Diapause hormone (DH) is a neurohormone which is secreted from suboesophageal ganglion and responsible for induction of embryonic diapause in many insects. Using rapid amplification of the cDNA ends (RACE) method, the...Diapause hormone (DH) is a neurohormone which is secreted from suboesophageal ganglion and responsible for induction of embryonic diapause in many insects. Using rapid amplification of the cDNA ends (RACE) method, the cDNA encoding diapause hormone in Helicoverpa armigera, a main kind of crops pest was cloned. The nucleotide sequence reveals that the mRNA encodes an open reading frame and the 25-aa DH peptide is localized at N-terminal region just after the signal peptide. A homology search showed that H. armigera DH has high homology with the diapause hormone of Bornbyx mori and Helicoverpa zea; and it also belongs to the FXPRL neuropeptide family. Thus, H. armigera DH seems to be a new type of diapause hormone molecule.展开更多
文摘Diapause hormone (DH) is a neurohormone which is secreted from suboesophageal ganglion and responsible for induction of embryonic diapause in many insects. Using rapid amplification of the cDNA ends (RACE) method, the cDNA encoding diapause hormone in Helicoverpa armigera, a main kind of crops pest was cloned. The nucleotide sequence reveals that the mRNA encodes an open reading frame and the 25-aa DH peptide is localized at N-terminal region just after the signal peptide. A homology search showed that H. armigera DH has high homology with the diapause hormone of Bornbyx mori and Helicoverpa zea; and it also belongs to the FXPRL neuropeptide family. Thus, H. armigera DH seems to be a new type of diapause hormone molecule.