BACKGROUND: Serotonin transporter (5-HTT) polymorphisms comprise 5-HTT gene-linked polymorphism region (5-HTTLPR) and variable number of tandem repeats (VNTR). Studies have revealed an association between 5-HTT...BACKGROUND: Serotonin transporter (5-HTT) polymorphisms comprise 5-HTT gene-linked polymorphism region (5-HTTLPR) and variable number of tandem repeats (VNTR). Studies have revealed an association between 5-HTT polymorphism and major depressive disorder, which suggests that the "S" allele of 5-HTTLPR and Stin2.9 of 5-HTTVNTR are associated with major depressive disorder. However, there are a number of studies that do not support the 5-HTT polymorphism effect in major depressive disorder. OBJECTIVE: To study the relationship between 5-HTT gene polymorphism and major depressive disorder in Chinese Han population. DESIGN, TIME AND SETTING: Case-controlled study of 5-HTT gene polymorphism. The experiment was performed at the Central Laboratory, Third Affiliated Hospital of Sun Yat-sen University, China from March 2005 to January 2006. PARTICIPANTS: A total of 99 depressive patients of Chinese Han nationality were recruited for this study. All patients met DSM-IV diagnostic criteria for major depressive disorder and had a total score of Hamilton Depression Scale (24 items) ≥21 points. In addition, 101 healthy subjects, matched for age and gender, served as the control group. METHODS: Venous blood was collected from all subjects. 5-HTT genotypes and alleles were determined by polymerase chain reaction. Consistent with the Hardy-Weinberg equilibrium, the association between 5-HTT gene polymorphism and major depressive disorder were analyzed by Chi-square test. MAIN OUTCOME MEASURES: 5-HTTLPR and 5-HTTVNTR genotypes and allele frequencies were measured. RESULTS: No significant differences in 5-HTTLPR genotypes and allele frequencies were determined between patients and controls (P 〉 0.05). However, significant differences in 5-HTTVNTR genotypes and allele frequencies were detected (P 〈 0.01 ). The Stin2.10 allele and 10/10 genotype associated with major depressive disorder (OR = 2.61,7.7, P 〈 0.05; analysis of dose-response relationships Х^2 = 12.35, P 〈 0.01). CONCLUSION: Results from the present study revealed no association between 5-HTTLPR and major depressive disorder. However, a significant association between 5-HTTVNTR and major depressive disorder existed in a population of Chinese Han. The presence of Stin2.10 and 10/10 genotypes increased the risk for major depressive disorder in a dose-dependent manner.展开更多
Background:Since RNA sequencing has shown that induced pluripotent stem cells(iPSCs)share a common antigen profile with tumor cells,cancer vaccines that focus on iPSCs have made promising progress in recent years.Prev...Background:Since RNA sequencing has shown that induced pluripotent stem cells(iPSCs)share a common antigen profile with tumor cells,cancer vaccines that focus on iPSCs have made promising progress in recent years.Previously,we showed that iPSCs derived from leukemic cells of patients with primary T cell acute lymphoblastic leukemia(T-ALL)have a gene expression profile similar to that of T-ALL cell lines.Methods:Mice with T-ALL were treated with dendritic and T(DC-T)cells loaded with intact and complete antigens from T-ALL-derived iPSCs(T-ALL-iPSCs).We evaluated the safety and antitumor efficiency of autologous tumor-derived iPSC antigens by flow cytometry,cytokine release assay,acute toxicity experiments,long-term toxicity experiments,and other methods.Results:Our results indicate that complete tumor antigens from T-ALL-iPSCs could inhibit the growth of inoculated tumors in immunocompromised mice without causing acute and long-term toxicity.Conclusion:T-ALL-iPSC-based treatment is safe and can be used as a potential strategy for leukemia immunotherapy.展开更多
Background:The relationship between the regression and prognosis of melanoma has been debated for years.When competing-risk events are present,using traditional survival analysis methods may induce bias in the identif...Background:The relationship between the regression and prognosis of melanoma has been debated for years.When competing-risk events are present,using traditional survival analysis methods may induce bias in the identified prognostic factors that affect patients with regressive melanoma.Methods:Data on patients diagnosed with regressive melanoma were extracted from the Surveillance,Epidemiology,and End Results(SEER)database during 2000-2019.Cumulative incidence function and Gray's test were used for the univariate analysis,and the Cox proportional-hazards model and the Fine-Gray model were used for the multivariate analysis.Results:A total of 1442 eligible patients were diagnosed with regressive melanoma,including 529 patients who died:109 from regressive melanoma and 420 from other causes.The multivariate analysis using the Fine-Gray model revealed that SEER stage,surgery status,and marital status were important factors that affected the prognosis of regressive melanoma.Due to the existence of competing-risk events,the Cox model may have induced biases in estimating the effect values,and the competing-risks model was more advantageous in the analysis of multipleendpoint clinical survival data.Conclusion:The findings of this study may help clinicians to better understand regressive melanoma and provide reference data for clinical decisions.展开更多
基金a grant from the Foundation of Guangdong Province of Science and Technology,No. 2003C3380
文摘BACKGROUND: Serotonin transporter (5-HTT) polymorphisms comprise 5-HTT gene-linked polymorphism region (5-HTTLPR) and variable number of tandem repeats (VNTR). Studies have revealed an association between 5-HTT polymorphism and major depressive disorder, which suggests that the "S" allele of 5-HTTLPR and Stin2.9 of 5-HTTVNTR are associated with major depressive disorder. However, there are a number of studies that do not support the 5-HTT polymorphism effect in major depressive disorder. OBJECTIVE: To study the relationship between 5-HTT gene polymorphism and major depressive disorder in Chinese Han population. DESIGN, TIME AND SETTING: Case-controlled study of 5-HTT gene polymorphism. The experiment was performed at the Central Laboratory, Third Affiliated Hospital of Sun Yat-sen University, China from March 2005 to January 2006. PARTICIPANTS: A total of 99 depressive patients of Chinese Han nationality were recruited for this study. All patients met DSM-IV diagnostic criteria for major depressive disorder and had a total score of Hamilton Depression Scale (24 items) ≥21 points. In addition, 101 healthy subjects, matched for age and gender, served as the control group. METHODS: Venous blood was collected from all subjects. 5-HTT genotypes and alleles were determined by polymerase chain reaction. Consistent with the Hardy-Weinberg equilibrium, the association between 5-HTT gene polymorphism and major depressive disorder were analyzed by Chi-square test. MAIN OUTCOME MEASURES: 5-HTTLPR and 5-HTTVNTR genotypes and allele frequencies were measured. RESULTS: No significant differences in 5-HTTLPR genotypes and allele frequencies were determined between patients and controls (P 〉 0.05). However, significant differences in 5-HTTVNTR genotypes and allele frequencies were detected (P 〈 0.01 ). The Stin2.10 allele and 10/10 genotype associated with major depressive disorder (OR = 2.61,7.7, P 〈 0.05; analysis of dose-response relationships Х^2 = 12.35, P 〈 0.01). CONCLUSION: Results from the present study revealed no association between 5-HTTLPR and major depressive disorder. However, a significant association between 5-HTTVNTR and major depressive disorder existed in a population of Chinese Han. The presence of Stin2.10 and 10/10 genotypes increased the risk for major depressive disorder in a dose-dependent manner.
基金Science,Technology and Innovation Commission of Shenzhen Municipality,Grant/Award Number:CYJ20170412155231633Health Commission of Shenzhen Municipality,Grant/Award Number:SZXK062。
文摘Background:Since RNA sequencing has shown that induced pluripotent stem cells(iPSCs)share a common antigen profile with tumor cells,cancer vaccines that focus on iPSCs have made promising progress in recent years.Previously,we showed that iPSCs derived from leukemic cells of patients with primary T cell acute lymphoblastic leukemia(T-ALL)have a gene expression profile similar to that of T-ALL cell lines.Methods:Mice with T-ALL were treated with dendritic and T(DC-T)cells loaded with intact and complete antigens from T-ALL-derived iPSCs(T-ALL-iPSCs).We evaluated the safety and antitumor efficiency of autologous tumor-derived iPSC antigens by flow cytometry,cytokine release assay,acute toxicity experiments,long-term toxicity experiments,and other methods.Results:Our results indicate that complete tumor antigens from T-ALL-iPSCs could inhibit the growth of inoculated tumors in immunocompromised mice without causing acute and long-term toxicity.Conclusion:T-ALL-iPSC-based treatment is safe and can be used as a potential strategy for leukemia immunotherapy.
基金Key Scientific Problems and Medical Technical Problems Research Project of China Medical Education Association(2022KTZ009)Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Informatization(2021B1212040007).
文摘Background:The relationship between the regression and prognosis of melanoma has been debated for years.When competing-risk events are present,using traditional survival analysis methods may induce bias in the identified prognostic factors that affect patients with regressive melanoma.Methods:Data on patients diagnosed with regressive melanoma were extracted from the Surveillance,Epidemiology,and End Results(SEER)database during 2000-2019.Cumulative incidence function and Gray's test were used for the univariate analysis,and the Cox proportional-hazards model and the Fine-Gray model were used for the multivariate analysis.Results:A total of 1442 eligible patients were diagnosed with regressive melanoma,including 529 patients who died:109 from regressive melanoma and 420 from other causes.The multivariate analysis using the Fine-Gray model revealed that SEER stage,surgery status,and marital status were important factors that affected the prognosis of regressive melanoma.Due to the existence of competing-risk events,the Cox model may have induced biases in estimating the effect values,and the competing-risks model was more advantageous in the analysis of multipleendpoint clinical survival data.Conclusion:The findings of this study may help clinicians to better understand regressive melanoma and provide reference data for clinical decisions.