARγmRNA表达水平明显低于单纯高血压组(P<0.05)。高血压肾损伤患者PPARγmRNA表达水平均与IMT、斑块积分、LVMI呈负相关关系(均P<0.05);NF-κB mRNA表达水平均与IMT、斑块积分、LVMI均呈正相关关系(P均<0.05)。PPARγmRNA降低、NF-κB mRNA升高是高血压肾损伤患者IMT的影响因素(均P<0.05)。结论高血压肾损伤患者外周血PPARγmRNA表达明显降低,NF-κB mRNA表达明显升高,二者均与心血管重构有相关性。