Clinical Experience Sharing Of 218 Cases without Hemorrhage after Procedure for Prolapse and Hemorrhoids

Objective:To summarize the experience without hemorrhage after PPH of 218 patients with mixed hemorrhoid.Methods:PPH(Procedure for prolapse and hemorrhoids)was performed routinely.During operation,it is paid attention that the hemorrhoid artery should be completely mutilated,the anastomat should be squeezed before and after the anastomosis,the anastomosis should be carefully examined,the hemostasis should be completely performed,and relevant hemostasis measures should be taken after the operation.Results:None of the 218 patients had postoperative hemorrhage.Conclusion:Relevant measures can be taken after operation to prevent the occurrence of postoperative hemorrhage.

DISCRIMINATIVE ANALYSIS OF FUNCTIONAL NEAR-INFRARED SPECTROSCOPY SIGNALS FOR DEVELOPMENT OF NEUROIMAGING BIOMARKERS OF ELDERLY DEPRESSION

Functional near-infrared spectroscopy(fNIRS)is a neuroimaging technology which is suitable for psychiatric patients.Several fNIRS studies have found abnormal brain activations during cognitive tasks in elderly depression.In this paper,we proposed a discriminative model of multivariate pattern classification based on fNIRS signals to distinguish elderly depressed patients from healthy controls.This model used the brain activation patterns during a verbal fluency task as features of classification.Then Pseudo-Fisher Linear Discriminant Analysis was performed on the feature space to generate discriminative model.Using leave-one-out(LOO)cross-validation,our results showed a correct classification rate of 88%.The discriminative model showed its ability to identify people with elderly depression and suggested that fNIRS may be an efficient clinical tool for diagnosis of depression.This study may provide the first step for the development of neuroimaging biomarkers based on fNIRS in psychiatric disorders.

Clinical Significance of Serum Bilirubin in the Diagnosis of Atypical Acute Appendicitis

Objective:To investigate the significance of serum bilirubin in the diagnosis of atypical acute appendicitis.Methods:perform a retrospective analysis of 120 patients with atypical acute appendicitis diagnosed in our hospital from July 2012 to July 2016.All patients underwent routine liver function tests before surgery.Results:83 patients were confirmed by surgery as acute appendicitis,of which 68 patients had elevated serum total bilirubin and direct bilirubin,and 15 patients were normal;37 patients were confirmed by surgery as non-acute appendicitis,of which 7 patients had elevated serum total bilirubin and direct bilirubin,and 30 patients were normal.Conclusion:The increase of serum bilirubin level has certain clinical significance for the diagnosis of atypical acute appendicitis.

Extreme Heat Exposure Induced Acute Kidney Injury through NLRP3 Inflammasome Activation in Mice

Climate change has resulted in a marked increase in heat extremes that carry a severe risk for morbidity and mortality.Kidney is sensitive to heat stimulation,and acute kidney injury(AKI)is the early event.In this study,we investigated the adverse effects of heat extremes and their underlying mechanism.A total of 16 wild-type C57BL/6N mice were randomly divided into groups of control(exposed to 22±0.5°C)and heat(exposed to 39.5±0.5°C until the core body temperature reached the maximum).First,extreme heat exposure induced AKI evidenced by kidney dysfunction and morphological impairment.In addition,heat exposure suppressed expression of molecules for mitochondrial energetics and fatty acid beta-oxidation and disturbed the balance of oxidative stress in the kidney.Moreover,heat exposure enhanced the protein levels in the upstream signaling pathway for NLRP3 inflammasome formation,followed by NLRP3 inflammasome activation and inflammatory cytokine production.These findings demonstrated that acute extreme heat exposure may induce AKI through the NLRP3 inflammasome formation and activation.

Insight into the role of cholesterol in modulation of morphology and mechanical properties of CH0-K1 cells: An in situ AFM study

Cholesterol plays a significant role in the organization of lipids and modulation of membrane dynamics in mammalian cells. However, the effect of cholesterol depletion on the eukaryotic cell membranes seems controversial. In this study, the effects of cholesterol on the topography and mechanical behaviors of CHO-K1 cells with manipulated membrane cholesterol contents were investigated by atomic force microscopy (AFM) technique. Here, we found that the depletion of cholesterol in cell membranes could increase the membrane stiffness, reduce the cell height as well as promote cell retraction and detachment from the surface, whereas the cholesterol restoration could reverse the effect of cholesterol depletion on the membrane stiffness. Increased methyl-β-cyclodextrin levels and incubation time could significantly increase Young’s modulus and degree of stiffing on cell membrane and cytoskeleton. This research demonstratede importance of cholesterol in regulating the dynamics of cytoskeleton-mediated processes. AFM technique offers excellent advantages in the dynamic monitoring of the change in membranes mechanical properties and behaviors during the imaging process. This promising technology can be utilized in studying the membrane properties and elucidating the underlying mechanism of distinct cells in the near-native environment.