SERPINH1 promoted the proliferation and metastasis of colorectal cancer by activating PI3K/Akt/mTOR signaling pathway

BACKGROUND Serpin peptidase inhibitor clade H member 1(SERPINH1)was initially recognized as an oncogene implicated in various human malignancies.Nevertheless,the clinical relevance and functional implications of SERPINH1 in colorectal cancer(CRC)remain largely elusive.AIM To investigate the effects of SERPINH1 on CRC cells and its specific mechanism.METHODS Quantitative real-time polymerase chain reaction,western blotting analysis,The Cancer Genome Atlas data mining and immunohistochemistry were employed to examine SERPINH1 expression in CRC cell lines and tissues.A series of in-vitro assays were performed to demonstrate the function of SERPINH1 and its possible mechanisms in CRC.RESULTS SERPINH1 demonstrated elevated expression levels in both CRC cells and tissues,manifested at both mRNA and protein tiers.Elevated SERPINH1 levels correlated closely with advanced T stage,lymph node involvement,and distant metastasis,exhibiting a significant association with poorer overall survival among CRC patients.Subsequent investigations unveiled that SERPINH1 overexpression notably bolstered CRC cell proliferation,invasion,and migration in vitro,while conversely,SERPINH1 knockdown elicited the opposite effects.Gene set enrichment analysis underscored a correlation between SERPINH1 upregulation and genes associated with cell cycle regulation.Our findings underscored the capacity of heightened SERPINH1 levels to expedite G1/S phase cell cycle progression via phosphatidylinositol 3-kinase/AKT/mechanistic target of rapamycin pathway activation,thereby facilitating CRC cell invasion and migration.CONCLUSION These findings imply a crucial involvement of SERPINH1 in the advancement and escalation of CRC,potentially positioning it as a novel candidate for prognostic assessment and therapeutic intervention in CRC management.

Genetic diversity and offspring fitness in the red and white fruit color morphs of the wild strawberry Fragaria pentaphylla

果实颜色的多态性在植物界普遍存在,但是其维持机制尚不清楚。一些假说认为扩散者会偏好一些果色形态,但也有其它假说认为不同的果色形态具有各异的前扩散或后扩散适合度。我们比较分析了中国中南部野生二倍体五叶草莓(Fragaria pentaphylla)红白果型种群的遗传多样性、克隆多样性、交配系统、与抗冻性有关的形态特征以及子代的萌发、存活和种子产量等特征。五叶草莓红果型远远多于白果型,但基于观察到的有效等位基因数、香农信息指数、预期或观察到的杂合度数量等指标,红果型并未表现出更高的遗传多样性。分子方差分析(AMOVA)表明,大多数种群的遗传变异存在于红白果型内,而不是在红白果型间。红白果型的交配系统参数没有差异,且红白果型均未发现存在显著的双亲近交。红白果型间的基因流很高(N_(m)=6.89)。与白果型相比,红果型的种子的种子萌发约早2天,萌发率高出40%,但红白果型间的幼苗存活率和子代的种子产量没有差异。红果型总的扩散后适合度是白果型的2倍左右。红果型叶柄毛较多,但叶表面蜡质不多。综上所述,研究结果部分证据表明不同颜色形态的五叶草莓果实在扩散前后的适合度存在差异。因此,与扩散无关的适合度的差异部分解释了所有情况下果实颜色的多态性。