Senescence is a cell fate that can be induced by intra/extracellular stresses such as DNA damage,oncogenic mutations,telomere shorting,and inflammation[1].Senescent cells develop a characteristic secretion profile inc...Senescence is a cell fate that can be induced by intra/extracellular stresses such as DNA damage,oncogenic mutations,telomere shorting,and inflammation[1].Senescent cells develop a characteristic secretion profile including a range of cytokines,chemokines,proteases,and other factors,which is termed the senescence-associated secretory phenotype(SASP)[2].Accumulating evidence indicates that SASP factors provoke multiple chronic diseases,even tumorigenesis and aggressiveness[3].Our recent studies reveal that heavy ion could induce senescence with lower dose than X-ray in various tumor cells[4;5].However,very little is known about the SASP of senescent tumor cells induced by heavy ions.In the present study,human melanoma cell line 92-1 cells were exposed to 10 Gy of carbon ion beams or X-rays,respectively.The cell culture supernatants were harvested 3 days post-irradiation,and the SASP were measured using human antibody arrays including 507 factors.展开更多
文摘Senescence is a cell fate that can be induced by intra/extracellular stresses such as DNA damage,oncogenic mutations,telomere shorting,and inflammation[1].Senescent cells develop a characteristic secretion profile including a range of cytokines,chemokines,proteases,and other factors,which is termed the senescence-associated secretory phenotype(SASP)[2].Accumulating evidence indicates that SASP factors provoke multiple chronic diseases,even tumorigenesis and aggressiveness[3].Our recent studies reveal that heavy ion could induce senescence with lower dose than X-ray in various tumor cells[4;5].However,very little is known about the SASP of senescent tumor cells induced by heavy ions.In the present study,human melanoma cell line 92-1 cells were exposed to 10 Gy of carbon ion beams or X-rays,respectively.The cell culture supernatants were harvested 3 days post-irradiation,and the SASP were measured using human antibody arrays including 507 factors.
