为鉴定EMS突变的真实性,本研究利用SSR标记和90 K SNP芯片对小麦品系H261及其EMS突变体进行检测。SSR检测结果表明,H261与LF2010和LF2099的差异SSR标记为0个,但与LF2100的差异SSR标记为10个,多态性比例为47.62%。SNP芯片分析结果表明,H...为鉴定EMS突变的真实性,本研究利用SSR标记和90 K SNP芯片对小麦品系H261及其EMS突变体进行检测。SSR检测结果表明,H261与LF2010和LF2099的差异SSR标记为0个,但与LF2100的差异SSR标记为10个,多态性比例为47.62%。SNP芯片分析结果表明,H261与LF2010和LF2099之间的差异位点分别为66和12个,分别占总数的0.080 9%和0.014 7%,2个突变体与H261的纯合差异SNP数目均为0;而H261与LF2100之间的差异位点为2 846个,占总数的3.487 9%,二者之间纯合差异SNP为784,占总数的0.960 8%。综上所述,LF2010和LF2099突变体与亲本H261的遗传背景高度一致,是H261经过EMS诱变的后代,而LF2100是天然异交或机械混杂产生的假突变体。本研究结果为更好地发挥小麦突变体在遗传改良和功能基因组研究奠定了一定的理论基础。展开更多
Aerospace provides a special environment with strong cosmic radiation,microgravity,weak geomagnetic field,supervacuum and superclean,which might affect plant growth and development as well as induce genetic changeds o...Aerospace provides a special environment with strong cosmic radiation,microgravity,weak geomagnetic field,supervacuum and superclean,which might affect plant growth and development as well as induce genetic changeds of crop seeds.Since 987,eitht recoverable satellites and five high altitude balloons have been used by the Chinese scientists to carry plant seeds.展开更多
文摘Aerospace provides a special environment with strong cosmic radiation,microgravity,weak geomagnetic field,supervacuum and superclean,which might affect plant growth and development as well as induce genetic changeds of crop seeds.Since 987,eitht recoverable satellites and five high altitude balloons have been used by the Chinese scientists to carry plant seeds.
