Discuss a class of real planar cubic systems with a critical point O (0,0) of nine orders and obtain the conditions for its limit cycle surrounding the origin, and prove that when small pertubations of coefficients ar...Discuss a class of real planar cubic systems with a critical point O (0,0) of nine orders and obtain the conditions for its limit cycle surrounding the origin, and prove that when small pertubations of coefficients are made, the critical point O (0,0) of nine orders is split into nine real simple critical points and the limit cycle surrounding the origin becomes the limit cycle containing nine critical points in its interior.展开更多
Objective: To investigate the potential antifibrotic mechanisms of Chinese medicine Ganshuang Granules(肝爽颗粒, GSG) and to provide clinical therapeutic evidence of its effects. Methods: A cirrhotic mouse model was e...Objective: To investigate the potential antifibrotic mechanisms of Chinese medicine Ganshuang Granules(肝爽颗粒, GSG) and to provide clinical therapeutic evidence of its effects. Methods: A cirrhotic mouse model was established by intraperitoneally injecting a mixture of CCl_4(40%) and oil(60%) at 0.2 mL per 100 g of body weight twice a week for 12 weeks. After 12-week modeling, GSG was intragastric administrated to the mice for 2 weeks, and the mice were divided into low-, medium-and high-dose groups at doses of 1, 2 and 4 g/(kg·day), respectively. Liver morphology changes were observed using Masson's trichrome staining and B-ultrasound. The levels of alanine aminotransferase(ALT), aspartate aminotransferase(AST) and hyaluronic acid(HA) in serum were detected using an automatic biochemistry analyzer. The expressions of desmin, smooth muscle actin(SMA) and Foxp3 in liver were detected by immunofluorescence. The regulatory T cell(Treg) frequency was determined through flow cytometry analysis. Collagen-Ⅰ, SMA, IL-6, tumor necrosis factor α(TNF-α), interleukin(IL)-1β and transforming growth factor β1(TGF-β1) expression levels were measured using quantitative polymerase chain reaction(qPCR). Results: Masson's staining result showed fewer pseudolobule structures and fibrous connective tissue in the GSG-treatment groups than in the spontaneous recovery group. Ultrasonography showed that GSG treatment reduced the number of punctate hyperechoic lesions in mice cirrhotic livers. The serum ALT, AST, HA levels were significantly ameliorated by GSG treatment(ALT: F=8.104, P=0.000; AST: F=7.078, P=0.002; and HA: F=7.621, P=0.001). The expression levels of collagen-Ⅰ and SMA in the cirrhotic livers were also attenuated by GSG treatment(collagen-Ⅰ: F=3.938, P=0.011; SMA: F=4.115, P=0.009). Tregs, which were elevated in the fibrotic livers, were suppressed by GSG treatment(F=8.268, P=0.001). The expressions of IL-6, TNF-α and IL-1β increased, and TGF-β levels decreased in the cirrhotic livers after GSG treatment(IL-6: F=5.457, P=0.004; TNF-α: F=6.023, P=0.002; IL-1β: F=6.658, P=0.001; and TGF-β1: F=11.239, P=0.000). Conclusions: GSG promoted the resolution/regression of cirrhosis and restored liver functions in part by suppressing Treg cell differentiation, which may be mediated by hepatic stellate cells.展开更多
目的分析出生时维生素D水平与早产儿缺氧缺血性脑病(HIE)的相关性,为预防早产儿HIE提供依据。方法选择博罗县妇幼保健计划生育服务中心2018年6月—2020年6月诊断为HIE的早产儿25例(HIE组)和未发生HIE的早产儿138例(非HIE组),比较两组母...目的分析出生时维生素D水平与早产儿缺氧缺血性脑病(HIE)的相关性,为预防早产儿HIE提供依据。方法选择博罗县妇幼保健计划生育服务中心2018年6月—2020年6月诊断为HIE的早产儿25例(HIE组)和未发生HIE的早产儿138例(非HIE组),比较两组母亲和早产儿出生时血清25-(OH)D的水平,并对HIE的危险因素进行Logistic回归分析。结果与非HIE组比较,HIE组早产儿宫内窘迫发生率较高、1 min Apgar评分较低、机械通气时间较长,差异均有统计学意义(χ^(2)=46.875,t=10.854、7.166,P<0.01);HIE组母亲和早产儿出生时血清25-(OH)D水平明显低于非HIE组,差异有统计学意义(t=2.346、2.092,P<0.05),HIE组早产儿出生时维生素D缺乏或不足的比例(64%)明显高于非HIE组(39.9%),差异有统计学意义(χ^(2)=5.019,P<0.05);两组母亲血清25-(OH)D水平与早产儿出生时25-(OH)D水平呈正相关(HIE组r=0.825,非HIE组r=0.682,P<0.05)。Logistic回归分析结果显示HIE的危险因素包括宫内窘迫(OR=1.137,95%CI:1.105~1.209)、1 min低Apgar评分(OR=1.170,95%CI:1.085~1.443)、机械通气时间(OR=1.431,95%CI:1.275~1.610)和低25-(OH)D水平(母亲OR=1.234,95%CI:1.010~1.511;早产儿OR=1.505,95%CI:1.191~1.899)。结论早产儿出生时维生素D缺乏或不足的比例达50.9%(83/163),母亲及早产儿出生时低维生素D水平是早产儿HIE发生的高危因素,应注重孕期和早产儿出生后的维生素D补充。展开更多
基金The Natural Science Foundation of Hunan Province !(No .97JJN 70 )
文摘Discuss a class of real planar cubic systems with a critical point O (0,0) of nine orders and obtain the conditions for its limit cycle surrounding the origin, and prove that when small pertubations of coefficients are made, the critical point O (0,0) of nine orders is split into nine real simple critical points and the limit cycle surrounding the origin becomes the limit cycle containing nine critical points in its interior.
基金Supported by the China National Key Project of Twelfth FiveYear Plan(Nos.2012ZX10002004-006,2012ZX10004904-003-001,and 2013ZX10002002-006-001)National Natural Science Foundation of China(No.81300349)+2 种基金Beijing Natural Science Foundation(No.7144216)Beijing Nova Program(No.Z131107000413016)Project of Science and Technology Activities Preferred Overseas Personnel of Beijing(No.2014)
文摘Objective: To investigate the potential antifibrotic mechanisms of Chinese medicine Ganshuang Granules(肝爽颗粒, GSG) and to provide clinical therapeutic evidence of its effects. Methods: A cirrhotic mouse model was established by intraperitoneally injecting a mixture of CCl_4(40%) and oil(60%) at 0.2 mL per 100 g of body weight twice a week for 12 weeks. After 12-week modeling, GSG was intragastric administrated to the mice for 2 weeks, and the mice were divided into low-, medium-and high-dose groups at doses of 1, 2 and 4 g/(kg·day), respectively. Liver morphology changes were observed using Masson's trichrome staining and B-ultrasound. The levels of alanine aminotransferase(ALT), aspartate aminotransferase(AST) and hyaluronic acid(HA) in serum were detected using an automatic biochemistry analyzer. The expressions of desmin, smooth muscle actin(SMA) and Foxp3 in liver were detected by immunofluorescence. The regulatory T cell(Treg) frequency was determined through flow cytometry analysis. Collagen-Ⅰ, SMA, IL-6, tumor necrosis factor α(TNF-α), interleukin(IL)-1β and transforming growth factor β1(TGF-β1) expression levels were measured using quantitative polymerase chain reaction(qPCR). Results: Masson's staining result showed fewer pseudolobule structures and fibrous connective tissue in the GSG-treatment groups than in the spontaneous recovery group. Ultrasonography showed that GSG treatment reduced the number of punctate hyperechoic lesions in mice cirrhotic livers. The serum ALT, AST, HA levels were significantly ameliorated by GSG treatment(ALT: F=8.104, P=0.000; AST: F=7.078, P=0.002; and HA: F=7.621, P=0.001). The expression levels of collagen-Ⅰ and SMA in the cirrhotic livers were also attenuated by GSG treatment(collagen-Ⅰ: F=3.938, P=0.011; SMA: F=4.115, P=0.009). Tregs, which were elevated in the fibrotic livers, were suppressed by GSG treatment(F=8.268, P=0.001). The expressions of IL-6, TNF-α and IL-1β increased, and TGF-β levels decreased in the cirrhotic livers after GSG treatment(IL-6: F=5.457, P=0.004; TNF-α: F=6.023, P=0.002; IL-1β: F=6.658, P=0.001; and TGF-β1: F=11.239, P=0.000). Conclusions: GSG promoted the resolution/regression of cirrhosis and restored liver functions in part by suppressing Treg cell differentiation, which may be mediated by hepatic stellate cells.
文摘目的分析出生时维生素D水平与早产儿缺氧缺血性脑病(HIE)的相关性,为预防早产儿HIE提供依据。方法选择博罗县妇幼保健计划生育服务中心2018年6月—2020年6月诊断为HIE的早产儿25例(HIE组)和未发生HIE的早产儿138例(非HIE组),比较两组母亲和早产儿出生时血清25-(OH)D的水平,并对HIE的危险因素进行Logistic回归分析。结果与非HIE组比较,HIE组早产儿宫内窘迫发生率较高、1 min Apgar评分较低、机械通气时间较长,差异均有统计学意义(χ^(2)=46.875,t=10.854、7.166,P<0.01);HIE组母亲和早产儿出生时血清25-(OH)D水平明显低于非HIE组,差异有统计学意义(t=2.346、2.092,P<0.05),HIE组早产儿出生时维生素D缺乏或不足的比例(64%)明显高于非HIE组(39.9%),差异有统计学意义(χ^(2)=5.019,P<0.05);两组母亲血清25-(OH)D水平与早产儿出生时25-(OH)D水平呈正相关(HIE组r=0.825,非HIE组r=0.682,P<0.05)。Logistic回归分析结果显示HIE的危险因素包括宫内窘迫(OR=1.137,95%CI:1.105~1.209)、1 min低Apgar评分(OR=1.170,95%CI:1.085~1.443)、机械通气时间(OR=1.431,95%CI:1.275~1.610)和低25-(OH)D水平(母亲OR=1.234,95%CI:1.010~1.511;早产儿OR=1.505,95%CI:1.191~1.899)。结论早产儿出生时维生素D缺乏或不足的比例达50.9%(83/163),母亲及早产儿出生时低维生素D水平是早产儿HIE发生的高危因素,应注重孕期和早产儿出生后的维生素D补充。
