Background:Verticillium wilt,caused by the soil-borne fungus of Verticillium dahliae Kleb.,is one of the most devastating diseases of cotton.The complex mechanism underlying cotton resistance to Verticillium wilt rema...Background:Verticillium wilt,caused by the soil-borne fungus of Verticillium dahliae Kleb.,is one of the most devastating diseases of cotton.The complex mechanism underlying cotton resistance to Verticillium wilt remains uncharacterized.Identifying an endogenous resistance gene may be helpful to control this disease.Previous studies revealed that succinate dehydrogenase(SDH)is involved in reactive oxygen species(ROS)-induced stress signaling pathway that is likely to be triggered by salicylic acid(SA).Here,through the metabolomics and differential expression analyses in wilt-inoculated cotton(Gossypium hirsutum),we noticed that Gh SDH1–1 gene in cotton may play an important role in the resistance to V.dahlia.Then we reported Gh SDH1–1 gene and its functional analysis in relation to the resistance of cotton to V.dahliae.Results:The Gh SDH1–1 gene in cotton root was significantly up-regulated after V.dahlia inoculation,and its expression level peaked at 12 and 24 h post-infection.SA can also induce the up-regulation of Gh SDH1–1.Additionally,the functional analysis showed that Gh SDH1–1-silenced cotton was more susceptible to V.dahliae than the control because of the significant decrease in abundance of immune-related molecules and severe damage to the SA-signaling pathway.In Arabidopsis thaliana,high expression of Gh SDH1–1 conferred high resistance to V.dahliae.Arabidopsis that overexpressed Gh SDH1–1 had higher resistance to V.dahliae infection compared with the wild-type.Conclusions:Our findings provide new insights into the role of Gh SDH1–1;it positively regulates cotton resistance to Verticillium wilt.The regulatory mechanism of Gh SDH1–1 is closely related to SA-related signaling pathway.展开更多
高压直流(high-voltage direct current,HVDC)传输系统受端近区交流电网故障可引起直流连续换相失败,甚至导致直流闭锁或电压崩溃事故,引起大面积停电风险。为实现动态调节直流首次换相失败恢复过程中的直流电流指令,以达到抑制直流连...高压直流(high-voltage direct current,HVDC)传输系统受端近区交流电网故障可引起直流连续换相失败,甚至导致直流闭锁或电压崩溃事故,引起大面积停电风险。为实现动态调节直流首次换相失败恢复过程中的直流电流指令,以达到抑制直流连续换相失败的目的,提出基于电压限流参数优化的直流传输系统换相失败抑制策略。在分析引起直流连续换相失败影响因素及逆变侧换流站与外部电网无功交互特性的基础上,以低压限流环节作为研究对象,采用粒子群算法对低压限流控制环节进行多分段拐点参数优化,克服常规低压限流环节单一线性的缺陷,实现在受端交流系统发生故障时逆变侧换流站的动态无功需求调整,进而抑制直流连续换相失败。最后通过仿真对比证明所提方法的有效性。展开更多
基金supported by the National Natural Science Foundation of China(31701479)。
文摘Background:Verticillium wilt,caused by the soil-borne fungus of Verticillium dahliae Kleb.,is one of the most devastating diseases of cotton.The complex mechanism underlying cotton resistance to Verticillium wilt remains uncharacterized.Identifying an endogenous resistance gene may be helpful to control this disease.Previous studies revealed that succinate dehydrogenase(SDH)is involved in reactive oxygen species(ROS)-induced stress signaling pathway that is likely to be triggered by salicylic acid(SA).Here,through the metabolomics and differential expression analyses in wilt-inoculated cotton(Gossypium hirsutum),we noticed that Gh SDH1–1 gene in cotton may play an important role in the resistance to V.dahlia.Then we reported Gh SDH1–1 gene and its functional analysis in relation to the resistance of cotton to V.dahliae.Results:The Gh SDH1–1 gene in cotton root was significantly up-regulated after V.dahlia inoculation,and its expression level peaked at 12 and 24 h post-infection.SA can also induce the up-regulation of Gh SDH1–1.Additionally,the functional analysis showed that Gh SDH1–1-silenced cotton was more susceptible to V.dahliae than the control because of the significant decrease in abundance of immune-related molecules and severe damage to the SA-signaling pathway.In Arabidopsis thaliana,high expression of Gh SDH1–1 conferred high resistance to V.dahliae.Arabidopsis that overexpressed Gh SDH1–1 had higher resistance to V.dahliae infection compared with the wild-type.Conclusions:Our findings provide new insights into the role of Gh SDH1–1;it positively regulates cotton resistance to Verticillium wilt.The regulatory mechanism of Gh SDH1–1 is closely related to SA-related signaling pathway.
文摘高压直流(high-voltage direct current,HVDC)传输系统受端近区交流电网故障可引起直流连续换相失败,甚至导致直流闭锁或电压崩溃事故,引起大面积停电风险。为实现动态调节直流首次换相失败恢复过程中的直流电流指令,以达到抑制直流连续换相失败的目的,提出基于电压限流参数优化的直流传输系统换相失败抑制策略。在分析引起直流连续换相失败影响因素及逆变侧换流站与外部电网无功交互特性的基础上,以低压限流环节作为研究对象,采用粒子群算法对低压限流控制环节进行多分段拐点参数优化,克服常规低压限流环节单一线性的缺陷,实现在受端交流系统发生故障时逆变侧换流站的动态无功需求调整,进而抑制直流连续换相失败。最后通过仿真对比证明所提方法的有效性。
