The first distinct mark of rodent implantation is the increased vascular permeability and significant angio-genesis at the sites of blastocyst implantation, but its mechanism is not clearly defined. Vascular endotheli...The first distinct mark of rodent implantation is the increased vascular permeability and significant angio-genesis at the sites of blastocyst implantation, but its mechanism is not clearly defined. Vascular endothelial growth factor (VEGF) is the key mediator for angiogenesis during em-bryogenesis and adult span and also serves as a vascular permeability factor. The aim of this study is to explore VEGF regulation mechanism and the possible role that VEGF plays in implantation by studying the VEGF expression and angiogenesis in the rat uterus during estrous cycle, ovarioec-tomized and peri-implantation stages using in situ message RNA hybridization and confocal laser scanning techniques. The results indicated that VEGF was regulated by ovarian steroid hormones. VEGF expression before implantation was localized at luminal epithelium, shifted to stroma as implantation initiated and extensively located at the decidualizing stroma region after implantation. Bandeiraea simplicifolia-1 (BS-1) agglutinin and展开更多
Neovascularization, i.e. new blood vessels formation, can be divided into two different processes: vasculogene-sis, whereby a primitive vascular network is established during embryogenesis from multipotential mesenchy...Neovascularization, i.e. new blood vessels formation, can be divided into two different processes: vasculogene-sis, whereby a primitive vascular network is established during embryogenesis from multipotential mesenchymal progenitors; and angiogenesis, which refers to the new blood vessels formation from pre-existing vessels[1,2].Angiogenesis contributes to the most process throughout the whole life span from embryonic development to adult growth’21. In this meaning, neovascularization is usually used to imply angiogenesis. Under physiological conditions, angiogenesis is a strictly regulated event and rarely happens in most adult tissues except for fracture or healing of wounds[2,3]. However, a notable phenomenon is that the tissues of ovary and uterine endometnum are unique in the cycle-specific changes in vascularity that occur in each estrous/menstrual cycle. Active angiogenesis occurs in placenta to satisfy the needs of embryonic implantation and development. Defects in angiogenesis are associated展开更多
基金This work was supported by Special Funds for the Major State Basic Research Project (Grant No. G1999055903)and the Funds of Innovation Engineering Project of the Chinese Academy of Sciences.
文摘The first distinct mark of rodent implantation is the increased vascular permeability and significant angio-genesis at the sites of blastocyst implantation, but its mechanism is not clearly defined. Vascular endothelial growth factor (VEGF) is the key mediator for angiogenesis during em-bryogenesis and adult span and also serves as a vascular permeability factor. The aim of this study is to explore VEGF regulation mechanism and the possible role that VEGF plays in implantation by studying the VEGF expression and angiogenesis in the rat uterus during estrous cycle, ovarioec-tomized and peri-implantation stages using in situ message RNA hybridization and confocal laser scanning techniques. The results indicated that VEGF was regulated by ovarian steroid hormones. VEGF expression before implantation was localized at luminal epithelium, shifted to stroma as implantation initiated and extensively located at the decidualizing stroma region after implantation. Bandeiraea simplicifolia-1 (BS-1) agglutinin and
基金This work was supported by the State Major Basic Research Project (Grant No. 1999055903)and the Knowledge Innovation Project of the Chinese Academy of Sciences.
文摘Neovascularization, i.e. new blood vessels formation, can be divided into two different processes: vasculogene-sis, whereby a primitive vascular network is established during embryogenesis from multipotential mesenchymal progenitors; and angiogenesis, which refers to the new blood vessels formation from pre-existing vessels[1,2].Angiogenesis contributes to the most process throughout the whole life span from embryonic development to adult growth’21. In this meaning, neovascularization is usually used to imply angiogenesis. Under physiological conditions, angiogenesis is a strictly regulated event and rarely happens in most adult tissues except for fracture or healing of wounds[2,3]. However, a notable phenomenon is that the tissues of ovary and uterine endometnum are unique in the cycle-specific changes in vascularity that occur in each estrous/menstrual cycle. Active angiogenesis occurs in placenta to satisfy the needs of embryonic implantation and development. Defects in angiogenesis are associated
