Determination of Kaempferol Content in 10 Batches of Different Production Areas of Yao Medicine Lysimachia foenum-graecum Hance

[Objectives]To determine the kaempferol content in Yao medicine Lysimachia foenum-graecum Hance.[Methods]The high performance liquid chromatography(HPLC)method was used.The chromatographic column:Inertsil ODS-3 C18 column(4.60 mm×250 mm,5μm);mobile phase:acetonitrile-0.1%glacial acetic acid;flow rate:1.0 mL/min;column temperature:30℃;detection wavelength:360 nm;sample injection volume:10μL.[Results]The kaempferol content in Yao medicine L.foenum-graecum was determined,Y=20652X-151098(R=0.9997)shows a good linear relationship within the linear range of 9.80-156.8μg/mL.[Conclusions]The high performance liquid chromatography(HPLC)method was established for determining the kaempferol content in Yao medicine L.foenum-graecum.This method is expected to provide a scientific basis for the formulation of quality control methods for L.foenum-graecum.

Engineering Yarrowia lipolytica to produce nutritional fatty acids:Current status and future perspectives

Due to their vital physiological functions,nutritional fatty acids have great potential as nutraceutical food supplements for preventing an array of diseases such as inflammation,depression,arthritis,osteoporosis,diabetes and cancer.Microbial biosynthesis of fatty acids follows the trend of sustainable development,as it enables green,environmentally friendly and efficient production.As a natural oleaginous yeast,Yarrowia lipolytica is especially well-suited for the production of fatty acids.Moreover,it has a variety of genetic engineering tools and novel metabolic engineering strategies that make it a robust workhorse for the production of an array of value-added products.In this review,we summarize recent advances in metabolic engineering strategies for accumulating nutritional fatty acids in Y.lipolytica,including conjugated fatty acids and polyunsaturated fatty acids.In addition,the future prospects of nutritional fatty acid production using the Y.lipolytica platform are discussed in light of the current progress,challenges,and trends in this field.Finally,guidelines for future studies are also emphasized.