OBJECTIVE: To investigate the effects of Ningmitai capsule combined with sertraline on patients with premature ejaculation(PE) and an increased anterior-posterior diameter(APD) of the seminal vesicles(SVs).METHODS: Si...OBJECTIVE: To investigate the effects of Ningmitai capsule combined with sertraline on patients with premature ejaculation(PE) and an increased anterior-posterior diameter(APD) of the seminal vesicles(SVs).METHODS: Sixty men with acquired PE were enrolled and randomly divided into two groups. The combined group was treated with Ningmitai capsule and sertraline, while the control group was treated with sertraline alone. Main outcomes were measured using the premature ejaculation diagnostic tool(PEDT), APD of SVs, and Clinical Global Impression of Change questionnaire and compared before and after 3 months of treatment.RESULTS: Comparing after treatment with before treatment outcomes within each group, the PEDT score was significantly reduced in the combined group(12.1 ± 2.5 vs 8.6 ± 3.2, P < 0.001, respectively) and control group(12.9 ± 2.6 vs 10.3 ± 1.6, P <0.001, respectively). Furthermore, the PEDT score after treatment was significantly lower in the combined compared with control group(8.6 ± 3.2 vs10.3 ± 1.6, P = 0.011, respectively). The APD of SVs in the combined group was significantly decreased after treatment [(10.8 ± 2.4) vs(12.9 ± 2.2) mm, P =0.001], while the APD of SVs in the control group was equivalent before and after treatment. The treatment response rate was not significantly higher in the combined compared with control group.CONCLUSION: These results indicated that the effect of Ningmitai capsule combined with sertraline was better than that of sertraline alone for the treatment of PE patients exhibiting an increased APD of SVs. The therapeutic effect found for the combined treatment may be due to antibacterial and anti-inflammatory activity reported for Ningmitai capsule,and may suggest that seminal vesiculitis is a potential pathophysiological factor in acquired PE.展开更多
目的研究自制微流控芯片优选人类精子的效果。方法自行构建微流控芯片装置。收集男性不育患者的精液样本:(1)分别置于无毒灭菌离心管与微流控芯片中1h、2h、3h、4h,比较各时间点两组精子活力的变化;(2)分别用微流控芯片优选精子40min、6...目的研究自制微流控芯片优选人类精子的效果。方法自行构建微流控芯片装置。收集男性不育患者的精液样本:(1)分别置于无毒灭菌离心管与微流控芯片中1h、2h、3h、4h,比较各时间点两组精子活力的变化;(2)分别用微流控芯片优选精子40min、60min、80min,比较各时间点优选前后各项精子参数的变化;(3)分别用微流控芯片、上游法和密度梯度离心法优选精子,比较三种方法优选前后各项精子参数的变化。结果(1)静置1h、2h、3h、4h后,无毒灭菌离心管与微流控芯片中的精子活动率与前向运动精子百分率均无显著性差异(P>0.05)。(2)与优选前相比,微流控芯片优选精子40min、60min、80min后,精子活动率(36.54%vs 96.55%、97.92%、88.12%)和前向运动精子百分率(28.01%vs 83.04%、83.01%、81.25%)均显著上升(P<0.01),而精子浓度(47.11×10^(6)/m L vs 1.68×10^(6)/mL、3.81×10^(6)/mL、4.02×10^(6)/m L)均显著降低(P<0.01),但60min与80min组的精子浓度显著高于40min组(P<0.05);(3)与上游法和密度梯度离心法相比,微流控芯片优选后的精子活动率(89.58%vs 70.76%、71.89%)和前向运动精子百分率(82.35%vs 51.52%、45.99%)显著提升,而精子浓度(4.09×10^(6)/mL vs 17.06×10^(6)/mL、60.33×10^(6)/m L)和DFI(15.53%vs 30.08%、40.52%)显著降低(P<0.05)。结论微流控芯片对精子无毒并能优选出高质量精子,与两种常规精子优选方法相比,其优选后的精子DNA损伤较小,更适合于辅助生殖技术IVF/ICSI中使用。展开更多
基金Supported by a Grant from the National Key Basic Research Program of China(No.2013CB945204)a Research and Development Project for Young Doctors in Reproductive Medicine from the Chinese Medical Association Specialized Fund for Clinical Medical Research(No.16020380654)
文摘OBJECTIVE: To investigate the effects of Ningmitai capsule combined with sertraline on patients with premature ejaculation(PE) and an increased anterior-posterior diameter(APD) of the seminal vesicles(SVs).METHODS: Sixty men with acquired PE were enrolled and randomly divided into two groups. The combined group was treated with Ningmitai capsule and sertraline, while the control group was treated with sertraline alone. Main outcomes were measured using the premature ejaculation diagnostic tool(PEDT), APD of SVs, and Clinical Global Impression of Change questionnaire and compared before and after 3 months of treatment.RESULTS: Comparing after treatment with before treatment outcomes within each group, the PEDT score was significantly reduced in the combined group(12.1 ± 2.5 vs 8.6 ± 3.2, P < 0.001, respectively) and control group(12.9 ± 2.6 vs 10.3 ± 1.6, P <0.001, respectively). Furthermore, the PEDT score after treatment was significantly lower in the combined compared with control group(8.6 ± 3.2 vs10.3 ± 1.6, P = 0.011, respectively). The APD of SVs in the combined group was significantly decreased after treatment [(10.8 ± 2.4) vs(12.9 ± 2.2) mm, P =0.001], while the APD of SVs in the control group was equivalent before and after treatment. The treatment response rate was not significantly higher in the combined compared with control group.CONCLUSION: These results indicated that the effect of Ningmitai capsule combined with sertraline was better than that of sertraline alone for the treatment of PE patients exhibiting an increased APD of SVs. The therapeutic effect found for the combined treatment may be due to antibacterial and anti-inflammatory activity reported for Ningmitai capsule,and may suggest that seminal vesiculitis is a potential pathophysiological factor in acquired PE.
文摘目的研究自制微流控芯片优选人类精子的效果。方法自行构建微流控芯片装置。收集男性不育患者的精液样本:(1)分别置于无毒灭菌离心管与微流控芯片中1h、2h、3h、4h,比较各时间点两组精子活力的变化;(2)分别用微流控芯片优选精子40min、60min、80min,比较各时间点优选前后各项精子参数的变化;(3)分别用微流控芯片、上游法和密度梯度离心法优选精子,比较三种方法优选前后各项精子参数的变化。结果(1)静置1h、2h、3h、4h后,无毒灭菌离心管与微流控芯片中的精子活动率与前向运动精子百分率均无显著性差异(P>0.05)。(2)与优选前相比,微流控芯片优选精子40min、60min、80min后,精子活动率(36.54%vs 96.55%、97.92%、88.12%)和前向运动精子百分率(28.01%vs 83.04%、83.01%、81.25%)均显著上升(P<0.01),而精子浓度(47.11×10^(6)/m L vs 1.68×10^(6)/mL、3.81×10^(6)/mL、4.02×10^(6)/m L)均显著降低(P<0.01),但60min与80min组的精子浓度显著高于40min组(P<0.05);(3)与上游法和密度梯度离心法相比,微流控芯片优选后的精子活动率(89.58%vs 70.76%、71.89%)和前向运动精子百分率(82.35%vs 51.52%、45.99%)显著提升,而精子浓度(4.09×10^(6)/mL vs 17.06×10^(6)/mL、60.33×10^(6)/m L)和DFI(15.53%vs 30.08%、40.52%)显著降低(P<0.05)。结论微流控芯片对精子无毒并能优选出高质量精子,与两种常规精子优选方法相比,其优选后的精子DNA损伤较小,更适合于辅助生殖技术IVF/ICSI中使用。
