归芩牙膏中阿魏酸、黄芩苷含量测定及其药效学研究

建立高效液相色谱(HPLC)测定归芩牙膏中阿魏酸、黄芩苷含量的方法,研究其抗炎、镇痛、止血等药理作用。通过HPLC对归芩牙膏中君药当归、黄芩的主要成分阿魏酸、黄芩苷进行含量测定,色谱柱为Agilent ZOBAX SB C18(2.1 mm×150 mm,5μm),流动相为乙腈-0.2%乙酸水溶液(梯度洗脱),流速为1.0 mL/min,检测波长为320 nm,柱温为35℃。通过建立二甲苯致小鼠耳肿胀模型、小鼠热板法和小鼠肝脏创面出血模型考察归芩牙膏抗炎、镇痛及止血作用。并通过ELISA法检测小鼠耳廓组织中肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)、白介素-6(IL-6)等炎症因子的表达情况。阿魏酸、黄芩苷进样量分别在3.04~304μg/mL,2.86~286μg/mL范围内进样质量浓度与峰面积均呈现良好的线性关系(R^(2)>0.9997);精密度、稳定性、重复性试验RSD均小于3.0%;平均加样回收率分别为98.95%,99.87%,RSD分别为1.51%,0.22%。样品中每1 g含阿魏酸应不低于0.54 mg,含黄芩苷应不低于16.8 mg。HPLC法专属性强,重复性和稳定性良好,可用于归芩牙膏的质量控制。药效学实验表明归芩牙膏具有良好的抗炎、镇痛和止血作用。本研究为后期归芩牙膏的开发及临床应用提供了实验依据。

Effective therapy of the small-molecule cocktail 5SM on adult rat heart after ischemia–reperfusion injury

Dear Editor,Myocardial infarction(MI)is one of the leading causes of cardiovascular-related mortality worldwide.Timely restoration of the blood supply to ischemic my-ocardium by thrombolysis or percuta-neous coronary intervention is a com-mon clinical practice and decreases the mortality risk for MI patients(Heusch,2020).

Molecular regulation of myocardial proliferation and regeneration

Heart regeneration is a fascinating and complex biological process. Decades of intensive studies have revealed asophisticated molecular network regulating cardiac regeneration in the zebrafish and neonatal mouse heart. Here,we review both the classical and recent literature on the molecular and cellular mechanisms underlying heartregeneration, with a particular focus on how injury triggers the cell-cycle re-entry of quiescent cardiomyocytes toreplenish their massive loss after myocardial infarction or ventricular resection. We highlight several importantsignaling pathways for cardiomyocyte proliferation and propose a working model of how these injury-inducedsignals promote cardiomyocyte proliferation. Thus, this concise review provides up-to-date research progresses onheart regeneration for investigators in the field of regeneration biology.

Critical role of zebrafish dnajb5 in myocardial proliferation and regeneration

Myocardial infarction is a devastating disease worldwide.At present,nearly 40 million patients suffer from heart failure.Owing to a lack of adequate blood supply,25%of cardiomyocytes are subjected to apoptosis and necrosis within a few hours after infarction.It remains challenging to find effective therapeutic methods for heart failure.On the other hand,zebrafish and neonatal mouse hearts have a strong ability to regenerate(Poss et al.,2002;Raya et al.,2003;Porrello et al.,2011),and their regeneration derives from existing cardiomyocytes(Jopling et al.,2010;Kikuchi et al..

Research on the impact of mega-projects on carrying capacity of cities taking the first-line project of the West-East gas pipeline as an example

With the acceleration of China's urbanization,the functions and benefits of mega-projects are required higher.With the deepening of"the Silk Road Economic Belt and the 21st-Century Maritime Silk Road",the quantitative evaluation of multinational investment projects will help strengthen cross-border communication among gov-ernment,society and enterprises,which provide a basis for mutual trust.This study empirically analyzes and evaluates the impact of mega-projects on carrying capacity of cities,by using the DID method based on the panel data of 15 cities in China from 2000 to 2013.The results show that the construction and operation of the first-line project of the West-East gas pipeline has a significant positive impact on the improvement of carrying capacity of cities along the line.However,the degree of impact on three di-mensions is slightly different.The impact on the social carrying capacity is the greatest,then the ecological and economic carrying capacity.Therefore,the decisions of do-mestic mega-projects should be unified with urban planning to promote the sustain-able development of China's economy and social.The investment of mega-projects abroad should also be coordinated with the carrying capacity of host cities,in order to enhance the competitiveness and creativity of Chinese multinational mega-projects investment.

Cohort study of use of the hands-and knees-position as the first approach to resolving shoulder dystocia and preventing neonatal birth trauma

Background:McRoberts'maneuver,turning the woman's thighs up in the supine position,has become the dominant method for resolving shoulder dystocia(defined as failure to deliver the fetal shoulder(s)with gentle downward traction on the fetal head,requiring additional obstetric maneuvers to effect delivery).Another method that is less commonly used is the Gaskin maneuver,which was first described by the midwife Ina May Gaskin,who learned of it from traditional midwives in Central America,and involves turning women to the hands-and-knees position.One retrospective observational study reported an 84%higher resolution of shoulder dystocia and less injury to the baby with the use of the hands-and-knees position as the first approach to resolving shoulder dystocia.The hands-and-knees position was reported to result in a wider pelvic diameter than the supine position and hence facilitate rotation of the baby and delivery,so it is possibly to resolving shoulder dystocia.The purpose of this study is to explore whether applying the Gaskin maneuver as the first approach for resolving cases of shoulder dystocia is a safer and better method than applying the McRoberts'maneuver first.Methods:A time series cohort study was conducted in Laiwu Maternal and Child Health Hospital in Shandong,China over a seven-year period.Between January 2011 and July 2013 all women receiving traditional support at delivery were enrolled as a control group.During this time period,when shoulder dystocia was suspected,McRoberts’maneuver(MR)was the most commonly employed first maneuver as described in the HELPERR mnemonics.Between October 2013 and December 2017,a change of practice was implemented for all vaginal births at the site,whereby midwives were instructed to use the Gaskin maneuver(i.e.,moving the mother onto hands-and-knees position)as the first protocol if shoulder dystocia was suspected.Patients in this group were assigned to the experimental group.The neonatal outcomes including birth asphyxia and baby injury in both groups were recorded and analyzed.Results:The injuries in the control group included 14 clavicular fractures,and one permanent brachial plexus injury,three temporary arm movement disorders that resolved within 7 days and those cases were not calculated as baby injury cases(the same in experimental group).In the experimental group,58 cases of shoulder dystocia were reported,with one case of clavicular fracture,three temporary arm movement disorders that resolved within 3 days,and no permanent brachial plexus injuries.The rate of baby injury in the control group[14.6%(15/103)]was higher than that in the experimental group[1.7%(1/58)],and this difference was statistically significant[χ^(2)=6.834,P=0.009;OR=1.150(1.055–1.254)].Multiple logistic regression analysis showed that using the McRoberts'maneuver as the first approach in cases of shoulder dystocia[OR=19.609(1.620–273.430)],the mother's employment status[OR=1.909(1.118–3.262)],and intravenous dripping of oxytocin[OR=5.969(1.391–25.605)]are risk factors for baby injury.There was no difference in neonate Apgar score between the two groups,and no baby died in either group.