Calcium(Ca^(2+))plays an important role in determining plant growth and development because it maintains cell wall and membrane integrity.Therefore,understanding the role of Ca^(2+)in carbon and lipid metabolism could...Calcium(Ca^(2+))plays an important role in determining plant growth and development because it maintains cell wall and membrane integrity.Therefore,understanding the role of Ca^(2+)in carbon and lipid metabolism could provide insights into the dynamic changes in cell membranes and cell walls during the rapid elongation of cotton fibers.In the present study,we found that the lack of Ca^(2+)promoted fiber elongation and rapid ovule expansion,but it also caused tissue browning in the ovule culture system.RNA-sequencing revealed that Ca^(2+)deficiency induced cells to be highly oxidized,and the expression of genes related to carbon metabolism and lipid metabolism was activated significantly.All gene members of nine key enzymes involved in glycolysis were up-regulated,and glucose was significantly reduced in Ca^(2+)deficiency-treated tissues.Ca^(2+)deficiency adjusted the flowing of glycolysis metabolic.However,low K^(+)recovered the expression levels of glycolysis genes and glucose content caused by Ca^(2+)deficiency.Electrospray ionizationtandem mass spectrometry technology was applied to uncover the dynamic profile of lipidome under Ca^(2+)and K^(+)interacted conditions.Ca^(2+)deficiency led to the decrease of fatty acid(FA),diacylglycerol(DAG),glycolipid and the significant increase of triacylglycerol(TAG),phospholipid phosphatidylethanolamine(PE),phosphatidylglycerol(PG),and PC(phosphatidylcholine).Low K^(+)restored the contents of FA,phospholipids,and glycolipids,effectively relieved the symptoms caused by Ca^(2+)deficiency,and recovered the development of fiber cells.This study revealed dynamic changes in transcript and metabolic levels and uncovered the signaling interaction of Ca^(2+)deficiency and low K^(+)in glycolysis and lipid metabolism during fiber development.展开更多
目的:系统评价葛根素治疗糖尿病视网膜病变的有效性。方法:计算机检索PubMed、Web of Science、Cochrane Library、CNKI、万方和维普数据库,搜集关于葛根素治疗糖尿病视网膜病变的随机对照试验(RCT),检索时限均从建库至2022年7月18日。...目的:系统评价葛根素治疗糖尿病视网膜病变的有效性。方法:计算机检索PubMed、Web of Science、Cochrane Library、CNKI、万方和维普数据库,搜集关于葛根素治疗糖尿病视网膜病变的随机对照试验(RCT),检索时限均从建库至2022年7月18日。由2位评价员独立筛选文献、提取资料并评价纳入研究的偏倚风险后,采用RevMan 5.3软件进行Meta分析。结果:共纳入6个研究,包括365例患者。Meta分析结果显示,与对照组相比,葛根素组在提高患者综合疗效有效率方面差异有统计学意义[OR=3.15,95%CI(1.88,5.28),P<0.0001];两组在提高患者视力水平方面差异有统计学意义[MD=0.73,95%CI(0.44,1.01),P<0.00001],使用葛根素组视力改善效果有效率明显高于未使用葛根素组。在调节血液流变学指标方面,在控制全血粘度(高切)[MD=-0.19,95%CI(-3.17,2.78),P=0.90]水平方面差异无统计学意义。调节全血粘度[MD=-0.75,95%CI(-1.04,-0.46),P<0.00001]、全血粘度(低切)[MD=-0.75,95%CI(-1.04,-0.46),P<0.00001]、血浆比粘度[MD=0.13,95%CI(0.05,0.20),P=0.01]、血沉[MD=-1.60,95%CI(-2.97,-0.24),P=0.02]、红细胞聚集指数[MD=-1.20,95%CI(-1.33,-1.07),P<0.00001]水平上差异有统计学意义。结论:现有证据表明,葛根素单用或联合常规治疗糖尿病视网膜病变可提高综合疗效有效率和改善视力水平、血液流变学水平(除全血比粘度(高切)以外)。但受纳入研究数量和质量的限制,上述结论尚待更多高质量研究予以验证。展开更多
【目的】体质指数(BMI)是衡量绵羊体况胖瘦程度的重要指标,与繁殖力等重要经济性状具有显著的相关。研究中国绵羊体质指数现状及其影响因素,对于绵羊资源评估、选育和生产管理都有重要的参考价值。【方法】测定湖羊和乌珠穆沁羊的成年...【目的】体质指数(BMI)是衡量绵羊体况胖瘦程度的重要指标,与繁殖力等重要经济性状具有显著的相关。研究中国绵羊体质指数现状及其影响因素,对于绵羊资源评估、选育和生产管理都有重要的参考价值。【方法】测定湖羊和乌珠穆沁羊的成年体重和体长;通过文献挖掘42个中国地方绵羊品种成年体重和体长数据以及年平均气温(AAT)、无霜期(FFP)、年平均降水量(AAP)、相对湿度(RH)、年平均日照时数(AASD)5个环境因子。分别计算各个品种的体质指数,分析体质指数与性别、品种类型、纬度、海拔及环境因子间的关系。【结果】中国绵羊BMI平均为100.871±20.488(公母分别为107.249 VS 94.494)。其中,蒙系绵羊BMI为101.450±21.315(公母分别为107.847 VS 95.052);藏系绵羊BMI为93.330±7.383(公母分别为95.156 VS 91.503);哈萨克系绵羊BMI为107.361±25.621(公母分别为118.254 VS 96.468)。公羊BMI显著大于母羊(P<0.05)。纬度越高,BMI越大(P<0.05)。海拔对BMI的影响不显著(P>0.05)。BMI与环境因子的回归方程为BMI=2.151E-15-0.347AAP+0.585RH+0.302AASD(R2=0.194)。【结论】中国地方绵羊品种的BMI变异丰富,公羊极显著高于母羊。纬度越高,地方绵羊品种的BMI越大,而各种环境因素对BMI具有不同程度影响。展开更多
基金supported by the National Natural Science Foundation of China(31901577)the Fundamental Research Funds for the Central Universities,China(SWU-KT22035)the State Key Laboratory of Cotton Biology Open Fund,China(CB2021A32)。
文摘Calcium(Ca^(2+))plays an important role in determining plant growth and development because it maintains cell wall and membrane integrity.Therefore,understanding the role of Ca^(2+)in carbon and lipid metabolism could provide insights into the dynamic changes in cell membranes and cell walls during the rapid elongation of cotton fibers.In the present study,we found that the lack of Ca^(2+)promoted fiber elongation and rapid ovule expansion,but it also caused tissue browning in the ovule culture system.RNA-sequencing revealed that Ca^(2+)deficiency induced cells to be highly oxidized,and the expression of genes related to carbon metabolism and lipid metabolism was activated significantly.All gene members of nine key enzymes involved in glycolysis were up-regulated,and glucose was significantly reduced in Ca^(2+)deficiency-treated tissues.Ca^(2+)deficiency adjusted the flowing of glycolysis metabolic.However,low K^(+)recovered the expression levels of glycolysis genes and glucose content caused by Ca^(2+)deficiency.Electrospray ionizationtandem mass spectrometry technology was applied to uncover the dynamic profile of lipidome under Ca^(2+)and K^(+)interacted conditions.Ca^(2+)deficiency led to the decrease of fatty acid(FA),diacylglycerol(DAG),glycolipid and the significant increase of triacylglycerol(TAG),phospholipid phosphatidylethanolamine(PE),phosphatidylglycerol(PG),and PC(phosphatidylcholine).Low K^(+)restored the contents of FA,phospholipids,and glycolipids,effectively relieved the symptoms caused by Ca^(2+)deficiency,and recovered the development of fiber cells.This study revealed dynamic changes in transcript and metabolic levels and uncovered the signaling interaction of Ca^(2+)deficiency and low K^(+)in glycolysis and lipid metabolism during fiber development.
文摘目的:系统评价葛根素治疗糖尿病视网膜病变的有效性。方法:计算机检索PubMed、Web of Science、Cochrane Library、CNKI、万方和维普数据库,搜集关于葛根素治疗糖尿病视网膜病变的随机对照试验(RCT),检索时限均从建库至2022年7月18日。由2位评价员独立筛选文献、提取资料并评价纳入研究的偏倚风险后,采用RevMan 5.3软件进行Meta分析。结果:共纳入6个研究,包括365例患者。Meta分析结果显示,与对照组相比,葛根素组在提高患者综合疗效有效率方面差异有统计学意义[OR=3.15,95%CI(1.88,5.28),P<0.0001];两组在提高患者视力水平方面差异有统计学意义[MD=0.73,95%CI(0.44,1.01),P<0.00001],使用葛根素组视力改善效果有效率明显高于未使用葛根素组。在调节血液流变学指标方面,在控制全血粘度(高切)[MD=-0.19,95%CI(-3.17,2.78),P=0.90]水平方面差异无统计学意义。调节全血粘度[MD=-0.75,95%CI(-1.04,-0.46),P<0.00001]、全血粘度(低切)[MD=-0.75,95%CI(-1.04,-0.46),P<0.00001]、血浆比粘度[MD=0.13,95%CI(0.05,0.20),P=0.01]、血沉[MD=-1.60,95%CI(-2.97,-0.24),P=0.02]、红细胞聚集指数[MD=-1.20,95%CI(-1.33,-1.07),P<0.00001]水平上差异有统计学意义。结论:现有证据表明,葛根素单用或联合常规治疗糖尿病视网膜病变可提高综合疗效有效率和改善视力水平、血液流变学水平(除全血比粘度(高切)以外)。但受纳入研究数量和质量的限制,上述结论尚待更多高质量研究予以验证。
文摘【目的】体质指数(BMI)是衡量绵羊体况胖瘦程度的重要指标,与繁殖力等重要经济性状具有显著的相关。研究中国绵羊体质指数现状及其影响因素,对于绵羊资源评估、选育和生产管理都有重要的参考价值。【方法】测定湖羊和乌珠穆沁羊的成年体重和体长;通过文献挖掘42个中国地方绵羊品种成年体重和体长数据以及年平均气温(AAT)、无霜期(FFP)、年平均降水量(AAP)、相对湿度(RH)、年平均日照时数(AASD)5个环境因子。分别计算各个品种的体质指数,分析体质指数与性别、品种类型、纬度、海拔及环境因子间的关系。【结果】中国绵羊BMI平均为100.871±20.488(公母分别为107.249 VS 94.494)。其中,蒙系绵羊BMI为101.450±21.315(公母分别为107.847 VS 95.052);藏系绵羊BMI为93.330±7.383(公母分别为95.156 VS 91.503);哈萨克系绵羊BMI为107.361±25.621(公母分别为118.254 VS 96.468)。公羊BMI显著大于母羊(P<0.05)。纬度越高,BMI越大(P<0.05)。海拔对BMI的影响不显著(P>0.05)。BMI与环境因子的回归方程为BMI=2.151E-15-0.347AAP+0.585RH+0.302AASD(R2=0.194)。【结论】中国地方绵羊品种的BMI变异丰富,公羊极显著高于母羊。纬度越高,地方绵羊品种的BMI越大,而各种环境因素对BMI具有不同程度影响。
