目的评估残肝分数(remnant liver volume to total liver volume,%RLV)、残肝体积/标准肝体积(remnant liver volume to standard liver volume,RLV/SLV)、残肝体积/体重比率(remnant liver volume to body weight ratio,RLV/BW)、标准...目的评估残肝分数(remnant liver volume to total liver volume,%RLV)、残肝体积/标准肝体积(remnant liver volume to standard liver volume,RLV/SLV)、残肝体积/体重比率(remnant liver volume to body weight ratio,RLV/BW)、标准残肝体积(standard remnant liver volume,SRLV)预测肝细胞癌(hepatocellular carcinoma,HCC)患者行半肝切除术后发生肝功能衰竭(post-hepatectomy liver failure,PHLF)的效能。方法收集2013年9月至2016年8月于广西医科大学附属肿瘤医院行肝切除术的1446例乙型肝炎病毒相关性HCC患者(HBV-HCC),根据是否发生PHLF分为PHLF组和无PHLF组。采用ROC曲线分析%RLV、RLV/SLV、RLV/BW、SRLV预测术后肝功能衰竭的效能,并比较术后并发症发生率。结果本研究共纳入181例HBV-HCC患者,其中PHLF组22例,无PHLF组159例,两组患者%RLV、RLV/SLV、RLV/BW、SRLV比较差异有统计学意义(P<0.05)。ROC曲线分析显示,%RLV、RLV/SLV、RLV/BW、SRLV的AUC分别为0.77、0.91、0.84、0.91,SRLV预测术后PHLF的效能优于%RLV及RLV/BW,差异有统计学意义(P<0.05)。以SRLV截点值340 mL/m^2分组,SRLV>340 mL/m^2组及SRLV≤340 mL/m^2组患者术后重度并发症发生率差异有统计学意义(P<0.01)。结论SRLV及RLV/SLV较RLV/BW及%RLV在预测HCC患者接受半肝切除术后发生肝功能衰竭中具有更高的效能,当SRLV≤340 mL/m^2时患者术后肝功能衰竭及重度并发症发生率更高。展开更多
AIM:To characterize effect of astaxanthin(ASX)in Aspergillus fumigatus(A.fumigatus)induced keratitis in mouse model.METHODS:In vivo,fungal keratitis mouse model was established in C57BL/6 mice using A.fumigatus,follow...AIM:To characterize effect of astaxanthin(ASX)in Aspergillus fumigatus(A.fumigatus)induced keratitis in mouse model.METHODS:In vivo,fungal keratitis mouse model was established in C57BL/6 mice using A.fumigatus,followed by ASX or dimethyl sulfoxide(DMSO)treatment.Clinical responses were evaluated by clinical score and myeloperoxidase(MPO)assay.Inflammatory cytokines were assessed by reverse-transcription polymerase chain reaction(RT-PCR),Western blot,immunofluorescence,and enzyme-linked immuno sorbent assay(ELISA).RESULTS:In animal model,ASX improved corneal transparency and clinical response,suppressed the expression of inflammatory cytokine like IL-1β,TNF-α,and HMGB-1.Neutrophil levels have been shown to decrease in ASX-treated cornea by immunofluorescence and MPO.TLR2 and TLR4 levels were lower in ASX-treated group than DMSO-treated.CONCLUSION:ASX can suppress inflammatory response and reduce inflammatory cytokine production in mice model with A.fumigatus keratitis.展开更多
文摘目的评估残肝分数(remnant liver volume to total liver volume,%RLV)、残肝体积/标准肝体积(remnant liver volume to standard liver volume,RLV/SLV)、残肝体积/体重比率(remnant liver volume to body weight ratio,RLV/BW)、标准残肝体积(standard remnant liver volume,SRLV)预测肝细胞癌(hepatocellular carcinoma,HCC)患者行半肝切除术后发生肝功能衰竭(post-hepatectomy liver failure,PHLF)的效能。方法收集2013年9月至2016年8月于广西医科大学附属肿瘤医院行肝切除术的1446例乙型肝炎病毒相关性HCC患者(HBV-HCC),根据是否发生PHLF分为PHLF组和无PHLF组。采用ROC曲线分析%RLV、RLV/SLV、RLV/BW、SRLV预测术后肝功能衰竭的效能,并比较术后并发症发生率。结果本研究共纳入181例HBV-HCC患者,其中PHLF组22例,无PHLF组159例,两组患者%RLV、RLV/SLV、RLV/BW、SRLV比较差异有统计学意义(P<0.05)。ROC曲线分析显示,%RLV、RLV/SLV、RLV/BW、SRLV的AUC分别为0.77、0.91、0.84、0.91,SRLV预测术后PHLF的效能优于%RLV及RLV/BW,差异有统计学意义(P<0.05)。以SRLV截点值340 mL/m^2分组,SRLV>340 mL/m^2组及SRLV≤340 mL/m^2组患者术后重度并发症发生率差异有统计学意义(P<0.01)。结论SRLV及RLV/SLV较RLV/BW及%RLV在预测HCC患者接受半肝切除术后发生肝功能衰竭中具有更高的效能,当SRLV≤340 mL/m^2时患者术后肝功能衰竭及重度并发症发生率更高。
基金Supported by the National Natural Science Foundation of China(No.81870632)Youth Project of Natural Science Foundation of ghandong Province(No.ZR2019BH004).
文摘AIM:To characterize effect of astaxanthin(ASX)in Aspergillus fumigatus(A.fumigatus)induced keratitis in mouse model.METHODS:In vivo,fungal keratitis mouse model was established in C57BL/6 mice using A.fumigatus,followed by ASX or dimethyl sulfoxide(DMSO)treatment.Clinical responses were evaluated by clinical score and myeloperoxidase(MPO)assay.Inflammatory cytokines were assessed by reverse-transcription polymerase chain reaction(RT-PCR),Western blot,immunofluorescence,and enzyme-linked immuno sorbent assay(ELISA).RESULTS:In animal model,ASX improved corneal transparency and clinical response,suppressed the expression of inflammatory cytokine like IL-1β,TNF-α,and HMGB-1.Neutrophil levels have been shown to decrease in ASX-treated cornea by immunofluorescence and MPO.TLR2 and TLR4 levels were lower in ASX-treated group than DMSO-treated.CONCLUSION:ASX can suppress inflammatory response and reduce inflammatory cytokine production in mice model with A.fumigatus keratitis.