Inflammation is closely related to stroke prognosis, and high inflammation status leads to poor functional outcome in stroke. DNA methylation is involved in the pathogenesis and prognosis of stroke. However, the effec...Inflammation is closely related to stroke prognosis, and high inflammation status leads to poor functional outcome in stroke. DNA methylation is involved in the pathogenesis and prognosis of stroke. However, the effect of DNA methylation on stroke at high levels of inflammation is unclear. In this study, we constructed a hyperinflammatory cerebral ischemia mouse model and investigated the effect of hypomethylation and hypermethylation on the functional outcome. We constructed a mouse model of transient middle cerebral artery occlusion and treated the mice with lipopolysaccharide to induce a hyperinflammatory state. To investigate the effect of DNA methylation on stroke, we used small molecule inhibitors to restrain the function of key DNA methylation and demethylation enzymes. 2,3,5-Triphenyltetrazolium chloride staining, neurological function scores, neurobehavioral tests, enzyme-linked immunosorbent assay, quantitative reverse transcription PCR and western blot assay were used to evaluate the effects after stroke in mice. We assessed changes in the global methylation status by measuring DNA 5-mc and DNA 5-hmc levels in peripheral blood after the use of the inhibitor. In the group treated with the DNA methylation inhibitor, brain tissue 2,3,5-triphenyltetrazolium chloride staining showed an increase in infarct volume, which was accompanied by a decrease in neurological scores and worsening of neurobehavioral performance. The levels of inflammatory factors interleukin 6 and interleukin-1 beta in ischemic brain tissue and plasma were elevated, indicating increased inflammation. Related inflammatory pathway exploration showed significant overactivation of nuclear factor kappa B. These results suggested that inhibiting DNA methylation led to poor functional outcome in mice with high inflammation following stroke. Further, the effects were reversed by inhibition of DNA demethylation. Our findings suggest that DNA methylation regulates the inflammatory response in stroke and has an important role in the functional outcome of hyperinflammatory stroke.展开更多
Background:Recently,ultra-processed foods(UPFs)have attracted considerable attention,leading to numerous studies worldwide.Scientometrics is currently gaining popularity among scientific communities,offering advantage...Background:Recently,ultra-processed foods(UPFs)have attracted considerable attention,leading to numerous studies worldwide.Scientometrics is currently gaining popularity among scientific communities,offering advantage of providing critical references to scholars of specific fields.Methods:This scientometric study aimed to analyze trends and hotspots of UPF research using English articles or reviews related to UPFs retrieved from the Web of Science Core Collection on March 5,2023.Two independent researchers selected the identified records on titles,abstracts,and author’s keywords,and the data were analyzed using R-bibliometrix,CiteSpace,and VOSviewer.Results:A total of 1018 publications(901 articles and 117 reviews)published from 2010 to 2023 were included.The result showed a significant increase in UPF publications over the past decades.Brazil published the highest number of papers(n=473),with over half of the top 10 active institutions were located in Brazil.The University of São Paulo contributed the most publications(n=206)and the 10 most productive authors belonged to this institution,with Monteiro CA and Levy RB contributing the most publications.The main research foundations included the NOVA system,the definition of UPFs,the prevalence of UPFs,and the impact of UPFs on dietary quality and health status.The main research frontiers included topics such as“systematic review,”“NOVA food classification,”“COVID-19,”“diabetes,”“pregnancy,”“food addiction,”“warning labels,”“plant-based diet,”and“commercial determinants of health”.Conclusions:This study provided a comprehensive overview of development trends and research hotspots of global UPF studies.展开更多
Objective: To summarize and evaluate the evidence of guidelines and systematic reviews(SRs) of nonpharmacological interventions for mild cognitive impairment(MCI) to support the development of future guidelines and cl...Objective: To summarize and evaluate the evidence of guidelines and systematic reviews(SRs) of nonpharmacological interventions for mild cognitive impairment(MCI) to support the development of future guidelines and clinical decisions for MCI patients.Methods: Scottish Intercollegiate Guideline Network(SIGN), National Institute for Health and Clinical Excellence(NICE), American Academy of Neurology(AAN), Registered Nurses Association of Ontario(RNAO), Web of Science, PubMed, Cochrane Library, CNAHL, VIP, China National Knowledge Infrastructure(CNKI), and Wanfang Database were searched for relevant publications, including guidelines and SRs, from January 2014 to March 2019. Two authors independently screened articles, extracted data, and assessed the publications for adherence to the inclusion criteria. Appraisal of Guidelines for Research and Evaluation(AGREE II) was used to assess the quality of the guidelines, and Assessment of Multiple Systematic Reviews(AMSTAR 2) was used to assess the quality of SRs. In addition, the Grading of Recommendations Assessment, Development and Evaluation(GRADE) approach was used to evaluate the quality of outcomes.Results: Thirty-two articles were retrieved, including 1 guideline and 31 SRs. Fourteen SRs of physical exercise for MCI, six articles describing cognitive interventions, four articles describing acupuncture, and seven articles assessing dietary interventions(including four articles employing a Mediterranean diet, one article using vitamin B supplementation, and two articles assessing the effects of tea, coffee, and caffeine) were included. The quality of the articles was very low for 4(13%), low for 10(32%), and moderate for 17(55%).Conclusions: Based on the evidence available to date, nonpharmacological interventions may improve the current cognitive function of persons with MCI. In particular, physical exercise, cognitive interventions, and acupuncture exerted promising effects. However, due to the limited number and quality of the included publications, additional high-quality reviews are needed to further confirm.展开更多
[目的]获取与轻度认知功能障碍(MCI)运动干预相关的指南、系统评价或Meta分析,对其进行证据总结与评价,以期为MCI病人相关指南推荐意见的制定提供参考性建议。[方法]计算机检索加拿大安大略注册护士协会(Registered Nurses Association ...[目的]获取与轻度认知功能障碍(MCI)运动干预相关的指南、系统评价或Meta分析,对其进行证据总结与评价,以期为MCI病人相关指南推荐意见的制定提供参考性建议。[方法]计算机检索加拿大安大略注册护士协会(Registered Nurses Association of Ontario,RNAO)、美国神经病协会(American Academy of Neurology,AAN)、英国国家卫生与临床优化研究所(National Institute for Health and Clinical Excellence,NICE)、苏格兰院际指南网(Scottish Intercollegiate Guideline Network,SIGN)、PubMed、Cochrane library、Web of science、CINAHL、CNKI、万方及维普数据库获得相关指南及系统评价,检索时限从2014年1月—2018年2月。采用AMSTAR 2评价工具对文献质量进行评价,必要时应用GRADE工具对证据体进行证据分级,针对指南的质量评价采用AGREEⅡ标准进行评价。[结果]最终纳入1篇指南,12篇系统评价/Meta分析,AMASTA 2评价结果显示,9篇文献为中等质量。GRADE证据质量评价显示,3个证据体证据质量为高质量,11个证据体为中等质量,10个证据体为低质量,3个证据体为极低质量。[结论]运动干预具有较好的疗效,可作为MCI病人的临床辅助治疗措施,但运动干预对MCI病人干预效果系统评价的方法学质量总体不高,其真实效果有待大样本、高质量的研究进一步探讨。展开更多
[目的]总结与评价非药物干预治疗轻度认知功能障碍(mild cognitive impairment,MCI)的指南及系统评价,为改善中老年人认知功能、延缓向老年性痴呆的转化提供依据。[方法]计算机检索加拿大安大略注册护士协会(Registered Nurses Associat...[目的]总结与评价非药物干预治疗轻度认知功能障碍(mild cognitive impairment,MCI)的指南及系统评价,为改善中老年人认知功能、延缓向老年性痴呆的转化提供依据。[方法]计算机检索加拿大安大略注册护士协会(Registered Nurses Association of Ontario,RNAO)、美国神经病协会(American Academy of Neurology,AAN)、英国国家卫生与临床优化研究所(National Institute for Health and Clinical Excellence,NICE)、苏格兰院际指南网(Scottish Intercollegiate Guideline Network,SIGN)、Cochrane Library、PubMed、Web of Science、CINAHL、中国知网(CNKI)、万方及维普数据库获得相关指南及系统评价。[结果]共纳入30篇文献,1篇指南,重点介绍了对MCI病人进行评估和筛查、MCI病人药物及非药物治疗;29篇系统评价,包括4篇针刺疗法治疗MCI系统评价,7篇饮食干预治疗MCI系统评价(包括4篇地中海饮食,1篇补充维生素B,3篇咖啡摄入),12篇运动干预治疗MCI系统评价(包括太极运动、有氧运动等),4篇认知干预治疗MCI系统评价,2篇质性研究系统评价。[结论]非药物干预治疗MCI研究有重要意义,且大量研究显示有较好疗效,可作为MCI病人的临床辅助治疗措施。展开更多
基金supported by the National Natural Science Foundation of China,No.82171270 (to ZL)Public Service Platform for Artificial In telligence Screening and Auxiliary Diagnosis for the Medical and Health Industry,Ministry of Industry and Information Technology of the People's Republic of China,No.2020-0103-3-1 (to ZL)+3 种基金the Natural Science Foundation of Beijing,No.Z200016 (to ZL)Beijing Talents Project,No.2018000021223ZK03 (to ZL)Beijing Municipal Committee of Science and Technology,No.Z201 100005620010 (to ZL)CAMS Innovation Fund for Medical Sciences,No.2019-I2M-5-029 (to YongW)。
文摘Inflammation is closely related to stroke prognosis, and high inflammation status leads to poor functional outcome in stroke. DNA methylation is involved in the pathogenesis and prognosis of stroke. However, the effect of DNA methylation on stroke at high levels of inflammation is unclear. In this study, we constructed a hyperinflammatory cerebral ischemia mouse model and investigated the effect of hypomethylation and hypermethylation on the functional outcome. We constructed a mouse model of transient middle cerebral artery occlusion and treated the mice with lipopolysaccharide to induce a hyperinflammatory state. To investigate the effect of DNA methylation on stroke, we used small molecule inhibitors to restrain the function of key DNA methylation and demethylation enzymes. 2,3,5-Triphenyltetrazolium chloride staining, neurological function scores, neurobehavioral tests, enzyme-linked immunosorbent assay, quantitative reverse transcription PCR and western blot assay were used to evaluate the effects after stroke in mice. We assessed changes in the global methylation status by measuring DNA 5-mc and DNA 5-hmc levels in peripheral blood after the use of the inhibitor. In the group treated with the DNA methylation inhibitor, brain tissue 2,3,5-triphenyltetrazolium chloride staining showed an increase in infarct volume, which was accompanied by a decrease in neurological scores and worsening of neurobehavioral performance. The levels of inflammatory factors interleukin 6 and interleukin-1 beta in ischemic brain tissue and plasma were elevated, indicating increased inflammation. Related inflammatory pathway exploration showed significant overactivation of nuclear factor kappa B. These results suggested that inhibiting DNA methylation led to poor functional outcome in mice with high inflammation following stroke. Further, the effects were reversed by inhibition of DNA demethylation. Our findings suggest that DNA methylation regulates the inflammatory response in stroke and has an important role in the functional outcome of hyperinflammatory stroke.
文摘Background:Recently,ultra-processed foods(UPFs)have attracted considerable attention,leading to numerous studies worldwide.Scientometrics is currently gaining popularity among scientific communities,offering advantage of providing critical references to scholars of specific fields.Methods:This scientometric study aimed to analyze trends and hotspots of UPF research using English articles or reviews related to UPFs retrieved from the Web of Science Core Collection on March 5,2023.Two independent researchers selected the identified records on titles,abstracts,and author’s keywords,and the data were analyzed using R-bibliometrix,CiteSpace,and VOSviewer.Results:A total of 1018 publications(901 articles and 117 reviews)published from 2010 to 2023 were included.The result showed a significant increase in UPF publications over the past decades.Brazil published the highest number of papers(n=473),with over half of the top 10 active institutions were located in Brazil.The University of São Paulo contributed the most publications(n=206)and the 10 most productive authors belonged to this institution,with Monteiro CA and Levy RB contributing the most publications.The main research foundations included the NOVA system,the definition of UPFs,the prevalence of UPFs,and the impact of UPFs on dietary quality and health status.The main research frontiers included topics such as“systematic review,”“NOVA food classification,”“COVID-19,”“diabetes,”“pregnancy,”“food addiction,”“warning labels,”“plant-based diet,”and“commercial determinants of health”.Conclusions:This study provided a comprehensive overview of development trends and research hotspots of global UPF studies.
基金supported by Basic Science Research Program through the National Natural Science Foundation of China Youth Program(No.NNSF-81603496)
文摘Objective: To summarize and evaluate the evidence of guidelines and systematic reviews(SRs) of nonpharmacological interventions for mild cognitive impairment(MCI) to support the development of future guidelines and clinical decisions for MCI patients.Methods: Scottish Intercollegiate Guideline Network(SIGN), National Institute for Health and Clinical Excellence(NICE), American Academy of Neurology(AAN), Registered Nurses Association of Ontario(RNAO), Web of Science, PubMed, Cochrane Library, CNAHL, VIP, China National Knowledge Infrastructure(CNKI), and Wanfang Database were searched for relevant publications, including guidelines and SRs, from January 2014 to March 2019. Two authors independently screened articles, extracted data, and assessed the publications for adherence to the inclusion criteria. Appraisal of Guidelines for Research and Evaluation(AGREE II) was used to assess the quality of the guidelines, and Assessment of Multiple Systematic Reviews(AMSTAR 2) was used to assess the quality of SRs. In addition, the Grading of Recommendations Assessment, Development and Evaluation(GRADE) approach was used to evaluate the quality of outcomes.Results: Thirty-two articles were retrieved, including 1 guideline and 31 SRs. Fourteen SRs of physical exercise for MCI, six articles describing cognitive interventions, four articles describing acupuncture, and seven articles assessing dietary interventions(including four articles employing a Mediterranean diet, one article using vitamin B supplementation, and two articles assessing the effects of tea, coffee, and caffeine) were included. The quality of the articles was very low for 4(13%), low for 10(32%), and moderate for 17(55%).Conclusions: Based on the evidence available to date, nonpharmacological interventions may improve the current cognitive function of persons with MCI. In particular, physical exercise, cognitive interventions, and acupuncture exerted promising effects. However, due to the limited number and quality of the included publications, additional high-quality reviews are needed to further confirm.
文摘[目的]获取与轻度认知功能障碍(mild cognitive impairment,MCI)病人或照护者真实体验(患病后、接受某一干预后等)有关的近年来的系统评价或Meta整合,对其进行证据总结,以期为MCI病人相关指南推荐意见的制定提供参考性建议。[方法]在Web of Science、PubMed、CINAHL、Cochrane、中国知网、维普中文科技期刊、万方等中英文数据库进行系统性检索,采用JBI循证卫生保健中心(2008)的评价工具对质性研究原始研究进行质量评价,必要时采用CERQual对证据体进行分级。通过反复阅读最佳证据的内容,提取相应信息。[结果]共纳入2篇质性研究系统评价,该系统评价纳入的原始研究质量均较高,研究表明多数病人或照护者对辅助或改善认知能力相关技术保持积极的态度,但在实际应用时仍有诸多考虑。此外,经CERQual评价后,有中等质量的证据显示,MCI病人对生活呈现新的认识,且对MCI相关的医学知识欠缺深入的了解。[结论]本研究总结MCI病人或照护者真实体验的最佳证据,为临床护理专家或政策制定者提供循证支持。但目前最佳证据的数量及证据力度尚显薄弱,可能对研究结果的诠释带来影响,未来期待更多关于MCI病人或照护者真实体验的质性研究和更高质量的Meta整合。
文摘[目的]获取与轻度认知功能障碍(MCI)运动干预相关的指南、系统评价或Meta分析,对其进行证据总结与评价,以期为MCI病人相关指南推荐意见的制定提供参考性建议。[方法]计算机检索加拿大安大略注册护士协会(Registered Nurses Association of Ontario,RNAO)、美国神经病协会(American Academy of Neurology,AAN)、英国国家卫生与临床优化研究所(National Institute for Health and Clinical Excellence,NICE)、苏格兰院际指南网(Scottish Intercollegiate Guideline Network,SIGN)、PubMed、Cochrane library、Web of science、CINAHL、CNKI、万方及维普数据库获得相关指南及系统评价,检索时限从2014年1月—2018年2月。采用AMSTAR 2评价工具对文献质量进行评价,必要时应用GRADE工具对证据体进行证据分级,针对指南的质量评价采用AGREEⅡ标准进行评价。[结果]最终纳入1篇指南,12篇系统评价/Meta分析,AMASTA 2评价结果显示,9篇文献为中等质量。GRADE证据质量评价显示,3个证据体证据质量为高质量,11个证据体为中等质量,10个证据体为低质量,3个证据体为极低质量。[结论]运动干预具有较好的疗效,可作为MCI病人的临床辅助治疗措施,但运动干预对MCI病人干预效果系统评价的方法学质量总体不高,其真实效果有待大样本、高质量的研究进一步探讨。
文摘[目的]总结与评价非药物干预治疗轻度认知功能障碍(mild cognitive impairment,MCI)的指南及系统评价,为改善中老年人认知功能、延缓向老年性痴呆的转化提供依据。[方法]计算机检索加拿大安大略注册护士协会(Registered Nurses Association of Ontario,RNAO)、美国神经病协会(American Academy of Neurology,AAN)、英国国家卫生与临床优化研究所(National Institute for Health and Clinical Excellence,NICE)、苏格兰院际指南网(Scottish Intercollegiate Guideline Network,SIGN)、Cochrane Library、PubMed、Web of Science、CINAHL、中国知网(CNKI)、万方及维普数据库获得相关指南及系统评价。[结果]共纳入30篇文献,1篇指南,重点介绍了对MCI病人进行评估和筛查、MCI病人药物及非药物治疗;29篇系统评价,包括4篇针刺疗法治疗MCI系统评价,7篇饮食干预治疗MCI系统评价(包括4篇地中海饮食,1篇补充维生素B,3篇咖啡摄入),12篇运动干预治疗MCI系统评价(包括太极运动、有氧运动等),4篇认知干预治疗MCI系统评价,2篇质性研究系统评价。[结论]非药物干预治疗MCI研究有重要意义,且大量研究显示有较好疗效,可作为MCI病人的临床辅助治疗措施。
