[Objectives] This study was conducted to expand the insect resistance spectrum of tea saponin, and its control effect on Cylas formicarius and the potential as an insecticide for pest control were explored. [Methods] ...[Objectives] This study was conducted to expand the insect resistance spectrum of tea saponin, and its control effect on Cylas formicarius and the potential as an insecticide for pest control were explored. [Methods] The olfactory avoidance rate of C. formicarius to tea saponin aqueous solution was determined by Y-type olfactometer;the feeding avoidance rate of C. formicarius to tea saponin was determined by the selective method;the antifeedant rate of C. formicarius to tea saponin was determined by non-selective method;and the development duration and mortality of C. formicarius under the influence of tea saponin were determined by artificial feeding method. [Results] C. formicarius had no significant olfactory tendency to every concentration of tea saponin, and the olfactory avoidance rate of 20.0% tea saponin aqueous solution was only 9.14%. Tea saponin had a feeding avoidance effect on C. formicarius, and the avoidance rate increased with the increase of tea saponin concentration. At 6 h, the feeding avoidance rates of 0.5%, 1.0%, 5.0%, 10.0% and 20.0% tea saponin on C. formicarius were 58.14%, 77.77%, 88.23%, 95.00% and 97.65 %, respectively;and the feeding avoidance effect at 6 h was significant, and the feeding avoidance rate was higher than that of 1 h. Tea saponin had a significant antifeedant effect on C. formicarius, and the longer the feeding time, the higher the antifeedant rate. At 72 h, the antifeedant rates of 0.5%, 1.0%, 5.0%, 10.0 % and 20.0% tea saponin to C. formicarius were 63.01%, 67.54%, 97.14 %, 96.42% and 98.57%, respectively. The larval development duration of C. formicarius was shortened with the increase of tea saponin concentration, and the larval death occurred. The development duration of larvae under the influence of 1.0% tea saponin was the shortest, which was 4.01 d shorter than that of the control, and the mortality was the highest, which was 26.65%. [Conclusions] Tea saponin had neither olfactory avoidance effect nor olfactory attracting effect to C. formicarius, but had obvious feeding avoidance effect and strong antifeedant effect. Tea saponin can shorten the development duration of the larvae of C. formicarius and cause the death of the larvae.展开更多
基金Supported by National Modern Agricultural Industry Technology System Guangxi Industry Potato Innovation Team(nycytxgxcxtd-11-01)Guangxi Science and Technology Planning Project(GK AB16380046,GK AB18221101)
文摘[Objectives] This study was conducted to expand the insect resistance spectrum of tea saponin, and its control effect on Cylas formicarius and the potential as an insecticide for pest control were explored. [Methods] The olfactory avoidance rate of C. formicarius to tea saponin aqueous solution was determined by Y-type olfactometer;the feeding avoidance rate of C. formicarius to tea saponin was determined by the selective method;the antifeedant rate of C. formicarius to tea saponin was determined by non-selective method;and the development duration and mortality of C. formicarius under the influence of tea saponin were determined by artificial feeding method. [Results] C. formicarius had no significant olfactory tendency to every concentration of tea saponin, and the olfactory avoidance rate of 20.0% tea saponin aqueous solution was only 9.14%. Tea saponin had a feeding avoidance effect on C. formicarius, and the avoidance rate increased with the increase of tea saponin concentration. At 6 h, the feeding avoidance rates of 0.5%, 1.0%, 5.0%, 10.0% and 20.0% tea saponin on C. formicarius were 58.14%, 77.77%, 88.23%, 95.00% and 97.65 %, respectively;and the feeding avoidance effect at 6 h was significant, and the feeding avoidance rate was higher than that of 1 h. Tea saponin had a significant antifeedant effect on C. formicarius, and the longer the feeding time, the higher the antifeedant rate. At 72 h, the antifeedant rates of 0.5%, 1.0%, 5.0%, 10.0 % and 20.0% tea saponin to C. formicarius were 63.01%, 67.54%, 97.14 %, 96.42% and 98.57%, respectively. The larval development duration of C. formicarius was shortened with the increase of tea saponin concentration, and the larval death occurred. The development duration of larvae under the influence of 1.0% tea saponin was the shortest, which was 4.01 d shorter than that of the control, and the mortality was the highest, which was 26.65%. [Conclusions] Tea saponin had neither olfactory avoidance effect nor olfactory attracting effect to C. formicarius, but had obvious feeding avoidance effect and strong antifeedant effect. Tea saponin can shorten the development duration of the larvae of C. formicarius and cause the death of the larvae.
