目的探讨加味补阳还五汤在视神经挫伤大鼠损伤修复中的具体作用机制,为临床提供实验依据。方法30只SD大鼠随机分为药物组、生理盐水组及空白对照组,前两组大鼠右眼建立视神经损伤模型后分别给予加味补阳还五汤、生理盐水灌胃,空白对照...目的探讨加味补阳还五汤在视神经挫伤大鼠损伤修复中的具体作用机制,为临床提供实验依据。方法30只SD大鼠随机分为药物组、生理盐水组及空白对照组,前两组大鼠右眼建立视神经损伤模型后分别给予加味补阳还五汤、生理盐水灌胃,空白对照组不做处理。分别于造模前及造模后1 d、1周、2周、4周、6周,各组均行闪光视觉诱发电位观察P波振幅和潜伏期的变化,HE染色观察视网膜组织形态学改变,免疫组织化学染色检测视网膜Caspase-3蛋白表达的变化。结果闪光视觉诱发电位结果显示,造模后4周、6周,药物组P波潜伏期明显缩短,P波振幅趋于平稳,与生理盐水组相比差异均有统计学意义(均为P<0.05)。HE染色结果显示,空白对照组视网膜组织结构层次清晰,药物组和生理盐水组造模后可见视网膜组织结构紊乱,明显水肿;随时间推移与生理盐水组相比,药物组的视网膜组织形态逐渐恢复,视网膜神经节细胞形态逐渐接近空白对照组。免疫组织化学染色结果显示,生理盐水组及药物组均在造模后1 d Caspase-3蛋白开始表达,造模后1周达到峰值,而后逐渐减少;与生理盐水组相比,造模后2周、4周、6周药物组Caspase-3蛋白表达均显著降低(均为P<0.05)。结论加味补阳还五汤在大鼠视神经损伤后能改善视神经的损伤,促进视神经修复。展开更多
The triple-shelled hollow spheres with optical properties were prepared via reversible addition-fragmentation chain transfer (RAFT) polymerization.After removal the core templates of the poly(styrene),the hollow silic...The triple-shelled hollow spheres with optical properties were prepared via reversible addition-fragmentation chain transfer (RAFT) polymerization.After removal the core templates of the poly(styrene),the hollow silica spheres were obtained.The coating process of poly(methyl methacrylate)(PMMA) on the hollow silica spheres surface via surface RAFT polymerization was performed subsequently.The polymers coated on the hollow spheres surfaces were end-functionalized by trithiocarbonate,and they were used as RAFT agent to proceed the chain extension polymerization using Tb complex as monomer.The samples were characterized by FT-IR,SEM and luminescence spectroscopy respectively.The results indicated that the triple-shelled hollow spheres had been prepared successfully and the average diameter of the hollow core was about 1μm.展开更多
文摘目的探讨加味补阳还五汤在视神经挫伤大鼠损伤修复中的具体作用机制,为临床提供实验依据。方法30只SD大鼠随机分为药物组、生理盐水组及空白对照组,前两组大鼠右眼建立视神经损伤模型后分别给予加味补阳还五汤、生理盐水灌胃,空白对照组不做处理。分别于造模前及造模后1 d、1周、2周、4周、6周,各组均行闪光视觉诱发电位观察P波振幅和潜伏期的变化,HE染色观察视网膜组织形态学改变,免疫组织化学染色检测视网膜Caspase-3蛋白表达的变化。结果闪光视觉诱发电位结果显示,造模后4周、6周,药物组P波潜伏期明显缩短,P波振幅趋于平稳,与生理盐水组相比差异均有统计学意义(均为P<0.05)。HE染色结果显示,空白对照组视网膜组织结构层次清晰,药物组和生理盐水组造模后可见视网膜组织结构紊乱,明显水肿;随时间推移与生理盐水组相比,药物组的视网膜组织形态逐渐恢复,视网膜神经节细胞形态逐渐接近空白对照组。免疫组织化学染色结果显示,生理盐水组及药物组均在造模后1 d Caspase-3蛋白开始表达,造模后1周达到峰值,而后逐渐减少;与生理盐水组相比,造模后2周、4周、6周药物组Caspase-3蛋白表达均显著降低(均为P<0.05)。结论加味补阳还五汤在大鼠视神经损伤后能改善视神经的损伤,促进视神经修复。
基金a project of Shandong Province Higher Education Science and Technology Program(No.J09LD56)the Scientific Research Start up Fund for Doctor of Liaocheng University.
文摘The triple-shelled hollow spheres with optical properties were prepared via reversible addition-fragmentation chain transfer (RAFT) polymerization.After removal the core templates of the poly(styrene),the hollow silica spheres were obtained.The coating process of poly(methyl methacrylate)(PMMA) on the hollow silica spheres surface via surface RAFT polymerization was performed subsequently.The polymers coated on the hollow spheres surfaces were end-functionalized by trithiocarbonate,and they were used as RAFT agent to proceed the chain extension polymerization using Tb complex as monomer.The samples were characterized by FT-IR,SEM and luminescence spectroscopy respectively.The results indicated that the triple-shelled hollow spheres had been prepared successfully and the average diameter of the hollow core was about 1μm.
