Effects of Losartan on Cell Apoptosis and Expression of Caspase-3 and JNK Proteins in Kidney Tissue in Renal Interstitial Fibrosis Rats

[Objectives]To investigate the effects of losartan on cell apoptosis and the expression of caspase-3 and JNK proteins in kidney tissue in the adenine-induced renal fibrosis rats.[Methods]Thirty Wistar rats were randomly divided into three groups:control group(n=10),model group(n=10)and losartan group(n=10).The rats in the control group received saline,while those in the model group and losartan group both received adenine by gavage,for 21 d.After the renal interstitial fibrosis model was established,the rats in the losartan group were treated with losartan[10 mg/(kg·d)],while the rats in the control group and the model group rats were administered with the same amount of saline.The course of treatment was 30 d.Finally,the renal function,blood urea nitrogen(BUN),serum creatinine(Scr),creatinine clearance rate(Ccr)and the pathological morphology of the rats were detected.The apoptosis of renal tubular epithelial cells was tested by TUNEL.The caspase-3 and JNK protein expression was tested by Western blotting.[Results]After administering adenine for 21 d,the BUN,24 MTP and kidney/body weight in the model group were increased,significantly higher than the control group(P<0.01),and the Ccr was remarkably decreased(P<0.01),signifying that the renal interstitial fibrosis model was successfully built.After treating with losartan for 30 d,the Scr,BUN,and 24 MTP were significantly decreased(P<0.01),and the Ccr was significantly increased in the losartan group(P<0.01).In addition,in comparison to the model group,renal tubular epithelial apoptosis was decreased and caspase-3 and JNK expression was downregulated in the losartan group(P<0.05).[Conclusions]Losartan can reduce the adenine-induced elevation of Scr,BUN and 24 hMPT,increase Ccr,improve general condition of renal interstitial fibrosis in rats and ameliorate the progression of chronic kidney failure(CKD).The effectiveness of losartan is probably due to its ability to regulate caspase-3,JNK protein expression and attenuate renal cell apoptosis.

反相高效液相色谱法测定尿液中己内酰胺

目的建立尿中己内酰胺的反相高效液相色谱测定方法。方法尿液经乙腈沉淀,稀释定容后以5000 r/min高速离心,取上清液过0.45μm微孔滤膜后上机测定,采用Sunfire C18色谱柱分离,以乙腈-水(10:90,V/V)为流动相,在210 nm波长下紫外检测器检测。结果己内酰胺标准浓度为0 mg/L~200 mg/L时,线性关系良好(r=0.9999),方法定量限为0.42 mg/L,加标回收率为95.4%~106.1%,相对标准偏差(RSD)为1.8%~4.6%。在优化色谱条件下,多种酰胺类化合物均不干扰己内酰胺的测定。结论本方法简便、快速,灵敏度、准确度高,适于尿液中己内酰胺的测定。

Characteristics and sources of polycyclic aromatic hydrocarbons in impervious surface run-off in an urban area in Shanghai, China

Rainwater and run-off from three kinds of impervious surface in the Shanghai urban area,China were sampled.Polycyclic aromatic hydrocarbons(PAHs) were measured in the samples,and their sources were assessed.The mean sum of the 16 PAH concentrations measured in rainwater and run-offs from ceramic tiles,asphalt roofs,and asphalt roads were 873,1404,1743,and 4023 ng/L,respectively.The PAH concentrations found in this study were moderate compared to PAH concentrations found in run-offs in other studies.The main PAH components in the rainwater,roof run-off,and asphalt road run-off samples were 3-ring PAHs,3–4-ring PAHs,and 4–6-ring PAHs,respectively.Source apportionment results indicated that combustion(47.4%–55.5%) and vehicular emissions(30.5%–33.0%) were the major contributors to PAHs in roof run-off.Vehicular emissions were the most significant contributors to asphalt road run-off(47.2%),followed by combustion(23.5%),and petroleum(16.3%).Vehicular emissions and coal and natural gas combustion are therefore the most significant sources of PAHs in run-off from impervious surfaces in the Shanghai urban area.