Overexpression of amplified in breast cancer 1 (AIB1) gene promotes lung adenocarcinoma aggressiveness in vitro and in vivo by upregulating C-X-C motif chemokine receptor 4

Background:We previously found that overexpression of the gene known as amplified in breast cancer 1(AIB1)was associated with lymph node metastasis and poor prognosis in patients with lung adenocarcinoma.However,the role of AIB1 in that malignancy remains unknown.The present study aimed to investigate the function of AIB1 in the process of lung adenocarcinoma cell metastasis.Methods:A series of in vivo and in vitro assays were performed to elucidate the function of AIB1,while real-time PCR and Western blotting were utilized to identify the potential downstream targets of AIB1 in the process of lung adenocarcinoma metastasis.Rescue experiments and in vitro assays were performed to investigate whether the invasive-ness of AIB1-induced lung adenocarcinoma was mediated by C-X-C motif chemokine receptor 4(CXCR4).Results:The ectopic overexpression of AIB1 in lung adenocarcinoma cells substantially enhanced cell migration and invasive abilities in vitro and tumor metastasis in vivo,whereas the depletion of AIB1 expression substantially inhibited lung adenocarcinoma cell migration and invasion.CXCR4 was identified as a potential downstream target of AIB1 in lung adenocarcinoma.The knockdown of AIB1 greatly reduced CXCR4 gene expression at both the transcription and protein levels,whereas the knockdown of CXCR4 in cells with AIB1 ectopic overexpression diminished AIB1-induced migration and invasion in vitro and tumor metastasis in vivo.Furthermore,we found a significant positive association between the expression of AIB1 and CXCR4 in lung adenocarcinoma patients(183 cases),and the co-overexpression of AIB1 and CXCR4 predicted the poorest prognosis.Conclusions:These findings suggest that AIB1 promotes the aggressiveness of lung adenocarcinoma in vitro and in vivo by upregulating CXCR4 and that it might be usable as a novel prognostic marker and/or therapeutic target for this disease.

肠道膀胱准备下不同固定方式对前列腺癌精准放疗影响

目的比较3种体位固定装置在前列腺癌精准放疗中的摆位误差,为盆腔肿瘤精准放疗固定装置的选择和靶区外扩边界(MPTV)提供依据。方法回顾性分析中山大学肿瘤防治中心2015年4月至2020年12月133例需盆腔引流区照射的前列腺癌患者,采用1.2 m真空袋(39例)、1.8 m真空袋(44例)和本中心改进的个体化俯卧板(50例)固定。每位患者定位、放疗前均按流程进行肠道膀胱准备,每次治疗前锥形束CT与计划CT的配准采取相同配准框和算法,记录合格肠道膀胱的头脚、左右、腹背三个方向摆位误差,分析3种固定装置下3个方向摆位误差及相应MPTV数值,分析摆位误差与年龄、体重指数的相关性。结果3333组摆位误差数据得出,头脚、左右方向的1.2 m真空袋摆位误差均值(3.26、2.34 mm)均大于1.8 m真空袋(2.51、1.90 mm,P值均<0.001)和个体化俯卧板(3.07 mm,P=0.066;2.10 mm,P=0.009)。腹背方向的1.2 m真空袋(仰卧)摆位误差均值(2.20 mm)小于1.8 m真空袋(3.33 mm,P<0.001)和个体化俯卧板(3.61 mm,P<0.001)。1.8 m真空袋各方向摆位误差均小于个体化俯卧板(P≤0.028)。根据Van Herk外扩公式得出:1.2 m真空袋3个方向MPTV为4 mm左右,1.8 m真空袋和个体化俯卧板头脚、左右方向MPTV为3 mm左右,腹背方向>5 mm。摆位误差与年龄、BMI均不相关。结论摆位精准方面,1.8 m真空袋最优,个体化俯卧板次之;腹背方向仰卧位优于俯卧位。