Background and Aims:Hepatocellular carcinoma(HCC)is a common and deadly cancer.Accumulating evidence supports modulation of autophagy as a novel approach for determining cancer cell fate.The aim of this study to evalu...Background and Aims:Hepatocellular carcinoma(HCC)is a common and deadly cancer.Accumulating evidence supports modulation of autophagy as a novel approach for determining cancer cell fate.The aim of this study to evaluate the effectiveness of sarmentosin,a natural compound,on HCC in vitro and in vivo and elucidated the underlying mechanisms.Methods:Cell functions and signaling pathways were analyzed in HepG2 cells using western blotting,real-time PCR,siRNA,transmission electron microscopy and flow cytometry.BALB/c nude mice were injected with HepG2 cells to produce a xenograft tumour nude mouse model for in vivo assessments and their tumors,hearts,lungs and kidneys were isolated.Results:We found that autophagy was induced by sarmentosin in a concentration-and timedependent manner in human HCC HepG2 cells by western blot assays and scanning electron microscopy.Sarmentosin-induced autophagy was abolished by the autophagy inhibitors 3-methyladenine,chloroquine,and bafilomycin A1.Sarmentosin activated Nrf2 in HepG2 cells,as shown by increased nuclear translocation and upregulated expression of Nrf2 target genes.Phosphorylation of mTOR was also inhibited by sarmentosin.Sarmentosin stimulated caspasedependent apoptosis in HepG2 cells,which was impaired by silencing Nrf2 or chloroquine or knocking down ATG7.Finally,sarmentosin effectively repressed HCC growth in xenograft nude mice and activated autophagy and apoptosis in HCC tissues.Conclusions:This study showed sarmentosin stimulated autophagic and caspase-dependent apoptosis in HCC,which required activation of Nrf2 and inhibition of mTOR.Our research supports Nrf2 as a therapeutic target for HCC and sarmentosin as a promising candidate for HCC chemotherapy.展开更多
The novel coronavirus(COVID-19)that has been spreading worldwide since December 2019 has sickened millions of people,lock down major cities and some countries,prompted unprecedented global travel restrictions.Real dat...The novel coronavirus(COVID-19)that has been spreading worldwide since December 2019 has sickened millions of people,lock down major cities and some countries,prompted unprecedented global travel restrictions.Real data-driven modeling is an effort to help evaluate and curb the spread of the novel virus.Lockdowns and the effectiveness of reduction in the contacts in Italy has been measured via our modified model,with the addition of auxiliary and state variables that represent,contacts with infected,conversion rate and latent propagation.Results show the decrease in infected people due to stay-athome orders and tracing quarantine intervention.The effect of quarantine and centralized medical treatment was also measured through numerical modeling analysis.展开更多
基金This study was supported by grants from the financial support provided by Development Plan Project(SYSD2020221)the Fifth Batch of Suzhou Health Talents Project(GSWS2019075)the Science and Technology Support Program of Jiangsu Province(BE2009682).
文摘Background and Aims:Hepatocellular carcinoma(HCC)is a common and deadly cancer.Accumulating evidence supports modulation of autophagy as a novel approach for determining cancer cell fate.The aim of this study to evaluate the effectiveness of sarmentosin,a natural compound,on HCC in vitro and in vivo and elucidated the underlying mechanisms.Methods:Cell functions and signaling pathways were analyzed in HepG2 cells using western blotting,real-time PCR,siRNA,transmission electron microscopy and flow cytometry.BALB/c nude mice were injected with HepG2 cells to produce a xenograft tumour nude mouse model for in vivo assessments and their tumors,hearts,lungs and kidneys were isolated.Results:We found that autophagy was induced by sarmentosin in a concentration-and timedependent manner in human HCC HepG2 cells by western blot assays and scanning electron microscopy.Sarmentosin-induced autophagy was abolished by the autophagy inhibitors 3-methyladenine,chloroquine,and bafilomycin A1.Sarmentosin activated Nrf2 in HepG2 cells,as shown by increased nuclear translocation and upregulated expression of Nrf2 target genes.Phosphorylation of mTOR was also inhibited by sarmentosin.Sarmentosin stimulated caspasedependent apoptosis in HepG2 cells,which was impaired by silencing Nrf2 or chloroquine or knocking down ATG7.Finally,sarmentosin effectively repressed HCC growth in xenograft nude mice and activated autophagy and apoptosis in HCC tissues.Conclusions:This study showed sarmentosin stimulated autophagic and caspase-dependent apoptosis in HCC,which required activation of Nrf2 and inhibition of mTOR.Our research supports Nrf2 as a therapeutic target for HCC and sarmentosin as a promising candidate for HCC chemotherapy.
文摘The novel coronavirus(COVID-19)that has been spreading worldwide since December 2019 has sickened millions of people,lock down major cities and some countries,prompted unprecedented global travel restrictions.Real data-driven modeling is an effort to help evaluate and curb the spread of the novel virus.Lockdowns and the effectiveness of reduction in the contacts in Italy has been measured via our modified model,with the addition of auxiliary and state variables that represent,contacts with infected,conversion rate and latent propagation.Results show the decrease in infected people due to stay-athome orders and tracing quarantine intervention.The effect of quarantine and centralized medical treatment was also measured through numerical modeling analysis.
