The energy resources mainly petroleum and petroleum hydrocarbons are major pollutants of the environment.The oil and oil products contamination may cause severe harm and hence,the attention has been remunerated in the...The energy resources mainly petroleum and petroleum hydrocarbons are major pollutants of the environment.The oil and oil products contamination may cause severe harm and hence,the attention has been remunerated in the development of alternative technologies for elimination of these contaminants.Biosurfactants were used in the remediation of oil pollution due to advantages such as biodegradability and low toxicity.The biosurfactants are produced from low cost substrates like agro-industrial wastes which reduce the cost of production.Biosurfactants and bioemulsifiers are amphiphilic compounds and are produced as extracellular or a part of the cell membrane by bacteria.The insight view,how hydrocarbons are degraded by microorganisms and thereby reduce the damage of ecosystem is highly essential to target the problem.Biofilms are the bacterial communities which protects the bacterial cells from various adverse conditions.The present review describes the biosurfactants and its synthesis from bacteria and also emphases on the role of surfactants in oil remediation.展开更多
CRISPR genome editing utilizes Cas9 nuclease and single guide RNA(sgRNA),which directs the nuclease to a specific site in the genome and makes a double-stranded break(DSB).Design of sgRNA for CRISPR-Cas targeting,and ...CRISPR genome editing utilizes Cas9 nuclease and single guide RNA(sgRNA),which directs the nuclease to a specific site in the genome and makes a double-stranded break(DSB).Design of sgRNA for CRISPR-Cas targeting,and to promote CRISPR adaptation,uses a regulatory mechanism that ensures maximum CRISPR-Cas9 system functions when a bacterial population is at highest risk of phage infection.Acinetobacter baumannii is the most regularly identified gram-negative bacterium infecting patients.Recent reports have demonstrated that the extent of diseases caused by A.baumannii is expanding and,in a few cases,now surpasses the quantity of infections caused by P.aeruginosa.Most Acinetobacter strains possess biofilm-forming ability,which plays a major role in virulence and drug resistance.Biofilm bacteria use quorum sensing,a cell-to-cell communication process,to activate gene expression.Many genes are involved in biofilm formation and the mechanism to disrupt the biofilm network is still not clearly understood.In this study,we performed in silico gene editing to exploit the AbaI gene,responsible for biofilm formation.The study explored different tools available for genome editing to create gene knockouts,selecting the A.baumannii AbaI gene as a target.展开更多
基金The authors would like to acknowledge the support of VFSTR(Deemed to be University),Guntur,India.
文摘The energy resources mainly petroleum and petroleum hydrocarbons are major pollutants of the environment.The oil and oil products contamination may cause severe harm and hence,the attention has been remunerated in the development of alternative technologies for elimination of these contaminants.Biosurfactants were used in the remediation of oil pollution due to advantages such as biodegradability and low toxicity.The biosurfactants are produced from low cost substrates like agro-industrial wastes which reduce the cost of production.Biosurfactants and bioemulsifiers are amphiphilic compounds and are produced as extracellular or a part of the cell membrane by bacteria.The insight view,how hydrocarbons are degraded by microorganisms and thereby reduce the damage of ecosystem is highly essential to target the problem.Biofilms are the bacterial communities which protects the bacterial cells from various adverse conditions.The present review describes the biosurfactants and its synthesis from bacteria and also emphases on the role of surfactants in oil remediation.
文摘CRISPR genome editing utilizes Cas9 nuclease and single guide RNA(sgRNA),which directs the nuclease to a specific site in the genome and makes a double-stranded break(DSB).Design of sgRNA for CRISPR-Cas targeting,and to promote CRISPR adaptation,uses a regulatory mechanism that ensures maximum CRISPR-Cas9 system functions when a bacterial population is at highest risk of phage infection.Acinetobacter baumannii is the most regularly identified gram-negative bacterium infecting patients.Recent reports have demonstrated that the extent of diseases caused by A.baumannii is expanding and,in a few cases,now surpasses the quantity of infections caused by P.aeruginosa.Most Acinetobacter strains possess biofilm-forming ability,which plays a major role in virulence and drug resistance.Biofilm bacteria use quorum sensing,a cell-to-cell communication process,to activate gene expression.Many genes are involved in biofilm formation and the mechanism to disrupt the biofilm network is still not clearly understood.In this study,we performed in silico gene editing to exploit the AbaI gene,responsible for biofilm formation.The study explored different tools available for genome editing to create gene knockouts,selecting the A.baumannii AbaI gene as a target.
