甘蓝型油菜转录因子BnaABI5表达特征分析及互作蛋白鉴定

甘蓝型油菜(Brassica napus L.)是重要的油料作物之一,但是其种植效益在西北地区经常受到干旱等环境逆境的影响。脱落酸(abscisic acid,ABA)信号通路在植物对非生物胁迫的响应与耐受中具有重要作用,而ABFs/AREBs(ABA-responsive element binding factors/ABA-responsive element binding proteins)是调控ABA响应基因表达的核心转录因子。为了解析油菜响应逆境的关键转录因子,本研究首先对甘蓝型油菜转录因子BnaABI5(abscisic acid insensitive 5)进行了亚细胞定位、逆境响应及组织器官表达的检测、转录活性分析以及与激酶BnaMPKs(mitogen-activated protein kinases)的互作筛选。结果显示:BnaABI5-GFP融合蛋白定位于细胞核,该基因响应干旱逆境且主要在油菜的根中表达,并且在酵母体系中呈现出一定的转录激活活性;随后利用烟草瞬时表达体系发现BnaABI5能够激活靶标基因EM6(early methionine-labeled 6)的启动子活性。通过双分子荧光互补(bimolecular fluorescence complementation,BiFC)及酵母双杂交(yeast two-hybrid,Y2H)实验,发现BnaABI5与BnaMPK6及BnaMPK13互作。本研究初步探索了转录因子BnaABI5的表达特征及与BnaMPKs的互作,对于深入理解BnaABI5的功能具有一定的指导意义。

Research on the Damping Mechanism of Time-Delay Coupled Negative Stiffness Dynamic Absorber in Nonlinear Vibration Damping System

A study was conducted on the effect of time delay and structural parameters on the vibration reduction of a time delayed coupled negative stiffness dynamic absorber in nonlinear vibration reduction systems. Taking dynamic absorbers with different structural and control parameters as examples, the effects of third-order nonlinear coefficients, time-delay control parameters, and negative stiffness coefficients on reducing the replication of the main system were discussed. The nonlinear dynamic absorber has a very good vibration reduction effect at the resonance point of the main system and a nearby area, and when 1 increases to a certain level, the stable region of the system continues to increase. The amplitude curve of the main system of a nonlinear dynamic absorber will generate Hop bifurcation and saddle node bifurcation in the region far from the resonance point, resulting in almost periodic motion and jumping phenomena in the system. For nonlinear dynamic absorbers with determined structural parameters, time-delay feedback control can be adopted to control the amplitude of the main system. For different negative stiffness coefficients, there exists a minimum damping point for the amplitude of the main system under the determined system structural parameters and time-delay feedback control parameters.

The Role and Safety of Personalized Care in Improving Thrombocytopenia after Lymphoma Chemotherapy

Objective:To explore and analyze the effect and safety of personalized nursing in improving thrombocytopenia after lymphoma chemotherapy.Methods:80 lymphoma patients with thrombocytopenia after lymphoma chemotherapy in the Department of Hematology of our hospital from May 2021 to May 2023 were selected as the research objects,and they were divided into an experimental group and reference group by random drawing,with 40 cases in each group.The experimental group received individualized nursing,and the reference group received routine nursing.The platelet count,platelet-related indicators,incidence of adverse reactions,and life scores were compared between the two groups.Results:Before the intervention,there was no significant difference in platelet count between the groups(P>0.05);after the intervention,the platelet count in the experimental group was significantly higher compared to the reference group(P<0.05).In the experimental group,the duration for platelet decline and the time for platelets to normalize were notably shorter compared to the reference group(P<0.05).Moreover,the personality group displayed a significantly lower incidence of adverse reactions than the reference group(P<0.05).Prior to the intervention,there were no statistically significant differences(P>0.05)in the life scores between the two groups,such as functional condition,symptom manifestation,and health scores among the groups.However,post-intervention,the personality group exhibited a significant improvement in those scores compared to the reference group(P<0.05).Conclusion:Individualized nursing can optimize the platelet level in thrombocytopenia after lymphoma chemotherapy,improve symptoms,and reduce the occurrence of adverse reactions.

5G ENABLED VEHICULAR COMMUNICATIONS AND NETWORKING

Vehicular communications and networking(VCN)is considered a billion-dollar industry.Its development not only possesses economic value,but also has enormous social potential.The earliest vehicular network started from the dedicated short range networks,such as the IEEE 802.11p standard established at the beginning of this century.In recent years,the public access network based VCN has been rapidly advanced.For example,LTE-V based on 4G LTE is under standardization。

Where Does AlphaGo Go: From Church-Turing Thesis to AlphaGo Thesis and Beyond

An investigation on the impact and significance of the AlphaGo vs. Lee Sedol Go match is conducted, and concludes with a conjecture of the AlphaGo Thesis and its extension in accordance with the Church-Turing Thesis in the history of computing. It is postulated that the architecture and method utilized by the AlphaGo program provide an engineering solution for tackling issues in complexity and intelligence. Specifically, the AlphaGo Thesis implies that any effective procedure for hard decision problems such as NP-hard can be implemented with AlphaGo-like approach. Deep rule-based networks are proposed in attempt to establish an understandable structure for deep neural networks in deep learning. The success of AlphaGo and corresponding thesis ensure the technical soundness of the parallel intelligence approach for intelligent control and management of complex systems and knowledge automation. © 2014 Chinese Association of Automation.

A Novel Forwarding Method for Full-Duplex Two-Way Relay Networks

An estimate-and-forward(EF) scheme for single-input single-output(SISO) and multiple-input multiple-output(MIMO) full-duplex two-way relay networks is proposed and analyzed. The relay estimates the received signal from two terminal nodes by a minimum mean squared error(MMSE) estimation and forwards a scaled version of the MMSE estimate to the destination. The proposed EF outperforms conventional amplify-and-forward(AF) and decode-and-forward(DF) across all signal-to-noise ratio(SNR) region. Because its computational complexity is high for relays with a large number of antennas(large MIMO) and/or high order constellations, an approximate EF scheme, called list EF, are thus proposed to reduce the computational complexity. The proposed list EF computes a candidate list for the MMSE estimate by using a sphere decoder, and it approaches the performance of the exact EF relay at a negligible performance loss. The proposed forwarding approach also could be used to other relay networks, such as half-duplex, one-way or massive MIMO relay networks.

Assessing anxiety,depression,and stress among inpatients with cancer

Objective This study aimed to assess anxiety,depression,and stress among inpatients with cancer.Methods Two hundred thirty-five hospitalized patients with cancer were surveyed with the Depression Anxiety Stress Scales(simplified Chinese Version).The software program SPSS 25.0 was used for statistical analysis of the survey data.Results The average scores of depression,anxiety,and stress of inpatients with cancer were 12.17,11.84,and 13.98 respectively,which were higher than the normal range.The scores of anxiety and stress of inpatients with different caregivers were statistically different(P=0.024/0.036).The anxiety and stress scores of inpatients with spouses as caregivers were higher than those of inpatients with children as caregivers.There was a statistically significant difference in the incidence of stress between inpatients with cancer with religious beliefs and inpatients with cancer without religious beliefs(P=0.026),and those with religious beliefs had greater incidence of stress.The score of anxiety was significantly higher for inpatients with children than for inpatients without children(P=0.040).Conclusion The anxiety,depression,and stress levels of inpatients with cancer are relatively high.It is necessary to pay special attention to the psychological status of these patients during clinical diagnosis and treatment to improve their quality of life.

Mental health status of cancer caregivers,assessment tools,and psychological interventions

Many studies pointed out that psychological pain is not limited to the cancer patients themselves,but their caregivers also experience different levels of psychological problems such as depression,anxiety,and stress.This article attempts to review the mental health status,assessment tools,and psychological interventions of the caregivers of cancer patients,and calls on social and medical workers to pay attention to the mental and physical health status of the caregivers of cancer patients.

Mental distress evaluation and intervention for cancer patients

Mental distress is prevalent among cancer patients.Many measurements have been developed to screen and evaluate such distress.About one-third of the persons with cancer will experience significant levels of distress,requiring targeted psychosocial intervention.Mental distress has been endorsed as the sixth vital sign by the International Psycho-Oncology Society(IPOS) in 2009.The need for effective screening and psychological interventions is well recognized as a necessary,integral part of oncology care.This systematic review examines the psychometric properties of the existing tools used to screen patients for emotional distress and the applicable intervention methods.

Process and Manufacturing of Ceramic Pattern and Its Digital Analysis by Computer

This paper compares Ceramic Decal design and traditional printing design through computer; show the innovation advantages of computer Ceramic Decal design prominent, such as computer color advantage, computer design software brings innovation advantage. The computer brings to the thinking innovation advantage and computer Ceramic Decal design than the traditional ceramic decal in the design process. Through the design advantage that computer has very important influence and significance of ceramic decal.

菜籽油和亚麻籽油对粒毛盘菌生物量和代谢产物的影响

本文主要研究了不同浓度的菜籽油和亚麻籽油对粒毛盘菌YM406生物量和代谢产物的影响。向培养基中分别添加0.5%、1%、2%、3%和4%的菜籽油和亚麻籽油进行发酵10天,发现当菜籽油浓度为1%,亚麻籽油浓度为2%时,生物量与未添加油脂的平均生物量(4.29 g/L)差距不大,分别是4.26 g/L和4.27 g/L,而其他浓度都会不同程度地抑制生物量。但同一油脂不同浓度间生物量没有显著性差异。不同浓度的菜籽油和亚麻籽油对胞外多糖(LEP)产量均有明显的抑制作用。选择添加1%菜籽油和添加2%亚麻籽油的发酵液,采用液相色谱-质谱联用技术(LC-MS),分别检测了经氯仿、乙酸乙酯萃取及未萃取的发酵液中代谢产物,检测结果显示添加1%菜籽油发酵液共检测出27种物质,添加2%亚麻籽油发酵液共检测出20种物质,添加这两种油脂的发酵液中检测出的物质只有4种相同,这说明不同类型的油脂对代谢产物类型有显著影响。添加1%菜籽油发酵液经两种萃取剂萃取后只有3种相同物质,而添加2%亚麻籽油后只有4种,说明不同萃取剂萃取后发酵液的检测结果差异明显。本研究结果表明,不同类型的油脂对粒毛盘菌YM406生物量和多糖产量具有抑制作用,并且不同浓度的油脂抑制作用不同;不同类型的油脂会影响粒毛盘菌YM406代谢产物种类。

LLM4CP:Adapting Large Language Models for Channel Prediction

Channel prediction is an effective approach for reducing the feedback or estimation overhead in massive multi-input multi-output (m-MIMO) systems. However, existing channel prediction methods lack precision due to model mismatch errors or network generalization issues. Large language models (LLMs) have demonstrated powerful modeling and generalization abilities, and have been successfully applied to cross-modal tasks, including the time series analysis. Leveraging the expressive power of LLMs, we propose a pre-trained LLM-empowered channel prediction(LLM4CP)method to predict the future downlink channel state information (CSI) sequence based on the historical uplink CSI sequence. We fine-tune the network while freezing most of the parameters of the pre-trained LLM for better cross-modality knowledge transfer. To bridge the gap between the channel data and the feature space of the LLM,preprocessor, embedding, and output modules are specifically tailored by taking into account unique channel characteristics. Simulations validate that the proposed method achieves state-of-the-art (SOTA) prediction performance on full-sample, few-shot, and generalization tests with low training and inference costs.

Isolation and characterization of mpox virus from the first mpox case in Shenzhen,China

Dear Editor,Unlike previous outbreaks of the mpox(previously known as monkeypox),which were localized in Africa and resulted in small numbers of infections due to limited human-to-human transmission,the current outbreak beginning in May 2022,has spread rapidly primarily through human-to-human transmission in non-endemic countries(Gong et al.,2022).As of 27 Sep 2023,115 countries and regions have reported cases of mpox to the World Health Organization(WHO),bringing the total number of confirmed cases to 90,618 including 125 deaths.Although the number of new cases has decreased in most WHO regions in the second half of 2022,the curves reveal a long outbreak tail in different subregions of the Americas.More worrying,106 mpox cases were reported in the mainland of China in June 2023.

Successful treatment of severe monkeypox case with advanced HIV infection using plasma from smallpoxvaccinated healthy population:A case report

Since the first reported case of monkeypox in the UK in May 2022,there has been an upward trend in monkeypox cases and a global outbreak.However,reports of severe cases are relatively limited.In this study,we report a case of severe monkeypox in a patient with HIV.The patient presented with skin lesions that started on his face and around the penis and persisted for several months.Throughout the course of the disease,he received systematic symp-tomatic supportive treatment,topical remedies,and special care for the rash.He also underwent cidofovir antiviral therapy and smallpox‐vaccinated healthy population‐derived plasma therapy in succession,with the condition ultimately showing improvement after plasma treatment.After more than 3 months of hospitalization,he fully recovered.To the best of our knowledge,this is the first reported use of smallpox‐vaccinated healthy population‐derived plasma in the treatment of severe monkeypox cases.

Cost-effectiveness of seasonal influenza vaccination of children in China:a modeling analysis

Background China has a high burden of influenza-associated illness among children.We aimed to evaluate the cost-effectiveness of introducing government-funded influenza vaccination to children in China(fully-funded policy)compared with the status quo(self-paid policy).Methods A decision tree model was developed to calculate the economic and health outcomes,from a societal perspective,using national-and provincial-level data.The incremental cost-effectiveness ratio(ICER)[incremental costs per quality-adjusted life year(QALY)gained]was used to compare the fully-funded policy with the self-paid policy under the willingness-to-pay threshold equivalent to national and provincial GDP per capita.Sensitivity analyses were performed and various scenarios were explored based on real-world conditions,including incorporating indirect effect into the analysis.Results Compared to the self-paid policy,implementation of a fully-funded policy could prevent 1,444,768[95%uncertainty range(UR):1,203,446-1,719,761]symptomatic cases,92,110(95%UR:66,953-122,226)influenza-related hospitalizations,and 6494(95%UR:4590-8962)influenza-related death per season.The fully-funded policy was cost-effective nationally(7964 USD per QALY gained)and provincially for 13 of 31 provincial-level administrative divisions(PLADs).The probability of a funded vaccination policy being cost-effective was 56.5%nationally,and the probability in 9 of 31 PLADs was above 75%.The result was most sensitive to the symptomatic influenza rate among children under 5 years[ICER ranging from−25,612(cost-saving)to 14,532 USD per QALY gained].The ICER of the fully-funded policy was substantially lower(becoming cost-saving)if the indirect effects of vaccination were considered.Conclusions Introducing a government-funded influenza policy for children is cost-effective in China nationally and in many PLADs.PLADs with high symptomatic influenza rate and influenza-associated mortality would benefit the most from a government-funded influenza vaccination program.

Insights into enhanced electrochemiluminescence of a multiresonance thermally activated delayed fluorescence molecule

The electrochemiluminescence(ECL)behavior of a multiresonance thermally activated delayed fluorescence molecule has been investigated for the first time by means of ECL‐voltage curves,newly designed ECL‐time observatory,and ECL spectroscopy.The compound,Mes3DiKTa,shows complex ECL behavior,including a delayed onset time of 5 ms for ECL generation in both the annihilation pathway and the coreactant route,which we attribute to organic long‐persistent ECL(OLECL).Triplet‐triplet annihilation,thermally activated delayed fluorescence and uncompensated solution resistance cannot be ruled out as contributing mechanisms to the ECL.A very long ECL emission decay was attributed to OLECL as well.The absolute ECL efficiencies of Mes3DiKTa were enhanced and reached 0.0013%in annihilation route and 1.1%for the coreactant system,which are superior to those of most other organic ECL materials.It is plausible that ECL materials with comparable behavior as Mes3DiKTa are desirable in applications such as ECL sensing,imaging,and light‐emitting devices.

Male marriage squeeze and a sociodemographic portrait of involuntary bachelors in rural China

This study uses,primarily,the 2020 National Population Census data to comprehensively examine the phenomenon of the“male marriage squeeze”and provide a socio-demographic portrait of involuntary bachelors in rural China.The descriptive findings make clear the pronounced male marriage squeeze in rural China.In 2020,China recorded its highest historical sex ratio of marriageable population,reaching 110.The age-specific proportions of never-married men surpass those of women,particularly in rural areas where a significant proportion of men remain unmarried throughout their lives.Between 2010 and 2020,men in rural areas exhibited a notable trend of delayed marriage.The likelihood of rural men getting married steadily declined during this decade,with rural men significantly less likely to get married than urban men.In China,the concentration of involuntary bachelors is mainly in rural areas,characterized by lower socioeconomic status,and inferior living conditions.While educational qualification among involuntary bachelors in rural areas has improved,it still lags behind that of currently or previously married men.Another problem is that the elderly population in rural areas faces resource scarcity,increasing the reliance on the minimum subsistence allowance.Elderly involuntary bachelors are generally in poorer health than their married peers,and the health disparity is widening.To make matters worse,a high percentage of these men live alone.

Adversarial attacks and defenses in physiological computing:a systematic review

Physiological computing uses human physiological data as system inputs in real time.It includes,or significantly overlaps with,brain-computer interfaces,affective computing,adaptive automation,health informatics,and physiological signal based biometrics.Physiological computing increases the communication bandwidth from the user to the computer,but is also subject to various types of adversarial attacks,in which the attacker deliberately manipulates the training and/or test examples to hijack the machine learning algorithm output,leading to possible user confusion,frustration,injury,or even death.However,the vulnerability of physiological computing systems has not been paid enough attention to,and there does not exist a comprehensive review on adversarial attacks to them.This study fills this gap,by providing a systematic review on the main research areas of physiological computing,different types of adversarial attacks and their applications to physiological computing,and the corresponding defense strategies.We hope this review will attract more research interests on the vulnerability of physiological computing systems,and more importantly,defense strategies to make them more secure.

心脏出生缺陷胎儿产前诊断与分级咨询后的随访研究

目的通过对心脏出生缺陷(CBD)胎儿进行准确产前诊断、分级咨询后, 结合胎儿CBD分级对其临床随访结果进行研究, 为产前-产后一体化治疗提供数据基础。方法收集2018年1月至2020年12月广东省人民医院产前诊断结果为CBD的胎儿1 971例的临床资料。按照预后、出生后可能的手术时机将胎儿CBD分成Ⅰ~Ⅵ级, 并提出分级标准及包含的常见疾病种类。随访孕妇经产前分级咨询后胎儿的结局, 计算引产率、活产率、产前产后超声诊断符合率等指标。统计各级的疾病种类构成比、不同分级胎儿的预后及一体化治疗的结局。结果本院产前诊断为CBD的胎儿1 971例, 占行胎儿心脏超声检查总人数的16.2%(1 971/12 188), 排除因其他原因引产者30例, 最终纳入本研究1 941例, 其中Ⅰ级196例(10.1%)、Ⅱ级433例(22.3%)、Ⅲ级615例(31.7%)、Ⅳ级261例(13.4%)、Ⅴ级388例(20.0%)、Ⅵ级48例(2.5%), Ⅱ级和Ⅲ级(手术时间为出生后1年内)共占54.0%(1 048/1 941)。部分病种的分布在不同分级中具有明显的占比优势, 具有代表性。1 747例成功随访的孕妇中因胎儿CBD终止妊娠者736例, 引产率为42.1%(736/1 747);死胎1例;活产1 010例, 活产率为57.8%(1 010/1 747), 无死产。活产儿中975例(96.5%, 975/1 010)产前与产后诊断一致, 漏诊3例, 误诊32例;Ⅰ~Ⅲ级(主要为严重、复杂先天性心脏病)活产儿产前与产后诊断符合率为98.5%(383/389)。258例胎儿已在出生后进行手术治疗, Ⅱ级和Ⅲ级的手术比例比较, 差异有统计学意义(χ2=47.3, P<0.001)。随着分级水平的升高, Ⅰ~Ⅴ级(除Ⅵ级外)的活产率逐渐上升, 引产率逐渐下降。经比较, Ⅰ~Ⅴ级之间引产率和活产率的差异均有统计学意义(χ2=623.6, P<0.001)。结论产前诊断和产前分级咨询是CBD胎儿产前-产后一体化治疗模式中的重要环节;根据胎儿CBD分级可以细化胎儿出生后的治疗策略, 指导分娩决策, 并可作为产前诊断及产前咨询开展是否规范合理的评价标准。

SIMO detection schemes for underwater optical wireless communication under turbulence

In underwater optical wireless communication(UOWC),a channel is characterized by abundant scattering/absorption effects and optical turbulence.Most previous studies on UOWC have been limited to scattering/absorption effects.However,experiments in the literature indicate that underwater optical turbulence(UOT)can cause severe degradation of UOWC performance.In this paper,we characterize an UOWC channel with both scattering/absorption and UOT taken into consideration,and a spatial diversity receiver scheme,say a singleinput–multiple-output(SIMO) scheme,based on a light-emitting-diode(LED) source and multiple detectors is proposed to mitigate deep fading.The Monte Carlo based statistical simulation method is introduced to evaluate the bit-error-rate performance of the system.It is shown that spatial diversity can effectively reduce channel fading and remarkably extend communication range.