微合金化低碳马氏体钢的显微组织及力学性能

制备了钛、铌和钼等元素掺杂的微合金化低碳马氏体钢,并对其进行热轧+880℃淬火+不同温度(170,190,210,230,250℃)回火处理,研究了不同热处理态试验钢的显微组织、拉伸性能和冲击性能,以190℃回火态钢为例分析了强韧化机制。结果表明:热轧态试验钢由铁素体和贝氏体组成,淬火态试验钢由铁素体和马氏体组成,不同温度回火态试验钢均由回火马氏体和少量铁素体组成,回火态试验钢的铁素体面积分数在15%~20%,低于热轧态和淬火态;淬火态试验钢的抗拉强度和布氏硬度最高;随着回火温度升高,回火态试验钢的抗拉强度和布氏硬度下降,断后伸长率、屈服强度和冲击吸收功均先增大后减小;190℃回火态试验钢的断后伸长率和冲击吸收功最高,拉伸断口和冲击断口处纤维区中韧窝占比最高,抗拉强度、布氏硬度和屈服强度适中,综合力学性能最好;190℃回火态试验钢中析出的纳米级小尺寸(Ti,Nb,Mo,Cr)C相起到沉淀强化作用,纳米级大尺寸(Ti,Nb,Mo,Cr)C相起细晶强化作用,大角度晶界占比较高,提高了裂纹扩展功,因此试验钢强度和韧性提高。

IQGAP3 promotes the progression of glioma as an immune and prognostic marker

Background:IQGAP3 plays a crucial role in regulating cell proliferation,division,and cytoskeletal organization.Abnormal expression of IQGAP3 has been linked to various tumors,but its function in glioma is not well understood.Methods:Various methods,including genetic differential analysis,single-cell analysis,ROC curve analysis,Cox regression,Kaplan-Meier analysis,and enrichment analysis,were employed to analyze the expression patterns,diagnostic potential,prognostic implications,and biological processes involving IQGAP3 in normal and tumor tissues.The impact of IQGAP3 on immune infiltration and the immune microenvironment in gliomas was evaluated using immunofluorescence.Additionally,the cBioPortal database was used to analyze copy number variations and mutation sites of IQGAP3.Experimental validation was also performed to assess the effects of IQGAP3 on glioma cells and explore underlying mechanisms.Results:High IQGAP3 expression in gliomas is associated with an unfavorable prognosis,particularly in wild-type IDH and 1p/19q non-codeleted gliomas.Enrichment analysis revealed that IQGAP3 is involved in regulating the cell cycle,PI3K/AKT signaling,p53 signaling,and PLK1-related pathways.Furthermore,IQGAP3 expression may be closely related to the immunosuppressive microenvironment of glioblastoma.BRD-K88742110 and LY-303511 are potential drugs for targeting IQGAP3 in anti-glioma therapy.In vitro experiments showed that downregulation of IQGAP3 inhibits the proliferation and migration of glioma cells,with the PLK1/PI3K/AKT pathway potentially playing a crucial role in IQGAP3-mediated glioma progression.Conclusion:IQGAP3 shows promise as a valuable biomarker for diagnosis,prognosis,and immunotherapeutic strategies in gliomas.

A Single-Board Integrated Millimeter-Wave Asymmetric Full-Digital Beamforming Array for B5G/6G Applications

In this article,a single-board integrated millimeter-wave(mm-Wave)asymmetric full-digital beamforming(AFDBF)array is developed for beyond-fifth-generation(B5G)and sixth-generation(6G)communications.The proposed integrated array effectively addresses the challenge of arranging a large number of ports in a full-digital array by designing vertical connections in a three-dimensional space and successfully integrating full-digital transmitting(Tx)and receiving(Rx)arrays independently in a single board.Unlike the traditional symmetric array,the proposed asymmetric array is composed of an 8×8 Tx array arranged in a square shape and an 8+8 Rx array arranged in an L shape.The center-to-center distance between two adjacent elements is 0.54k0 for both the Tx and Rx arrays,where k0 is the free-space wavelength at 27 GHz.The proposed AFDBF array possesses a more compact structure and lower system hardware cost and power consumption compared with conventional brick-type full-digital arrays.In addition,the energy efficiency of the proposed AFDBF array outperforms that of a hybrid beamforming array.The measurement results indicate that the operating frequency band of the proposed array is 24.25–29.50 GHz.An eight-element linear array within the Tx array can achieve a scanning angle ranging from-47°to+47°in both the azimuth and the elevation planes,and the measured scanning range of each eight-element Rx array is–45°to+45°.The measured maximum effective isotropic radiated power(EIRP)of the eight-element Tx array is 43.2 dBm at 28.0 GHz(considering the saturation point).Furthermore,the measured error vector magnitude(EVM)is less than 3%when 64-quadrature amplitude modulation(QAM)waveforms are used.

CRISPR–Cas9-mediated promoter editing of FERONIA-Like receptor 13 increases plant growth and disease resistance in rice

Receptor kinases play a pivotal role in detecting environmental signals,and consequently,gene pleiotropy is frequently observed within this family.However,the trade-off in trait expression resulting from gene pleiotropy poses a constraint on the utilization of such genes in agricultural breeding.In this study,we identified the receptor kinase gene FERONIA-Like Receptor 13(FLR13)as a pleiotropic gene influencing plant height,tillering,grain yield,and disease resistance.Using promoter editing,we generated novel alleles(FLR13T5T6-1,FLR13T5T6-2)that confer resistance to rice blast and increase per-plant yield.The knockout of the T5T6 segment alleviates the inhibitory effects of two transcription factors,OsGBP1 and OsWRKY53,on FLR13 expression.In summary,our study presents a promising avenue for enhancing the pivotal attributes of receptor-like kinases through a promoter-editing strategy.

Synergistic extraction and separation of valuable elements from high-alumina fly ash with carbochlorination method

A novel process was proposed for synergistic extraction and separation of valuable elements from high-alumina fly ash.A thermodynamic analysis revealed that to achieve effective carbochlorination,it is crucial to conduct carbochlorination of the fly ash within the temperature range from 700 to 1000℃.The experimental results demonstrated that under the optimal conditions,the carbochlorination efficiency for Al,Si,Ca,Ti,and Mg exceeded 81.18%,67.62%,58.87%,82.15%,and 59.53%,respectively.The XRD patterns indicated that Al and Si in the mullite phase(Al_(6)Si_(2)O_(13))were chlorinated during the carbochlorination process,resulting in the formation of mullite mesophases(Al_(4.75)Si_(1.25)O_(9.63) and Al_(1.83)Si_(1.08)O_(4.85)).After the carbochlorination process,Al was accumulated as AlCl_(3) in the condenser,while SiCl_(4) and TiCl_(4) were enriched in the exhaust gas,and CaCl_(2),MgCl_(2),and unreacted oxides remained in the residue for further recycling.

Semi-supervised surface defect detection of wind turbine blades with YOLOv4

Timely inspection of defects on the surfaces of wind turbine blades can effectively prevent unpredictable accidents.To this end,this study proposes a semi-supervised object-detection network based on You Only Looking Once version 4(YOLOv4).A semi-supervised structure comprising a generative adversarial network(GAN)was designed to overcome the difficulty in obtaining sufficient samples and sample labeling.In a GAN,the generator is realized by an encoder-decoder network,where the backbone of the encoder is YOLOv4 and the decoder comprises inverse convolutional layers.Partial features from the generator are passed to the defect detection network.Deploying several unlabeled images can significantly improve the generalization and recognition capabilities of defect-detection models.The small-scale object detection capacity of the network can be improved by enhancing essential features in the feature map by adding the concurrent spatial and channel squeeze and excitation(scSE)attention module to the three parts of the YOLOv4 network.A balancing improvement was made to the loss function of YOLOv4 to overcome the imbalance problem of the defective species.The results for both the single-and multi-category defect datasets show that the improved model can make good use of the features of the unlabeled images.The accuracy of wind turbine blade defect detection also has a significant advantage over classical object detection algorithms,including faster R-CNN and DETR.

Chien-physics-informed neural networks for solving singularly perturbed boundary-layer problems

A physics-informed neural network(PINN)is a powerful tool for solving differential equations in solid and fluid mechanics.However,it suffers from singularly perturbed boundary-layer problems in which there exist sharp changes caused by a small perturbation parameter multiplying the highest-order derivatives.In this paper,we introduce Chien's composite expansion method into PINNs,and propose a novel architecture for the PINNs,namely,the Chien-PINN(C-PINN)method.This novel PINN method is validated by singularly perturbed differential equations,and successfully solves the wellknown thin plate bending problems.In particular,no cumbersome matching conditions are needed for the C-PINN method,compared with the previous studies based on matched asymptotic expansions.

On the Identification of N-rich Metal-poor Field Stars with Future Chinese Space Station Telescope

During the long term evolution of globular clusters(GCs), some member stars are lost to the field. The recently found nitrogen-rich(N-rich) metal-poor field stars are promising candidates of these GC escapees, since N enhancement is the fingerprint of chemically enhanced populations in GCs. In this work, we discuss the possibility of identifying N-rich metal-poor field stars with the upcoming Chinese Space Station Telescope(CSST). We focus on the main survey camera with NUV, u, g, r, i, z, y filters and slitless spectrograph with a resolution about 200.The combination of UV sensitive equipment and prominent N-related molecular lines in the UV band bodes well for the identification: the color–color diagram of(u-g) versus(g-r) is capable of separating N-rich field stars from normal halo stars, if metallicity can be estimated without using the information on u-band photometry.Besides, the synthetic spectra show that a signal-to-noise ratio of 10 is sufficient to identify N-rich field stars. In the near future, a large sample of N-rich field stars found by CSST, combined with state-of-the-art N-body simulations will be crucial to deciphering GC-Galaxy co-evolution.

Surface Deposition of Ni(OH)_(2) and Lattice Distortion Induce the Electrochromic Performance Decay of NiO Films in Alkaline Electrolyte

NiO,an anodic electrochromic material,has applications in energy-saving windows,intelligent displays,and military camouflage.However,its electrochromic mechanism and reasons for its performance degradation in alkaline aqueous electrolytes are complex and poorly understood,making it challenging to improve NiO thin films.We studied the phases and electrochemical characteristics of NiO films in different states(initial,colored,bleached and after 8000 cycles)and identified three main reasons for performance degradation.First,Ni(OH)_(2)is generated during electrochromic cycling and deposited on the NiO film surface,gradually yielding a NiO@Ni(OH)_(2)core-shell structure,isolating the internal NiO film from the electrolyte,and preventing ion transfer.Second,the core-shell structure causes the mode of electrical conduction to change from first-to second-order conduction,reducing the efficiency of ion transfer to the surface Ni(OH)_(2)layer.Third,Ni(OH)_(2)and NiOOH,which have similar crystal structures but different b-axis lattice parameters,are formed during electrochromic cycling,and large volume changes in the unit cell reduce the structural stability of the thin film.Finally,we clarified the mechanism of electrochromic performance degradation of NiO films in alkaline aqueous electrolytes and provide a route to activation of NiO films,which will promote the development of electrochromic technology.

New application of intestinal obstruction catheter in enterocutaneous fistula:A case report

BACKGROUND Enterocutaneous fistula(ECF)is an abnormal connection between the gastrointestinal tract and the skin.ECF can lead to massive body fluid loss,hypercatabolism,and malnutrition.Therefore,nutritional support plays a crucial role in managing ECFs and promoting the healing of fistulas.For nutritional support,enteral nutrition(EN)is the preferred method when gastrointestinal function is recovering.Currently,various EN approaches have been applied for different anatomical positions of the ECF.However,the effectiveness of administering EN support for treating lower ECFs still needs further exploration and improvement.CASE SUMMARY We present the case of a 46-year-old male who underwent gastrointestinal stromal tumour resection.Six days after the surgery,the patient presented with fever,fatigue,severe upper abdominal pain,and septic shock.Subsequently,lower ECFs were diagnosed through laboratory and imaging examinations.In addition to symptomatic treatment for homeostasis,total parenteral nutrition support was administered in the first 72 h due to dysfunction of the intestine.After that,we gradually provided EN support through the intestinal obstruction catheter in consideration of the specific anatomic position of the fistula instead of using the nasal jejunal tube.Ultimately,the patient could receive optimal EN support via the catheter,and no complications were found during the treatment.CONCLUSION Nutritional support is a crucial element in ECF management,and intestinal obstruction catheters could be used for early EN administration.

Effect of Rigid Pitch Motion on Flexible Vibration Characteristics of a Wind Turbine Blade

Adynamic pitch strategy is usually adopted to improve the aerodynamic performance of the blade of awind turbine.The dynamic pitch motion will affect the linear vibration characteristics of the blade.However,these influences have not been studied in previous research.In this paper,the influences of the rigid pitch motion on the linear vibration characteristics of a wind turbine blade are studied.The blade is described as a rotating cantilever beam with an inherent coupled rigid-flexible vibration,where the rigid pitch motion introduces a parametrically excited vibration to the beam.Partial differential equations governing the nonlinear coupled pitch-bend vibration are proposed using the generalized Hamiltonian principle.Natural vibration characteristics of the inherent coupled rigid-flexible system are analyzed based on the combination of the assumed modes method and the multi-scales method.Effects of static pitch angle,rotating speed,and characteristics of harmonic pitch motion on flexible natural frequencies andmode shapes are discussed.It shows that the pitch amplitude has a dramatic influence on the natural frequencies of the blade,while the effects of pitch frequency and pith phase on natural frequencies are little.

Temporal and spatial variations hydrochemical components and driving factors in Baiyangdian Lake in the Northern Plain of China

Understanding the temporal and spatial variation of hydrochemical components in large freshwater lakes is crucial for effective management and conversation.In this study,we identify the temporalspatial characteristics and driving factors of the hydrochemical components in Baiyangdian Lake using geochemical methods(Gibbs diagram,Piper diagram and End-element diagram of ion ratio)and multivariate statistical techniques(Principal component analysis and Correlation analysis).16 sets of samples were collected from Baiyangdian Lake in May(normal season),July(flood season),and December(dry season)of 2022.Results indicate significant spatial variation in Nat,ci,SO and NO,,suggesting a strong influence of human activities.Cation concentrations exhibit greater seasonal variation in the dry season compared to the flood season,while the concentrations of the four anions show inconsistent seasonal changes due to the combined effects of river water chemical composition and human activities.The hydrochemical type of Baiyangdian Lake is primarily HCO,Cl-Na.Ca,Mg*and HCO,originate mainly from silicate and carbonate rock dissolution,while Kt,Nat and CI originate mainly from sewage and salt dissolution in sediments.SO42 may mainly stem from industrial wastewater,while NO,primarily originates from animal feces and domestic sewage.Through the use of Principal Component Analysis,it is identified that water-rock interaction(silicate and carbonate rocks dissolution,and dissolution of salt in sediments),carbonate sedimentation,sewage,agricultural fertilizer and manure,and nitrification are the main driving factors of the variation of hydrochemical components of Baiyangdian Lake across three hydrological seasons.These findings suggest the need for effective control of substandard domestic sewage discharge,optimization of agricultural fertilization strategies,and proper management of animal manure to comprehensively improve the water environment in Baiyangdian Lake.

个体化诊疗模式在胃肠道恶性肿瘤中预防中度致吐性化疗方案的临床观察

目的:分析真实世界中化疗引起的恶心和呕吐(chemotherapy-induced nausea and vomiting,CINV)的个体化诊疗模式对胃肠道恶性肿瘤患者的CINV控制效果及对生存质量的影响。方法:选取2018年10月至2020年10月就诊于河北医科大学第四医院接受中度致吐风险化疗方案的289例胃肠道恶性肿瘤患者,按照CINV评估治疗方法分为CINV个体化诊疗组(A组)和医师选择诊疗组(B组),分析比较两组CINV控制效果及对生存质量的影响。结果:289例患者中A组146例,B组143例。A组全程恶心(74.66%vs.34.27%,P<0.001)、呕吐(87.67%vs.55.94%,P<0.001)完全控制率均明显优于B组,A组急性期及延迟期恶心、呕吐完全控制率优于B组。同时,A组患者在生存质量的功能领域(躯体功能、角色功能、社会功能),症状领域(恶心呕吐、疲倦、失眠、厌食),以及总体健康状况方面均表现出显著的优势。结论:个体化CINV诊疗模式可显著改善真实世界中胃肠道恶性肿瘤患者的CINV控制率和生存质量。

胃癌骨转移患者临床病理特征及预后影响因素分析

目的对胃癌骨转移患者临床病理特征及预后进行回顾性分析。方法收集2005~2015年收治的胃癌骨转移患者139例,分析患者临床病理特征和治疗对患者生存时间的影响。结果胃癌患者常见的骨转移部位依次为脊柱、骨盆及肋骨。胃癌骨转移患者中位生存时间15.1个月,确诊骨转移后患者中位生存时间为5.7个月。TNM分期、同时或异时性骨转移、是否化疗及是否双膦酸盐治疗是影响胃癌骨转移患者总生存期的独立预后因素(P<0.05)。而骨转移后是否接受化疗是影响患者骨转移后生存期的独立预后因素(P<0.05)。结论骨转移多发生于胃癌病程晚期,患者一旦发生骨转移则提示预后差,化疗可延长患者骨转移后的生存时间。临床医生应注意筛查并采取积极的治疗措施延长患者生存期。

Research on the Strategy of Integrating Red Culture into Patriotic Education Activities of Young People

Young people are the flowers of the motherland, is the future of the motherland, youth teachers have great responsibilities. Integrate red culture into daily teaching activities, inherit and carry forward, enhance children’s values, cultivate children’s excellent qualities such as patriotism and selfless dedication, improve children’s comprehensive quality, and let children grow up healthily. Taking red culture as the starting point, this paper discusses the strategy of integrating red cultural resources into patriotic education activities of teenagers, points out the existing problems in the current integration of red culture into patriotic education activities of teenagers and finds out the reasons;clarify the relevant concepts of red culture and patriotic education activities for young people;from the three aspects of integrating curriculum resources to enhance the significance of red culture education, creating a red environment to create a red education atmosphere, and digging deep into red cultural resources, this paper discusses the strategy of integrating red culture into patriotic education activities for young people, aiming to promote the organic combination of patriotic education activities and red culture for young people, and increase national and cultural self-confidence.

超声空化作用下高温合金废料中战略金属的浸出机理

为提高高温合金废料中战略金属的回收效率,提出一种超声辅助浸出工艺。在超声辅助浸出过程中,浸出时间为60 min时,高温合金废料中Re、Ni、Co、Al、Cr和W的浸出率分别为92.3%、95.2%、98.5%、98.7%、97.5%和27.2%,明显优于常规浸出工艺中浸出时间为120 min时的浸出效率。高温合金废料中战略金属浸出效率的提高可归因于超声空化作用下产生的物理和化学协同作用。超声空化的物理作用可以阻止高温合金废料表面溶解层的形成,促进浸出液与高温合金废料接触,从而提高高温合金废料中战略金属的浸出率;而超声空化的化学作用可以促进HCl-H_(2)O_(2)体系中羟基自由基的产生,从而增强浸出体系的电位,促进体系中Re向ReO_(4)^(-)的氧化反应过程。

Effects of neuregulin-1 on autonomic nervous system remodeling post-myocardial infarction in a rat model

Sympathetic nerve and vagus nerve remodeling play an important part in cardiac function post-myocardial infarction （MI）. Increasing evidence indicates that neuregulin-1 （NRG-1） improves cardiac function following heart failure. Since its impact on cardiac function and neural remodeling post-MI is poorly understood, we aimed to investigate the role of NRG-1 in autonomic nervous system remodeling post-MI. Forty-five Sprague-Dawley rats were equally randomized into three groups： sham （with the left anterior descending coronary artery exposed but without ligation）, MI （left anterior descending coronary artery ligation）, and MI plus NRG-1 （left anterior descending coronary artery ligation followed by intraperitoneal injection of NRG-1 （10 lag/kg, once daily for 7 days））. At 4 weeks after MI, echocardi- ography was used to detect the rat cardiac function by measuring the left ventricular end-systolic inner diameter, left ventricular diastolic diameter, left ventricular end-systolic volume, left ventricular end-diastolic volume, left ventricular ejection fraction, and left ventricular fractional shortening, mRNA and protein expression levels of tyrosine hydroxylase, growth associated protein-43 （neuronal specific pro- tein）, nerve growth factor, choline acetyltransferase （vagus nerve marker）, and vesicular acetylcholine transporter （cardiac vagal nerve fiber marker） in ischemic myocardia were detected by real-time PCR and western blot assay to assess autonomous nervous remodeling. After MI, the rat cardiac function deteriorated significantly, and it was significantly improved after NRG-1 injection. Compared with the MI group, mRNA and protein levels of tyrosine hydroxylase and growth associated protein-43, as well as choline acetyltransferase mRNA level significantly decreased in the MI plus NRG-1 group, while mRNA and protein levels of nerve growth factor and vesicular acetylcholine transporters, as well as choline acetyltransferase protein level slightly decreased. Our results indicate that NRG- 1 can improve cardiac function and regulate sympathetic and vagus nerve remodeling post-MI, thus reaching a new balance of the autonomic nervous system to protect the heart from injury.

A novel noise reduction technique for underwater acoustic signals based on complete ensemble empirical mode decomposition with adaptive noise,minimum mean square variance criterion and least mean square adaptive filter

Underwater acoustic signal processing is one of the research hotspots in underwater acoustics.Noise reduction of underwater acoustic signals is the key to underwater acoustic signal processing.Owing to the complexity of marine environment and the particularity of underwater acoustic channel,noise reduction of underwater acoustic signals has always been a difficult challenge in the field of underwater acoustic signal processing.In order to solve the dilemma,we proposed a novel noise reduction technique for underwater acoustic signals based on complete ensemble empirical mode decomposition with adaptive noise(CEEMDAN),minimum mean square variance criterion(MMSVC) and least mean square adaptive filter(LMSAF).This noise reduction technique,named CEEMDAN-MMSVC-LMSAF,has three main advantages:(i) as an improved algorithm of empirical mode decomposition(EMD) and ensemble EMD(EEMD),CEEMDAN can better suppress mode mixing,and can avoid selecting the number of decomposition in variational mode decomposition(VMD);(ii) MMSVC can identify noisy intrinsic mode function(IMF),and can avoid selecting thresholds of different permutation entropies;(iii) for noise reduction of noisy IMFs,LMSAF overcomes the selection of deco mposition number and basis function for wavelet noise reduction.Firstly,CEEMDAN decomposes the original signal into IMFs,which can be divided into noisy IMFs and real IMFs.Then,MMSVC and LMSAF are used to detect identify noisy IMFs and remove noise components from noisy IMFs.Finally,both denoised noisy IMFs and real IMFs are reconstructed and the final denoised signal is obtained.Compared with other noise reduction techniques,the validity of CEEMDAN-MMSVC-LMSAF can be proved by the analysis of simulation signals and real underwater acoustic signals,which has the better noise reduction effect and has practical application value.CEEMDAN-MMSVC-LMSAF also provides a reliable basis for the detection,feature extraction,classification and recognition of underwater acoustic signals.

Rig-I^-/- mice develop colitis associated with downregulation of Gαi2

RIG-I （retinoid acid-inducible gene-I）, a putative RNA helicase with a cytoplasmic caspase-recrultment domain （CARD）, was identified as a pattem-recognition receptor （PRR） that mediates antiviral immunity by inducing type I interferon production. To further study the biological function of RIG-I, we generated Rig-I^-/- mice through homologous recombination, taking a different strategy to the previously reported strategy. Our Rig-I^-/- mice are viable and fertile. Histological analysis shows that Rig-I^-/ mice develop a colitis-like phenotype and increased susceptibility to dextran sulfate sodium-induced colitis. Accordingly, the size and number of Peyer＇s patches dramatically decreased in mutant mice. The peripheral T-cell subsets in mutant mice are characterized by an increase in effector T cells and a decrease in naive T cells, indicating an important role for Rig-I in the regulation ofT-cell activation. It was further found that Rig-I deficiency leads to the downregulation of G protein αi2 subunit （Gαi2） in various tissues, including T and B lymphocytes. By contrast, upregulation of Rig-I in NB4 cells that are treated with ATRA is accompanied by elevated Gαi2 expression. Moreover, Gαi2 promoter activity is increased in co-transfected NIH3T3 cells in a Rig-I dose-dependent manner. All these findings suggest that Rig-I has crucial roles in the regulation of Gαi2 expression and T-cell activation. The development of colitis may be, at least in part, associated with downregulation of Gαi2 and disturbed T-cell homeostasis.

Comparison of gene expression profiles between primary tumor and metastatic lesions in gastric cancer patients using laser microdissection and cDNA microarray

AIM. To study the differential gene expression profiles of target cells in primary gastric cancer and its metastatic lymph nodes using laser microdissection （LMD） in combination with cDNA microarray. METHODS： Normal gastric tissue samples from 30 healthy individuals, 36 cancer tissue samples from primary gastric carcinoma and lymph node metastasis tissue samples from 58 patients during gastric cancer resection were obtained using LMD in combination with cDNA microarray independently. After P27-based amplification, aRNA from 36 of 58 patients （group 1） with lymph node metastasis and metastatic tissue specimens from the remaining 22 patients （group 2） were applied to cDNA microarray. Semiquantitative reverse transcriptase polymerase chain reaction （RT-PCR） and imrnunohistochemical assay verified the results of microarray in group 2 and further identified genes differentially expressed in the progression of gastric cancer. RESULTS： The expression of 10 genes was up-regulated while the expression of 15 genes was down-regulated in 22 gastric carcinoma samples compared with that of genes in the normal controls. The results were confirmed at the level of mRNA and protein, and suggested that four genes （OPCML, RNASE1, YES1 and ACK1） could play a key role in the tumorigenesis and metastasis of gastric cancer. The expression pattern of 3 genes （OPCML, RNASE1 and YES1） was similar to tumor suppressor genes. For example, the expression level of these genes was the highest in normal gastric epithelium, which was decreased in primary carcinoma, and further decreased in metastatic lymph nodes. On the contrary, the expression pattern of gene ACK1 was similar to that of oncogene. Four genes were further identified as differentially expressed genes in the majority of the cases in the progression of gastric cancer. CONCLUSION： LMD in combination with cDNA microaro ray provides a unique support foe the identification of early expression profiles of differential genes and the expression pattern of 3 genes （OPCML, RNASE1 and YES1） associated with the progression of gastric cancer. Further study is needed to reveal the molecular mechanism of lymph node metastasis in patients with gastric cancer.