Objective:To analyze the clinical features of asymptomatic patients infected with the SARS-CoV-2 novel coronavirus.Methods:The clinical data of 10 asymptomatic cases and 12 symptomatic cases of COVID-19 diagnosed duri...Objective:To analyze the clinical features of asymptomatic patients infected with the SARS-CoV-2 novel coronavirus.Methods:The clinical data of 10 asymptomatic cases and 12 symptomatic cases of COVID-19 diagnosed during February 2020 to April 2020 was collected and the clinical features of the two groups of patients were compared.Results:10 cases of asymptomatic infection and 12 cases of symptomatic patients were tested positive for the novel coronavirus nucleic acid test.There was no significant difference in gender distribution between the two groups(P>0.05);the average age of patients in the asymptomatic group was lower than that of the symptomatic group(P<0.05),the difference in clinical classification between the two groups was statistically significant(P<0.05);there was no statistically significant difference in the results of chest CT examination involving lung lobes between the two groups(P>0.05),and there was no statistically significant difference in mortality between the two groups(P>0.05).Conclusion:The average age of asymptomatic novel coronavirus infections was lower than that of confirmed cases of COVID-19,mainly among young people.There was no significant difference in clinical classification,mortality and chest CT examination results between symptomatic and asymptomatic cases.展开更多
Our recent studies demonstrate that the focal adhesion protein Kindlin-2 is critical for chondrogenesis and early skeletal development. Here, we show that deleting Kindlin-2 from osteoblasts using the 2.3-kb mouse Col...Our recent studies demonstrate that the focal adhesion protein Kindlin-2 is critical for chondrogenesis and early skeletal development. Here, we show that deleting Kindlin-2 from osteoblasts using the 2.3-kb mouse Col1 a1-Cre transgene minimally impacts bone mass in mice, but deleting Kindlin-2 using the 10-kb mouse Dmp1-Cre transgene, which targets osteocytes and mature osteoblasts, results in striking osteopenia in mice. Kindlin-2 loss reduces the osteoblastic population but increases the osteoclastic and adipocytic populations in the bone microenvironment. Kindlin-2 loss upregulates sclerostin in osteocytes,downregulates β-catenin in osteoblasts, and inhibits osteoblast formation and differentiation in vitro and in vivo. Upregulation ofβ-catenin in the mutant cells reverses the osteopenia induced by Kindlin-2 deficiency. Kindlin-2 loss additionally increases the expression of RANKL in osteocytes and increases osteoclast formation and bone resorption. Kindlin-2 deletion in osteocytes promotes osteoclast formation in osteocyte/bone marrow monocyte cocultures, which is significantly blocked by an anti-RANKLneutralizing antibody. Finally, Kindlin-2 loss increases osteocyte apoptosis and impairs osteocyte spreading and dendrite formation.Thus, we demonstrate an important role of Kindlin-2 in the regulation of bone homeostasis and provide a potential target for the treatment of metabolic bone diseases.展开更多
基金Autonomous Region's Key Research and Development Program of Science and Technology to Support the"Prevention and Control of the Pneumonia Pandemic due to Novel Coronavirus Infection"Special Project“Novel Coronavirus Infection Pneumonia”(2020BEG03057)Research on the Clinical Characteristics and Prevention and Treatment of Patients with Atypical Symptoms(2019-nCoV)Autonomous Region's Key Research and Development Program to Support the Special Project of"Prevention and Control of Pneumonia Pandemic due to Novel Coronavirus Infection","Research on Process Optimization of Pneumonia Screening,Quarantine and Quarantine Release of Novel Coronavirus Infection"(2020BEG03058)。
文摘Objective:To analyze the clinical features of asymptomatic patients infected with the SARS-CoV-2 novel coronavirus.Methods:The clinical data of 10 asymptomatic cases and 12 symptomatic cases of COVID-19 diagnosed during February 2020 to April 2020 was collected and the clinical features of the two groups of patients were compared.Results:10 cases of asymptomatic infection and 12 cases of symptomatic patients were tested positive for the novel coronavirus nucleic acid test.There was no significant difference in gender distribution between the two groups(P>0.05);the average age of patients in the asymptomatic group was lower than that of the symptomatic group(P<0.05),the difference in clinical classification between the two groups was statistically significant(P<0.05);there was no statistically significant difference in the results of chest CT examination involving lung lobes between the two groups(P>0.05),and there was no statistically significant difference in mortality between the two groups(P>0.05).Conclusion:The average age of asymptomatic novel coronavirus infections was lower than that of confirmed cases of COVID-19,mainly among young people.There was no significant difference in clinical classification,mortality and chest CT examination results between symptomatic and asymptomatic cases.
基金supported, in part, by the National Natural Science Foundation of China Grants (81991513, 81630066 and 81870532)Guangdong Provincial Science and Technology Innovation Council Grant (2017B030301018)Shenzhen Municipal Science and Technology Innovation Council Grants (KQJSCX20180319114434843, JCYJ20180302174117738, JCYJ20180302174246105, JSGG20180503182321166, JCYJ20150331101823686, and JCYJ20150831142427959)
文摘Our recent studies demonstrate that the focal adhesion protein Kindlin-2 is critical for chondrogenesis and early skeletal development. Here, we show that deleting Kindlin-2 from osteoblasts using the 2.3-kb mouse Col1 a1-Cre transgene minimally impacts bone mass in mice, but deleting Kindlin-2 using the 10-kb mouse Dmp1-Cre transgene, which targets osteocytes and mature osteoblasts, results in striking osteopenia in mice. Kindlin-2 loss reduces the osteoblastic population but increases the osteoclastic and adipocytic populations in the bone microenvironment. Kindlin-2 loss upregulates sclerostin in osteocytes,downregulates β-catenin in osteoblasts, and inhibits osteoblast formation and differentiation in vitro and in vivo. Upregulation ofβ-catenin in the mutant cells reverses the osteopenia induced by Kindlin-2 deficiency. Kindlin-2 loss additionally increases the expression of RANKL in osteocytes and increases osteoclast formation and bone resorption. Kindlin-2 deletion in osteocytes promotes osteoclast formation in osteocyte/bone marrow monocyte cocultures, which is significantly blocked by an anti-RANKLneutralizing antibody. Finally, Kindlin-2 loss increases osteocyte apoptosis and impairs osteocyte spreading and dendrite formation.Thus, we demonstrate an important role of Kindlin-2 in the regulation of bone homeostasis and provide a potential target for the treatment of metabolic bone diseases.