Effects of salvianolic acid-A on NIH/3T3 fibroblast proliferation,collagen synthesis and gene expression

AIM To investigate the mechanisms ofsalvianolic acid A（SA-A）against liver fibrosisin vitro.METHODS NIH/3T3 fibroblasts were culturedroutinely,and incubated with 10-4mol/L-10-7mol/L SA-A for 22 h.The cell viability wasassayed by[3H]proline incorporation,cellproliferation by[3H]TdR incorporation,cellcollagen synthetic rate was measured with[3H]proline impulse and collagenase digestionmethod.The total RNA was prepared from thecontrol cells and the drug treated cellsrespectively,and α（1）I pro-collagen mRNAexpression was semi-quantitatively analyzedwith RT-PCR.RESULTS 10-4mol/L SA-A decreased cellviability and exerted some cytotoxiciy,while10-5mol/L-10-7mol/L SA-A did not affect cellviability,but inhibited cell proliferationsignificantly,and 10-6mol/L SA-A had the besteffect on cell viability among theseconcentrations of drugs.10-5mol/L-10-6mol/LSA-A inhibited intracellular collagen syntheticrate,but no significant influence on extracellularcollagen secretion.Both 10-5mol/L and10-6mol/L SA-A could decrease α（1）I pro-collagen mRNA expression remarkably.CONCLUSION SA-A had potent action againstliver fibrosis.It inhibited NIH/3T3 fibroblastproliferation,intracellular collagen syntheticrate and type I pro-collagen gene expression,which may be one of the main mechanisms of thedrug.

Protective actions of salvianolic acid A on hepatocyte injured by peroxidation in vitro

INTRODUCTION Salvianolic radix,one of the most commonly usedtraditional Chinese herbs,was widely studied aboutits actions against liver injury and fibrosis,and wasone of the focuses of recent research.Salvianolic acid-A(SA-A)was an aqueous solublecomponent of Salvianolic radix.In our

简单无创评分系统和组织学评分预测非酒精性脂肪性肝病患者死亡风险:一项系统综述和Meta分析

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急性缺血性脑卒中后低脉压与不良预后相关

众所周知,脉压与心脏功能、动脉僵硬度、体液状态和血管事件有关。该研究旨在探讨基于中国台湾地区脑卒中登记资料的急性缺血性脑卒中（acute ischemic stroke,AIS）患者入院时脉压的预后作用。

腹壁子宫内膜异位症病灶透明细胞改变1例

腹壁子宫内膜异位症是指具有生长功能的子宫内膜异位到腹壁组织中,多见于剖宫产术后,常表现为术后逐渐增大的触痛结节。确诊后仍以手术治疗为主。对于短时间内复发或生长迅速的腹壁结节,应警惕细胞化生及恶变可能。因其缺乏特异性,病理诊断为金标准,故诊断往往具有滞后性。本文对我院收治的腹壁子宫内膜异位症病灶透明细胞改变1例进行分析并结合文献讨论子宫内膜异位症透明细胞改变的病理特征,腹壁内膜异位症相关病变的临床诊断及治疗,以提高对这类疾病的认识。