1 Introduction Obstructive atherosclerosis vascular disease remains one of the greatest public health threats in the world.Sur2 gical treatment to replace diseased blood vessels is usually done using major human allog...1 Introduction Obstructive atherosclerosis vascular disease remains one of the greatest public health threats in the world.Sur2 gical treatment to replace diseased blood vessels is usually done using major human allografts(veins or arteries)or synthetic prosthesis(PTFE,Dacron).However,these substitutes have not a good patency,because of the lack of endothelial cell s(ECs)layer,which prevent s thrombus formation.The challenge of tissue engineering vessel s i s to build2up blood/substitute interface near native vessels.In order to improve ECs adhesion,it is necessary to pre2 coat the intra2luminal vessel.Recently,a new surface modification technique arose,based on the alternate ad2 sorption of oppositely charged polyelectrolytes.Our objec2 tive was to favour the endothelialization of the cryo2pre2 served allografts,treated with a thin polyelectrolyte multi2 layered film,made of PSS(poly(sodium242styrenesul2 fonate))or PAH(poly(allylamine hydrochloride)).展开更多
1 Introduction Mesenchymal stem cells(MSCs)are multipotential stem cells which can be expanded in culture while still maintaining their undifferentiated state.They have the potential to differentiate into distinct mes...1 Introduction Mesenchymal stem cells(MSCs)are multipotential stem cells which can be expanded in culture while still maintaining their undifferentiated state.They have the potential to differentiate into distinct mesenchymal tissue cells,including chondrocytes.Thus,they are an attrac2 tive cell source for cartilage ti ssue engineering.In vitro high density micromass culture has been widely used for chondrogenesis induction.The objective of our study was to analyze viability and differentiation of hMSC in micro2 mass cultures.展开更多
文摘1 Introduction Obstructive atherosclerosis vascular disease remains one of the greatest public health threats in the world.Sur2 gical treatment to replace diseased blood vessels is usually done using major human allografts(veins or arteries)or synthetic prosthesis(PTFE,Dacron).However,these substitutes have not a good patency,because of the lack of endothelial cell s(ECs)layer,which prevent s thrombus formation.The challenge of tissue engineering vessel s i s to build2up blood/substitute interface near native vessels.In order to improve ECs adhesion,it is necessary to pre2 coat the intra2luminal vessel.Recently,a new surface modification technique arose,based on the alternate ad2 sorption of oppositely charged polyelectrolytes.Our objec2 tive was to favour the endothelialization of the cryo2pre2 served allografts,treated with a thin polyelectrolyte multi2 layered film,made of PSS(poly(sodium242styrenesul2 fonate))or PAH(poly(allylamine hydrochloride)).
文摘1 Introduction Mesenchymal stem cells(MSCs)are multipotential stem cells which can be expanded in culture while still maintaining their undifferentiated state.They have the potential to differentiate into distinct mesenchymal tissue cells,including chondrocytes.Thus,they are an attrac2 tive cell source for cartilage ti ssue engineering.In vitro high density micromass culture has been widely used for chondrogenesis induction.The objective of our study was to analyze viability and differentiation of hMSC in micro2 mass cultures.
