Hepatitis B virus is a major liver disease caused by virus infection. Viral hepatitis is popular in China,mainly caused by hepatitis B. Experimental animal model is a necessary platform for the research on mechanism o...Hepatitis B virus is a major liver disease caused by virus infection. Viral hepatitis is popular in China,mainly caused by hepatitis B. Experimental animal model is a necessary platform for the research on mechanism of viral infection and pathogenicity,for treatment and vaccine development. Up to date,a great progress in the development of viral hepatitis animal models has been achieved in spite of the most of findings are limited to hepatitis B. Here,we summarized the recent findings of viral hepatitis animal models,focusing on the tree shrew animal model.展开更多
[Objectives] To study the effects of plant growth substance on the growth and development of Dendrobium officinale Kimura et Migo. [Methods] Comparative methods were applied to study the effects of plant growth substa...[Objectives] To study the effects of plant growth substance on the growth and development of Dendrobium officinale Kimura et Migo. [Methods] Comparative methods were applied to study the effects of plant growth substance on the growth and development of D. officinale Kimura et Migo. [Results] Plant growth regulators play an important role in the process of tissue culture rapid propagation of D. officinale Kimura et Migo,and the main regulatory pathways are as follows.( i) Plant growth regulators promote the growth and development of D. officinale Kimura et Migo through regulating the changes of its endogenous hormones.( ii) Plant growth regulators affect the accumulation of polysaccharide content in D. officinale Kimura et Migo through regulating the changes in chlorophyll content and enzymes involved in sucrose metabolism.( iii) Plant growth regulators can increase the activity of antioxidant enzymes of D. officinale Kimura et Migo,to increase its stress resistance. [Conclusions]Using molecular biology techniques,combined with the changes of plant hormones in D. officinale Kimura et Migo,and analysis on key enzyme in the synthesis of plant hormones and gene expression,it is feasible to study the rules o changes in the content of active content of D. officinale Kimura et Migo.展开更多
This study was conducted to investigate the changes of polysaccharides content in Dendrobii Officinalis Caulis and its pharmacological action. The results showed that different growth years, different periods, differe...This study was conducted to investigate the changes of polysaccharides content in Dendrobii Officinalis Caulis and its pharmacological action. The results showed that different growth years, different periods, different habitats and different external factors had influences on the change of polysaccharide content in Den- drobii Officinalis Caulis. Dendrobii Officinalis Caulis has certain effects of enhancing immunity, resisting fatigue and oxidation, promoting digestion and salivation secretion, reducing blood sugar and blood pressure, resisting tumor and liver injury, and relaxing bowels. Dendrobii Officinalis Caulis polysaccharides are the main effective components in Dendrobii Officinalis Caulis. The control of Dendrobii Officinalis Caulis polysaccharide content is of great significance to the assurance of the yield and quality of Dendrobii Officinalis Caulis material.展开更多
Research progress of pharmacognostical identification and chemical composition of Pholidota cantonensis Rolfe. was reviewed. This paper will facilitate assurance of the safety of clinical treatment.
[Objectives]The paper was to investigate the correlation between the microscopic characteristic constants and the index component polysaccharide content of Dendrobium officinale from Guangxi.[Methods]The paraffin sect...[Objectives]The paper was to investigate the correlation between the microscopic characteristic constants and the index component polysaccharide content of Dendrobium officinale from Guangxi.[Methods]The paraffin sections of D.officinale were made,and the microscopic characteristic constants of D.officinale stem,including cross section area,vascular bundle area and phloem area,were determined by NIS-Elements D microscopic image processing software.The contents of polysaccharides in D.officinale were determined by UV-Vis spectrophotometry,and the quantitative values of microscopic characteristics of D.officinale with different growth years were analyzed by SPSS 23.0(IBM SPSS Statistics 23.0)data statistical software.The correlation between quantitative microscopic characteristics of D.officinale and its polysaccharide content was analyzed.[Results]According to the correlation between the index component of D.officinale and its quantitative microscopic characteristics,the measurement indexes,the percentage of vascular bundle in the cross section area and the percentage of hard structure in the cross section area,were strongly negatively correlated with polysaccharide content.[Conclusions]The microscopic characteristic constant values of vascular bundles of D.officinale can be used as a method to verify polysaccharide content.展开更多
Traditional Chinese medicines are the material basis for the treatment of diseases under theoretical direction, as well as the raw materials of traditional Chinese medicine decoction pieces and Chinese patent medicine...Traditional Chinese medicines are the material basis for the treatment of diseases under theoretical direction, as well as the raw materials of traditional Chinese medicine decoction pieces and Chinese patent medicines. The quality of traditional Chinese medicine directly influences clinical efficacy. Therefore, the identification of traditional Chinese medicine is the responsibility of medicinal workers. The identification method develops from observation, tasting, smelling and listening to firing method and testing with water, and the construction of fingerprints of traditional Chinese medicines and molecular biological techniques. The development and evolution of traditional Chinese medicine identification methods was reviewed in this paper.展开更多
基金Supported by the Innovation Research Team Fund for Natural Science of Guangxi(2011GXNSFF018006)"2011 Collaborative Innovation Center"of Guangxi Province-Zhuang Yao Medicine Collaborative Innovation Center(GJKY[2013]20)+2 种基金Guangxi Key Discipline:Zhuang Pharmacy(GJKY[2013]16)Guangxi Key Laboratory of Zhuang Yao Medicine(GKJZ[2014]32)Chinese Traditional Medicine Innovation Theory and Drug Efficacy Study of Bagui Scholars
文摘Hepatitis B virus is a major liver disease caused by virus infection. Viral hepatitis is popular in China,mainly caused by hepatitis B. Experimental animal model is a necessary platform for the research on mechanism of viral infection and pathogenicity,for treatment and vaccine development. Up to date,a great progress in the development of viral hepatitis animal models has been achieved in spite of the most of findings are limited to hepatitis B. Here,we summarized the recent findings of viral hepatitis animal models,focusing on the tree shrew animal model.
基金Supported by Project of National Natural Science Foundation(81460587)Program of Collaborative Innovation Center for Zhuang Medicine of Guangxi(Gui Jiao Ke Yan[2013]20)+2 种基金Key Laboratory Project of Zhuang Medicine of Guangxi(Gui Ke Ji Zi[2014]32)Key Discipline Zhuang Medicine Program of Guangxi(Gui Jiao Ke Yan[2013]16)Eight Scholar Program of Guangxi"TCM Innovation Theory and Efficacy Research"
文摘[Objectives] To study the effects of plant growth substance on the growth and development of Dendrobium officinale Kimura et Migo. [Methods] Comparative methods were applied to study the effects of plant growth substance on the growth and development of D. officinale Kimura et Migo. [Results] Plant growth regulators play an important role in the process of tissue culture rapid propagation of D. officinale Kimura et Migo,and the main regulatory pathways are as follows.( i) Plant growth regulators promote the growth and development of D. officinale Kimura et Migo through regulating the changes of its endogenous hormones.( ii) Plant growth regulators affect the accumulation of polysaccharide content in D. officinale Kimura et Migo through regulating the changes in chlorophyll content and enzymes involved in sucrose metabolism.( iii) Plant growth regulators can increase the activity of antioxidant enzymes of D. officinale Kimura et Migo,to increase its stress resistance. [Conclusions]Using molecular biology techniques,combined with the changes of plant hormones in D. officinale Kimura et Migo,and analysis on key enzyme in the synthesis of plant hormones and gene expression,it is feasible to study the rules o changes in the content of active content of D. officinale Kimura et Migo.
基金Supported by National Natural Science Foundation of China(81460587)Zhuang Yao Medicine Collaborative InnovationC enter Project(GJKY[2013]20)+2 种基金Guangxi Key Laboratory of Zhuang Yao Medicine(GKJZ[2014]32)Guangxi Key Discipline:Zhuang Pharmacy(GJKY[2013]16)Chinese Traditional Medicine Innovation Theory and Drug EfficacyS tudy of Bagui Scholars
文摘This study was conducted to investigate the changes of polysaccharides content in Dendrobii Officinalis Caulis and its pharmacological action. The results showed that different growth years, different periods, different habitats and different external factors had influences on the change of polysaccharide content in Den- drobii Officinalis Caulis. Dendrobii Officinalis Caulis has certain effects of enhancing immunity, resisting fatigue and oxidation, promoting digestion and salivation secretion, reducing blood sugar and blood pressure, resisting tumor and liver injury, and relaxing bowels. Dendrobii Officinalis Caulis polysaccharides are the main effective components in Dendrobii Officinalis Caulis. The control of Dendrobii Officinalis Caulis polysaccharide content is of great significance to the assurance of the yield and quality of Dendrobii Officinalis Caulis material.
基金Supported by Zhuang Yao Medicine Collaborative Innovation Center(GJKY[2013]20)Guangxi Key Laboratory of Zhuang Yao Medicine(GKJZ[2014]32)+1 种基金Guangxi Key Discipline:Zhuang pharmacy(GJKY[2013]16)Chinese Traditional Medicine Innovation Theory and Drug Efficacy Study of Bagui Scholars(J13162)
文摘Research progress of pharmacognostical identification and chemical composition of Pholidota cantonensis Rolfe. was reviewed. This paper will facilitate assurance of the safety of clinical treatment.
基金Supported by 2020 Operation Subsidy Project of Guangxi Key Research Laboratory of Zhuang&Yao Medicine(20-065-14)"Quantification of Microscopic Characteristics of Dendrobium officinale from Guangxi and Its Correlation with the Quality of Medicinal Materials"(GXZYYKF2020A-13)2011 Guangxi Collaborative Innovation Center"Pharmaceutical Collaborative Innovation Center of Zhuang&Yao Medicine"(GJKY[2013]20)+6 种基金Guangxi Engineering Research Center for Resources and Application of Ethnic Medicine(GFGGJH[2020]2605)National Natural Science Foundation of China(82060695)Joint Research Laboratory of Zhuang&Yao Medicine of Guangxi University of Traditional Chinese Medicine and Central South University(GKJZ[2021]238)Guangxi Science and Technology Base and Talent Project(GK AD19245090)2018 Research Start-up Fund Project of Guangxi University of Chinese Medicine for Introduced Doctors(2018BS019)2019 Basic Scientific Research Ability Improvement Project for Young and Middle-aged Teachers in Colleges and Universities in Guangxi(2019KY0310)2019-2021 Guangxi First-class Discipline Construction Open Project of Guangxi University of Traditional Chinese Medicine(2019XK101)。
文摘[Objectives]The paper was to investigate the correlation between the microscopic characteristic constants and the index component polysaccharide content of Dendrobium officinale from Guangxi.[Methods]The paraffin sections of D.officinale were made,and the microscopic characteristic constants of D.officinale stem,including cross section area,vascular bundle area and phloem area,were determined by NIS-Elements D microscopic image processing software.The contents of polysaccharides in D.officinale were determined by UV-Vis spectrophotometry,and the quantitative values of microscopic characteristics of D.officinale with different growth years were analyzed by SPSS 23.0(IBM SPSS Statistics 23.0)data statistical software.The correlation between quantitative microscopic characteristics of D.officinale and its polysaccharide content was analyzed.[Results]According to the correlation between the index component of D.officinale and its quantitative microscopic characteristics,the measurement indexes,the percentage of vascular bundle in the cross section area and the percentage of hard structure in the cross section area,were strongly negatively correlated with polysaccharide content.[Conclusions]The microscopic characteristic constant values of vascular bundles of D.officinale can be used as a method to verify polysaccharide content.
基金Supported by Zhuang Yao Medicine Collaborative Innovation Center(GJKY[2013]20)Guangxi Key Laboratory of Zhuang Yao Medicine(GKJZ[2014]32)+2 种基金Guangxi Key Discipline:Zhuang Pharmacy(GJKY[2013]16)Chinese Traditional Medicine Innovation Theory and Drug Efficacy Study of Bagui Scholars(J13162)National Natural Science Foundation of China(81260620)
文摘Traditional Chinese medicines are the material basis for the treatment of diseases under theoretical direction, as well as the raw materials of traditional Chinese medicine decoction pieces and Chinese patent medicines. The quality of traditional Chinese medicine directly influences clinical efficacy. Therefore, the identification of traditional Chinese medicine is the responsibility of medicinal workers. The identification method develops from observation, tasting, smelling and listening to firing method and testing with water, and the construction of fingerprints of traditional Chinese medicines and molecular biological techniques. The development and evolution of traditional Chinese medicine identification methods was reviewed in this paper.