血清自身抗体标志物具有标本易获取、检测技术简便、可实现实时动态监测等优势。随着免疫检查点抑制剂在肿瘤治疗中的广泛应用,自身抗体标志物在肿瘤免疫检查点抑制剂治疗疗效、预后及免疫相关不良事件(immune related adverse events,i...血清自身抗体标志物具有标本易获取、检测技术简便、可实现实时动态监测等优势。随着免疫检查点抑制剂在肿瘤治疗中的广泛应用,自身抗体标志物在肿瘤免疫检查点抑制剂治疗疗效、预后及免疫相关不良事件(immune related adverse events,irAEs)发生预测方面的报道逐渐增多,显示出了良好的预测潜力。本文主要探讨自身抗体标志物在肿瘤免疫治疗疗效、预后及irAE预测方面的研究进展,并对未来前景做出展望。展开更多
To lower the retrogradation and digestibility of waxy corn starch for different food applications,a novel thermostable GtfC type 4,6-α-glucanotransferase without N-and C-terminals(GsGtfC)from Geobacillus sp.12AMOR1 w...To lower the retrogradation and digestibility of waxy corn starch for different food applications,a novel thermostable GtfC type 4,6-α-glucanotransferase without N-and C-terminals(GsGtfC)from Geobacillus sp.12AMOR1 was used.Waxy corn starch of 50 g/L was incubated with GsGtfC of 40-100 U/g substrate at 65℃and pH 5.5 for 1 h.Its molecular weight,iodine affinity,XRD crystallinity,and FTIR ratio of heights of bands at 1047 and 1022 cm^(-1) decreased,but ratio of DP<6 to DP≥25 branches and degree of branching increased.GsGtfC cleavedα-1,4-glycosidic bonds and inducedα-1,6-branching points to produce reuteran-likes polymers,which is different from Exiguobacterium sibiricum GtfC enzyme cleavingα-1,4-glycosidic bonds and synthesizing consecutiveα-1,6-glycosidic bonds to produce isomalto/malto-oligosaccharides.GsGtfC modified waxy corn starch had significantly lower DSC retrogradation enthalpies during the storage at 4℃for 3-14 days and significantly lower released glucose during the incubation with mammalian mucosalα-glucosidase at 37℃for 10-360 min.GsGtfC at 100 U/g substrate increased slowly digestible portion from 11.07%to 24.11%.展开更多
文摘血清自身抗体标志物具有标本易获取、检测技术简便、可实现实时动态监测等优势。随着免疫检查点抑制剂在肿瘤治疗中的广泛应用,自身抗体标志物在肿瘤免疫检查点抑制剂治疗疗效、预后及免疫相关不良事件(immune related adverse events,irAEs)发生预测方面的报道逐渐增多,显示出了良好的预测潜力。本文主要探讨自身抗体标志物在肿瘤免疫治疗疗效、预后及irAE预测方面的研究进展,并对未来前景做出展望。
基金This work was sponsored in part by National Natural Science Foundation of China(NSFC-31671801,31371749)the key R&D program(21ZGN38)from Changchun science and technology bureau,the special project of industrial independent innovation capability(2020C036-7)from Jilin provincial development and reform commission+1 种基金the science and technology research planning project(JJKH20220609KJ)from Jilin provincial department of educationthe scholar climbing project(ZKP202006 and ZKP202016)from Changchun University.
文摘To lower the retrogradation and digestibility of waxy corn starch for different food applications,a novel thermostable GtfC type 4,6-α-glucanotransferase without N-and C-terminals(GsGtfC)from Geobacillus sp.12AMOR1 was used.Waxy corn starch of 50 g/L was incubated with GsGtfC of 40-100 U/g substrate at 65℃and pH 5.5 for 1 h.Its molecular weight,iodine affinity,XRD crystallinity,and FTIR ratio of heights of bands at 1047 and 1022 cm^(-1) decreased,but ratio of DP<6 to DP≥25 branches and degree of branching increased.GsGtfC cleavedα-1,4-glycosidic bonds and inducedα-1,6-branching points to produce reuteran-likes polymers,which is different from Exiguobacterium sibiricum GtfC enzyme cleavingα-1,4-glycosidic bonds and synthesizing consecutiveα-1,6-glycosidic bonds to produce isomalto/malto-oligosaccharides.GsGtfC modified waxy corn starch had significantly lower DSC retrogradation enthalpies during the storage at 4℃for 3-14 days and significantly lower released glucose during the incubation with mammalian mucosalα-glucosidase at 37℃for 10-360 min.GsGtfC at 100 U/g substrate increased slowly digestible portion from 11.07%to 24.11%.
