Caspase-8 is an initiator of apoptotic signalling,and aberrations in procaspase-8 have been verified to be associated with malignant tumours.However,it remains to be confirmed whether chemotherapy resistance is relate...Caspase-8 is an initiator of apoptotic signalling,and aberrations in procaspase-8 have been verified to be associated with malignant tumours.However,it remains to be confirmed whether chemotherapy resistance is related to procaspase-8 aberrations in acute myeloid leukaemia(AML)patients.Procaspase-8 WT or mutant plasmids were constructed with molecular techniques and were transfected into HeLa cells with Lipofectamine.Apoptosis analysis was performed with annexin V-FITC in the cells overexpressing the WT or mutant procaspase-8 with or without TRAIL treatment.The cleavage of procaspase-8 or the expression of proteins downstream in the apoptotic cascade was detected by Western blotting.The dimerization of WT and mutant procaspase-8 proteins w as demonstrated with Co-IP.展开更多
文摘Caspase-8 is an initiator of apoptotic signalling,and aberrations in procaspase-8 have been verified to be associated with malignant tumours.However,it remains to be confirmed whether chemotherapy resistance is related to procaspase-8 aberrations in acute myeloid leukaemia(AML)patients.Procaspase-8 WT or mutant plasmids were constructed with molecular techniques and were transfected into HeLa cells with Lipofectamine.Apoptosis analysis was performed with annexin V-FITC in the cells overexpressing the WT or mutant procaspase-8 with or without TRAIL treatment.The cleavage of procaspase-8 or the expression of proteins downstream in the apoptotic cascade was detected by Western blotting.The dimerization of WT and mutant procaspase-8 proteins w as demonstrated with Co-IP.
