It is a severe challenge to construct 3D scaf- folds which hold controllable pore structure and similar morphology of the natural extracellular matrix （ECM）. In this study, a compound technology is proposed by com- ...It is a severe challenge to construct 3D scaf- folds which hold controllable pore structure and similar morphology of the natural extracellular matrix （ECM）. In this study, a compound technology is proposed by com- bining the 3D bioprinting and electrospinning process to fabricate 3D scaffolds, which are composed by orthogonal array gel microfibers in a grid-like arrangement and inter- calated by a nonwoven structure with randomly distributed polycaprolactone （PCL） nanofibers. Human adipose- derived stem cells （hASCs） are seeded on the hierarchical scaffold and cultured 21 d for in vitro study. The results of cells culturing show that the microfibers structure with controlled pores can allow the easy entrance of cells and the efficient diffusion of nutrients, and the nanofiber webs layered in the scaffold can significantly improve initial cell attachment and proliferation. The present work demon- strates that the hierarchical PCL/gel scaffolds consisting of controllable 3D architecture with interconnected pores and biomimetic nanofiber structures resembling the ECM can be designed and fabricated by the combination of 3D bioprinting and electrospinning to improve biological per- formance in tissue engineering applications.展开更多
基金supported by the National Natural Science Foundation of China(Grant No.51375292)
文摘It is a severe challenge to construct 3D scaf- folds which hold controllable pore structure and similar morphology of the natural extracellular matrix （ECM）. In this study, a compound technology is proposed by com- bining the 3D bioprinting and electrospinning process to fabricate 3D scaffolds, which are composed by orthogonal array gel microfibers in a grid-like arrangement and inter- calated by a nonwoven structure with randomly distributed polycaprolactone （PCL） nanofibers. Human adipose- derived stem cells （hASCs） are seeded on the hierarchical scaffold and cultured 21 d for in vitro study. The results of cells culturing show that the microfibers structure with controlled pores can allow the easy entrance of cells and the efficient diffusion of nutrients, and the nanofiber webs layered in the scaffold can significantly improve initial cell attachment and proliferation. The present work demon- strates that the hierarchical PCL/gel scaffolds consisting of controllable 3D architecture with interconnected pores and biomimetic nanofiber structures resembling the ECM can be designed and fabricated by the combination of 3D bioprinting and electrospinning to improve biological per- formance in tissue engineering applications.
