The N6-methylation of RNA adenosines(N6-methyladenosine,m^(6)A)is an important regulator of gene expression with critical implications in vertebrate and insect development.However,the developmental significance of epi...The N6-methylation of RNA adenosines(N6-methyladenosine,m^(6)A)is an important regulator of gene expression with critical implications in vertebrate and insect development.However,the developmental significance of epitranscriptomes in lophotrochozoan organisms remains unknown.Using methylated RNA immunoprecipitation sequencing(MeRIP-seq),we generated transcriptome-wide m^(6)A-RNA methylomes covering the entire development of the oyster from oocytes to juveniles.Oyster RNA classes display specific m^(6)A signatures,with messenger RNAs(mRNAs)and long non-coding RNAs(lncRNAs)exhibiting distinct profiles and being highly methylated compared to transposable element(TE)transcripts.Epitranscriptomes are dynamic and correspond to the chronological steps of development(cleavage,gastrulation,organogenesis,and metamorphosis),with minimal mRNA and lncRNA methylation at the morula stage followed by a global increase.mRNA m^(6)A levels are correlated with transcript levels,and shifts in methylation profiles correspond to expression kinetics.Differentially methylated transcripts cluster according to embryo-larval stages and bear the corresponding developmental functions(cell division,signal transduction,morphogenesis,and cell differentiation).The m^(6)A level of TE transcripts is also regulated and peaks during the gastrulation.We demonstrate that m^(6)A-RNA methylomes are dynamic and associated with gene expression regulation during oyster development.The putative epitranscriptome implication in the cleavage,maternal-to-zygotic transition,and cell differentiation in a lophotrochozoan model brings new insights into the control and evolution of developmental processes.展开更多
基金supported by the French national program CNRS EC2CO ECODYN(Ecosphère Continentale et Cótière’HERITAGe’Grant No.2019-200919362L)awarded to Guillaume Rivière and the Council of the Normandy Region(RIN ECUMEGrant No.18E01643-18P02383)awarded to Pascal Favrel.
文摘The N6-methylation of RNA adenosines(N6-methyladenosine,m^(6)A)is an important regulator of gene expression with critical implications in vertebrate and insect development.However,the developmental significance of epitranscriptomes in lophotrochozoan organisms remains unknown.Using methylated RNA immunoprecipitation sequencing(MeRIP-seq),we generated transcriptome-wide m^(6)A-RNA methylomes covering the entire development of the oyster from oocytes to juveniles.Oyster RNA classes display specific m^(6)A signatures,with messenger RNAs(mRNAs)and long non-coding RNAs(lncRNAs)exhibiting distinct profiles and being highly methylated compared to transposable element(TE)transcripts.Epitranscriptomes are dynamic and correspond to the chronological steps of development(cleavage,gastrulation,organogenesis,and metamorphosis),with minimal mRNA and lncRNA methylation at the morula stage followed by a global increase.mRNA m^(6)A levels are correlated with transcript levels,and shifts in methylation profiles correspond to expression kinetics.Differentially methylated transcripts cluster according to embryo-larval stages and bear the corresponding developmental functions(cell division,signal transduction,morphogenesis,and cell differentiation).The m^(6)A level of TE transcripts is also regulated and peaks during the gastrulation.We demonstrate that m^(6)A-RNA methylomes are dynamic and associated with gene expression regulation during oyster development.The putative epitranscriptome implication in the cleavage,maternal-to-zygotic transition,and cell differentiation in a lophotrochozoan model brings new insights into the control and evolution of developmental processes.
