Prokaryotic gene expression is largely regulated on transcriptional levels with the involvement of promoters,RNA polymerase and sigma factors.Developing new promoters to customize gene transcriptional regulation becom...Prokaryotic gene expression is largely regulated on transcriptional levels with the involvement of promoters,RNA polymerase and sigma factors.Developing new promoters to customize gene transcriptional regulation becomes increasingly demanded in synthetic biology and biotechnology.In this study,we designed synthetic promoters in the Gram-positive model bacterium Bacillus subtilis by interlocking the binding motifs ofσA for house-keeping gene expression and that of two alternative sigma factorsσH andσB which are involved in responding post-exponential growth and general stress,respectively.The developed promoters are recognized by multiple sigma factors and hence generate strong transcriptional strength when host cells grow under normal or stressed conditions.With green fluorescent protein as the reporter,a set of strong promoters were identified,in which the transcription activities of PHA-1,PHAB-4,PHAB-7 were 18.6,4.1,3.3 fold of that of the commonly used promoter P43,respectively.Moreover,some of the promoters such as PHA-1,PHAB-4,PHAB-7,PBA-2 displayed increased transcriptional activities in response to high salinity or low pH.The promoters developed in this study should enrich the biotechnological toolboxes of B.subtilis.展开更多
基金This work was financially supported by the National Natural Science Foundation of China(31970085 and 31670092)the National Key R&D program of China(2018YFA0901401)+2 种基金the Fundamental Research Funds for the Central Universities(JUSRP51707A)a grant from the Key Technologies R&D Program of Jiangsu Province(BE2019630)the 111 Project.Y.W.was sponsored by the China Postdoctoral Science Foundation(2019M651702).
文摘Prokaryotic gene expression is largely regulated on transcriptional levels with the involvement of promoters,RNA polymerase and sigma factors.Developing new promoters to customize gene transcriptional regulation becomes increasingly demanded in synthetic biology and biotechnology.In this study,we designed synthetic promoters in the Gram-positive model bacterium Bacillus subtilis by interlocking the binding motifs ofσA for house-keeping gene expression and that of two alternative sigma factorsσH andσB which are involved in responding post-exponential growth and general stress,respectively.The developed promoters are recognized by multiple sigma factors and hence generate strong transcriptional strength when host cells grow under normal or stressed conditions.With green fluorescent protein as the reporter,a set of strong promoters were identified,in which the transcription activities of PHA-1,PHAB-4,PHAB-7 were 18.6,4.1,3.3 fold of that of the commonly used promoter P43,respectively.Moreover,some of the promoters such as PHA-1,PHAB-4,PHAB-7,PBA-2 displayed increased transcriptional activities in response to high salinity or low pH.The promoters developed in this study should enrich the biotechnological toolboxes of B.subtilis.
