Proteomic characterization of four subtypes of M2 macrophages derived from human THP-1 cells

Macrophages are widely distributed immune cells that contribute to tissue homeostasis.Human THP-1 cells have been widely used in various macrophage-associated studies,especially those involving pro-inflammatory M1 and anti-inflammatory M2 phenotypes.However,the molecular characterization of four M2 subtypes(M2a,M2b,M2c,and M2d)derived from THP-1has not been fully investigated.In this study,we systematically analyzed the protein expression profiles of human THP-1-derived macrophages(M0,M1,M2a,M2b,M2c,and M2d)using quantitative proteomics approaches.The commonly and specially regulated proteins of the four M2 subtypes and their potential biological functions were further investigated.The results showed that M2a and M2b,and M2c and M2d have very similar protein expression profiles.These data could serve as an important resource for studies of macrophages using THP-1 cells,and provide a reference to distinguish different M2 subtypes in macrophage-associated diseases for subsequent clinical research.

黄瓜苋科凝集素基因的表达分析与逆境调控

凝集素是一类具有特异性糖结合活性的蛋白质,通常具有1个或多个非催化的糖结合结构域。凝集素在植物对病原菌的防御反应中发挥重要作用。由于其抗细菌、真菌、病毒和昆虫等的活性,凝集素在农业和生物医药领域都具有很大的应用潜力。作为最小的凝集素家族之一,苋科凝集素的研究较少。该文通过对重要经济作物黄瓜(Cucumissativus)的基因组进行分析,对16种苋科凝集素基因在黄瓜基因组中的分布和位置进行研究,并分析相关基因的外显子/内含子组成。进一步通过启动子分析,阐明了苋科凝集素基因对非生物胁迫的响应情况。最后,通过实时荧光定量PCR,检测了黄瓜中4种苋科凝集素基因对低温、高盐、干旱和ABA处理的响应情况。研究结果可为揭示苋科凝集素的生理功能及其在植物胁迫响应中的作用提供参考。