Relatively poor in vitro rooting has limited the large-scale commercial production of tree peony.In this study,on the basis of transcriptome sequencing,differentially expressed genes and the associated metabolic pathw...Relatively poor in vitro rooting has limited the large-scale commercial production of tree peony.In this study,on the basis of transcriptome sequencing,differentially expressed genes and the associated metabolic pathways were identified in tree peony roots at different stages of root formation under sandy loam cultivation.A total of 31.63 Gb raw data were generated and 120,188 unigenes(mean length of 911.98 bp)were annotated according to six databases(NR,NT,GO,KEGG,COG,and Swiss-Prot).Analyses of the ungerminated root primordium period,induced root primordium period,and root formation period detected 8,232,6,907,and 10,687 differentially expressed genes related to 133,132,and 133 metabolic pathways,respectively.Two significantly differentially expressed genes(Unigene13430_All and CL10096.Contig1_All)were associated with the auxin pathway.The full-length Unigene13430_All coding sequence(843 bp)encoded 280 amino acids,whereas the full-length CL10096.Contig1_All coding sequence(1,470 bp)encoded 489 amino acids.Unigene13430_All and CL10096.Contig1_All were identified as IAA gene family members and were respectively named PsIAA27 and PsARF19.The qRT-PCR analysis and functional verification indicated that the expressions of PsARF19 and PsIAA27 in tree peony seedlings,cuttings and grafted seedlings were significant different.PsARF19 promoted root development,it might be a regulatory gene related to the formation of tree peony roots,while PsIAA27 inhibited lateral root development,and it might be involved in controlling auxin sensitivity during root formation.The results of this study may form the basis of future investigations on the mechanism mediating peony root formation.The transcriptome data will be an excellent resource for researchers interested in characterizing the rooting-related tree peony genes.展开更多
Meyoid derived suppressor cells(MDSCs)are heterogeneous myeloid cell populations that expand in pathological conditions such as cancer,autoimmunity,and infection;these cells are also known to exert strong immunosuppre...Meyoid derived suppressor cells(MDSCs)are heterogeneous myeloid cell populations that expand in pathological conditions such as cancer,autoimmunity,and infection;these cells are also known to exert strong immunosuppressive effects on primitive Tcell responses.1 MDSCs are derived from myeloid progenitors and are arrested at an immature phase of differentiation.In mice,normal bone marrow contains~20–30%cells exhibiting the MDSC phenotype.2 In contrast,a very low number of MDSCs are found in naive spleens(2–4%),and these cells are completely absent in lymph nodes.展开更多
基金financially supported by the National Key Research and Development Program of China(Grant No.2020YFD1000503)the Natural Science Foundation of China(Grant Nos.31870698,32001353)+1 种基金the Key Scientific and Technological Projects of Henan Province(Grant No.202102110083)the Science and Technology Program of Shanghai(Grant No.21DZ1202000).
文摘Relatively poor in vitro rooting has limited the large-scale commercial production of tree peony.In this study,on the basis of transcriptome sequencing,differentially expressed genes and the associated metabolic pathways were identified in tree peony roots at different stages of root formation under sandy loam cultivation.A total of 31.63 Gb raw data were generated and 120,188 unigenes(mean length of 911.98 bp)were annotated according to six databases(NR,NT,GO,KEGG,COG,and Swiss-Prot).Analyses of the ungerminated root primordium period,induced root primordium period,and root formation period detected 8,232,6,907,and 10,687 differentially expressed genes related to 133,132,and 133 metabolic pathways,respectively.Two significantly differentially expressed genes(Unigene13430_All and CL10096.Contig1_All)were associated with the auxin pathway.The full-length Unigene13430_All coding sequence(843 bp)encoded 280 amino acids,whereas the full-length CL10096.Contig1_All coding sequence(1,470 bp)encoded 489 amino acids.Unigene13430_All and CL10096.Contig1_All were identified as IAA gene family members and were respectively named PsIAA27 and PsARF19.The qRT-PCR analysis and functional verification indicated that the expressions of PsARF19 and PsIAA27 in tree peony seedlings,cuttings and grafted seedlings were significant different.PsARF19 promoted root development,it might be a regulatory gene related to the formation of tree peony roots,while PsIAA27 inhibited lateral root development,and it might be involved in controlling auxin sensitivity during root formation.The results of this study may form the basis of future investigations on the mechanism mediating peony root formation.The transcriptome data will be an excellent resource for researchers interested in characterizing the rooting-related tree peony genes.
基金supported by the Natural Science Foundation of Jiangsu Province of China(Grant number BK20180825)supported by the National Natural Science Foundation of China(31872732).
文摘Meyoid derived suppressor cells(MDSCs)are heterogeneous myeloid cell populations that expand in pathological conditions such as cancer,autoimmunity,and infection;these cells are also known to exert strong immunosuppressive effects on primitive Tcell responses.1 MDSCs are derived from myeloid progenitors and are arrested at an immature phase of differentiation.In mice,normal bone marrow contains~20–30%cells exhibiting the MDSC phenotype.2 In contrast,a very low number of MDSCs are found in naive spleens(2–4%),and these cells are completely absent in lymph nodes.