Background:Piwi-interacting RNAs(piRNAs)are a type of non-coding RNAs,initially identified in germ cells in 2006,known to bind to the Piwi family proteins.Accumulating studies indicate their importance in genome stabi...Background:Piwi-interacting RNAs(piRNAs)are a type of non-coding RNAs,initially identified in germ cells in 2006,known to bind to the Piwi family proteins.Accumulating studies indicate their importance in genome stability,epigenetics regulation,germ cell differentiation,and tumor development.Despite growing interest in piRNA research,there is a lack of comprehensive bibliometric studies on the subject.This study aims to analyze piRNA research trends from 2006 to 2023.Methods:The literature regarding piRNA was sourced from the Web of Science on April 25,2023.VOSviewer,CiteSpace and a bibliometric online website(https://bibliometric.com/app)were employed to perform bibliometric analysis.Network maps were constructed to evaluate the collaborations among countries,institutions,authors,journals,references,keywords,and research hot pots.Results:In this study,2549 literature were published across 464 countries and 6921 institutions,comprising 2010 articles and 539 reviews.The United States led in publication output(n=1011,39.66%),followed by China(635,24.91%).The University of Tokyo had the most publications among all institutions(n=100,3.92%),followed by the Chinese Academy of Sciences(n=86,3.37%).Among 631 published journals,Nucleic Acids Research was the most published journal(n=83,3.26%).Siomi Mikiko C published the most articles(n=58),with Aravin Alexei A as the most co-cited author.Analysis of term co-occurrence unveiled three highly interconnected clusters,including“piRNA biogenesis and function”,“cancer and regulation”,as well as“protein and species”.The research focus has transferred from male reproductive development to tumor progression.Conclusion:This bibliometric analysis offered a thorough overview of the current state of piRNA research,deepening understanding of the progress in this field over the last 17 years and providing a valuable reference for scholars engaged in piRNA studies.展开更多
The high temperature creep behavior of carbon nanotube(CNT)/alumina was mediated by the surface chemical functionalization used for synthesis of composite powders. Non-covalent functionalized carbon nanotubes make com...The high temperature creep behavior of carbon nanotube(CNT)/alumina was mediated by the surface chemical functionalization used for synthesis of composite powders. Non-covalent functionalized carbon nanotubes make composites ductile, but covalent approach leads composites that are creep-resistant. Oxygen vacancy mechanism is proposed to account for this mediation effect in this communication.展开更多
为了进一步降低社会交通事故伤亡率,C-NCAP引入了移动式渐进可变形碰撞测试工况(MPDB),用以评估车辆碰撞兼容性。主要是针对MPDB工况与车对车碰撞事故的相关性问题及车身碰撞兼容性优化方法进行研究。选取某SUV车型作为“基准车”。选...为了进一步降低社会交通事故伤亡率,C-NCAP引入了移动式渐进可变形碰撞测试工况(MPDB),用以评估车辆碰撞兼容性。主要是针对MPDB工况与车对车碰撞事故的相关性问题及车身碰撞兼容性优化方法进行研究。选取某SUV车型作为“基准车”。选取另一辆车作为“子弹车”,基于压溃理论对其进行MPDB工况仿真分析及优化,得到三种碰撞兼容性评分状态下的“子弹车”,与“基准车”进行车对车碰撞仿真,对比发现MPDB工况与实车对撞(Car to Car)工况下车辆碰撞兼容性表现具有较强的相关性。展开更多
Objective The protein interacting with C kinase 1(PICK1)plays a critical role in vesicle trafficking,and its deficiency in sperm cells results in abnormal vesicle trafficking from Golgi to acrosome,which eventually di...Objective The protein interacting with C kinase 1(PICK1)plays a critical role in vesicle trafficking,and its deficiency in sperm cells results in abnormal vesicle trafficking from Golgi to acrosome,which eventually disrupts acrosome formation and leads to male infertility.Methods An azoospermia sample was filtered,and the laboratory detection and clinical phenotype indicated typical azoospermia in the patient.We sequenced all of the exons in the PICK1 gene and found that there was a novel homozygous variant in the PICK1 gene,c.364delA(p.Lys122SerfsX8),and this protein structure truncating variant seriously affected the biological function.Then we constructed a PICK1 knockout mouse model using clustered regularly interspaced short palindromic repeat cutting technology(CRISPRc).Results The sperm from PICK1 knockout mice showed acrosome and nucleus abnormalities,as well as dysfunctional mitochondrial sheath formation.Both the total sperm and motility sperm counts were decreased in the PICK1 knockout mice compared to wild-type mice.Moreover,the mitochondrial dysfunction was verified in the mice.These defects in the male PICK1 knockout mice may have eventually led to complete infertility.Conclusion The c.364delA novel variant in the PICK1 gene associated with clinical infertility,and pathogenic variants in the PICK1 may cause azoospermia or asthenospermia by impairing mitochondrial function in both mice and humans.展开更多
Complicated and large acetabular bone defects present the main challenges and difficulty in the revision of total hip arthroplasty(THA).This study aimed to explore the advantages of three-dimensional(3D)printing techn...Complicated and large acetabular bone defects present the main challenges and difficulty in the revision of total hip arthroplasty(THA).This study aimed to explore the advantages of three-dimensional(3D)printing technology in the reconstruction of such acetabular bone defects.We retrospectively analyzed the prognosis of four severe bone defects around the acetabulum in three patients who were treated using 3D printing technology.Reconstruction of bone defect by conventional methods was difficult in these patients.In this endeavor,we used radiographic methods,related computer software such as Materialise's interactive medical image control system and Siemens NX software,and actual surgical experience to estimate defect volume,prosthesis stability,and installation accuracy,respectively.Moreover,a Harris hip score was obtained to evaluate limb function.It was found that bone defects could be adequately reconstructed using a 3D printing prosthesis,and its stability was reliable.The Harris hip score indicated a very good functional recovery in all three patients.In conclusion,3D printing technology had a good therapeutic effect on both complex and large bone defects in the revision of THA.It was able to achieve good curative effects in patients with large bone defects.展开更多
Severe capacity fading and poor high rate performance of lithium sulfur(Li–S) battery caused by "shuttle effect" and low conductivity of sulfur hampers its further developments and applications. Li_4Ti_5O_(...Severe capacity fading and poor high rate performance of lithium sulfur(Li–S) battery caused by "shuttle effect" and low conductivity of sulfur hampers its further developments and applications. Li_4Ti_5O_(12) (LTO)possesses high lithium ion conductivity, and it is also can be used as an active adsorbent for polysulfide. Herein, fine LTO particle coated carbon nanofibers(CNF) were prepared and a conductive network both for electron and lithium ion was built, which can greatly promote the electrochemical conversion of polysulfide and improve the rate performance of Li–S batteries. Meanwhile, a quantity of adsorption sites is constructed by defects of the surface of LTO-CNF membrane to effectively immobilize polysulfide. The multifunctional LTO-CNF interlayer could impede the shuttle effect and enhance comprehensive electrochemical performance of Li–S batteries, especially high rate performance. With such LTO-CNF interlayer,the Li–S battery presents a specific capacity of 641.9 mAh/g at 5 C rate. After 400 cycles at 1 C, a capacity of 618.0 mAh/g is retained. This work provides a feasible strategy to achieve high performance of Li–S battery for practical utilization.展开更多
Spinocerebellar ataxia type 3/Machado-Joseph disease （SCA3/MJD） is a progressive, currently untreatable and ultimately fatal ataxic disorder that belongs to the group of neurological disorders known as CAG-repeat or...Spinocerebellar ataxia type 3/Machado-Joseph disease （SCA3/MJD） is a progressive, currently untreatable and ultimately fatal ataxic disorder that belongs to the group of neurological disorders known as CAG-repeat or polyglutamine diseases. Here, we present the first prenatal diagnosis of SCA3/MJD in China's Mainland in a woman who was known to carry an expanded CAG-trinucleotide repeat in the MJD1 gene. After evaluating motivation and psychological tolerance of the couple, amniocentesis was performed after 14 weeks of gestation. Polymerase chain reactions followed by T-vector cloning and direct sequencing were employed to evaluate the CAG-repeat number of the fetal MJD1 gene. We identified a truncated CAG expansion of 78 repeats in the MJD1 gene of the fetus compared with 81 repeats in his mother.展开更多
基金This study was funded by grants from the Zhejiang Province Traditional Chinese Medicine Science and Technology Project(2023ZL056)the Foundation Project of Zhejiang Chinese Medical University(2022JKJNTZ16).
文摘Background:Piwi-interacting RNAs(piRNAs)are a type of non-coding RNAs,initially identified in germ cells in 2006,known to bind to the Piwi family proteins.Accumulating studies indicate their importance in genome stability,epigenetics regulation,germ cell differentiation,and tumor development.Despite growing interest in piRNA research,there is a lack of comprehensive bibliometric studies on the subject.This study aims to analyze piRNA research trends from 2006 to 2023.Methods:The literature regarding piRNA was sourced from the Web of Science on April 25,2023.VOSviewer,CiteSpace and a bibliometric online website(https://bibliometric.com/app)were employed to perform bibliometric analysis.Network maps were constructed to evaluate the collaborations among countries,institutions,authors,journals,references,keywords,and research hot pots.Results:In this study,2549 literature were published across 464 countries and 6921 institutions,comprising 2010 articles and 539 reviews.The United States led in publication output(n=1011,39.66%),followed by China(635,24.91%).The University of Tokyo had the most publications among all institutions(n=100,3.92%),followed by the Chinese Academy of Sciences(n=86,3.37%).Among 631 published journals,Nucleic Acids Research was the most published journal(n=83,3.26%).Siomi Mikiko C published the most articles(n=58),with Aravin Alexei A as the most co-cited author.Analysis of term co-occurrence unveiled three highly interconnected clusters,including“piRNA biogenesis and function”,“cancer and regulation”,as well as“protein and species”.The research focus has transferred from male reproductive development to tumor progression.Conclusion:This bibliometric analysis offered a thorough overview of the current state of piRNA research,deepening understanding of the progress in this field over the last 17 years and providing a valuable reference for scholars engaged in piRNA studies.
基金supported by grants from ‘hundred Talents Programme’ of The Chinese Academy of Sciences (KJCX2-EW-H06)Qianjiang excellent project (2011R10020) and Zhejiang NSF (Y4ll0376)+2 种基金National Natural Science Foundation of China (Grant No. 51172248)State Key Laboratory of Porous Metal Materials (PMM-SKL-1-2013)NSF-CMMI (grant number CMMI-0700272)
文摘The high temperature creep behavior of carbon nanotube(CNT)/alumina was mediated by the surface chemical functionalization used for synthesis of composite powders. Non-covalent functionalized carbon nanotubes make composites ductile, but covalent approach leads composites that are creep-resistant. Oxygen vacancy mechanism is proposed to account for this mediation effect in this communication.
文摘为了进一步降低社会交通事故伤亡率,C-NCAP引入了移动式渐进可变形碰撞测试工况(MPDB),用以评估车辆碰撞兼容性。主要是针对MPDB工况与车对车碰撞事故的相关性问题及车身碰撞兼容性优化方法进行研究。选取某SUV车型作为“基准车”。选取另一辆车作为“子弹车”,基于压溃理论对其进行MPDB工况仿真分析及优化,得到三种碰撞兼容性评分状态下的“子弹车”,与“基准车”进行车对车碰撞仿真,对比发现MPDB工况与实车对撞(Car to Car)工况下车辆碰撞兼容性表现具有较强的相关性。
基金supported by grants from Zhejiang Provincial Natural Science Foundation of China(No.LQ21H200007)National Natural Science Foundation of China(No.82202605,No.81772664 and No.82172363)+1 种基金Zhejiang Provincial People’s Hospital Excellent Scientific Research Start-up Fundation of China(No.ZRY2019C008)Hangzhou Medical College Fundamental Scientific Research Project of China(No.KYQN202116).
文摘Objective The protein interacting with C kinase 1(PICK1)plays a critical role in vesicle trafficking,and its deficiency in sperm cells results in abnormal vesicle trafficking from Golgi to acrosome,which eventually disrupts acrosome formation and leads to male infertility.Methods An azoospermia sample was filtered,and the laboratory detection and clinical phenotype indicated typical azoospermia in the patient.We sequenced all of the exons in the PICK1 gene and found that there was a novel homozygous variant in the PICK1 gene,c.364delA(p.Lys122SerfsX8),and this protein structure truncating variant seriously affected the biological function.Then we constructed a PICK1 knockout mouse model using clustered regularly interspaced short palindromic repeat cutting technology(CRISPRc).Results The sperm from PICK1 knockout mice showed acrosome and nucleus abnormalities,as well as dysfunctional mitochondrial sheath formation.Both the total sperm and motility sperm counts were decreased in the PICK1 knockout mice compared to wild-type mice.Moreover,the mitochondrial dysfunction was verified in the mice.These defects in the male PICK1 knockout mice may have eventually led to complete infertility.Conclusion The c.364delA novel variant in the PICK1 gene associated with clinical infertility,and pathogenic variants in the PICK1 may cause azoospermia or asthenospermia by impairing mitochondrial function in both mice and humans.
基金This work is supported by National Key Research and Development Program of China(2016YFC1100600)the National Natural Science Foundation of China(81972058 and 81902194)the Multicenter Clinical Research Project of Shanghai Jiao Tong University School of Medicine(DLY201506).
文摘Complicated and large acetabular bone defects present the main challenges and difficulty in the revision of total hip arthroplasty(THA).This study aimed to explore the advantages of three-dimensional(3D)printing technology in the reconstruction of such acetabular bone defects.We retrospectively analyzed the prognosis of four severe bone defects around the acetabulum in three patients who were treated using 3D printing technology.Reconstruction of bone defect by conventional methods was difficult in these patients.In this endeavor,we used radiographic methods,related computer software such as Materialise's interactive medical image control system and Siemens NX software,and actual surgical experience to estimate defect volume,prosthesis stability,and installation accuracy,respectively.Moreover,a Harris hip score was obtained to evaluate limb function.It was found that bone defects could be adequately reconstructed using a 3D printing prosthesis,and its stability was reliable.The Harris hip score indicated a very good functional recovery in all three patients.In conclusion,3D printing technology had a good therapeutic effect on both complex and large bone defects in the revision of THA.It was able to achieve good curative effects in patients with large bone defects.
基金supported by the National Key Basic Research Program of China (2014CB932400)the National Natural Science Foundation of China (51672156 and 51232005)+3 种基金Guangdong special support program (2015TQ01N401)Guangdong Province Technical Plan Project (2017B010119001 and 2017B090907005)Dongguan City (2015509119213)Shenzhen Technical Plan Project (JCYJ20170817161221958, JCYJ20170412170706047, JCYJ20170307153806471, and GJHS20170314165324888)
文摘Severe capacity fading and poor high rate performance of lithium sulfur(Li–S) battery caused by "shuttle effect" and low conductivity of sulfur hampers its further developments and applications. Li_4Ti_5O_(12) (LTO)possesses high lithium ion conductivity, and it is also can be used as an active adsorbent for polysulfide. Herein, fine LTO particle coated carbon nanofibers(CNF) were prepared and a conductive network both for electron and lithium ion was built, which can greatly promote the electrochemical conversion of polysulfide and improve the rate performance of Li–S batteries. Meanwhile, a quantity of adsorption sites is constructed by defects of the surface of LTO-CNF membrane to effectively immobilize polysulfide. The multifunctional LTO-CNF interlayer could impede the shuttle effect and enhance comprehensive electrochemical performance of Li–S batteries, especially high rate performance. With such LTO-CNF interlayer,the Li–S battery presents a specific capacity of 641.9 mAh/g at 5 C rate. After 400 cycles at 1 C, a capacity of 618.0 mAh/g is retained. This work provides a feasible strategy to achieve high performance of Li–S battery for practical utilization.
基金grants from the National Science and Technology Pillar Program in the Eleventh Five-year Plan Period, No. 2006BAI05A07the Major State Basic Research Development Program of China (973 Program), No. 2006cb500700+5 种基金the National Key Technologies Research and Development Program of China, No. 2004BA720A03the National Natural Science Foundation of China, No. 30871354, 30710303061 and 30470619the Key Project in the Natural Science Foundation of Hunan Province, No. 08JJ3048the Natural Science Foundation of Hunan Province, No. 11JJ5071the Science and Technology Planning Project of Hunan Province, No. 2009SK3172the Graduate Degree Thesis Innovation Foundation of Central South University, No. 2008yb030
文摘Spinocerebellar ataxia type 3/Machado-Joseph disease （SCA3/MJD） is a progressive, currently untreatable and ultimately fatal ataxic disorder that belongs to the group of neurological disorders known as CAG-repeat or polyglutamine diseases. Here, we present the first prenatal diagnosis of SCA3/MJD in China's Mainland in a woman who was known to carry an expanded CAG-trinucleotide repeat in the MJD1 gene. After evaluating motivation and psychological tolerance of the couple, amniocentesis was performed after 14 weeks of gestation. Polymerase chain reactions followed by T-vector cloning and direct sequencing were employed to evaluate the CAG-repeat number of the fetal MJD1 gene. We identified a truncated CAG expansion of 78 repeats in the MJD1 gene of the fetus compared with 81 repeats in his mother.
