The infections in open fracture induce high morbidity worldwide.Thus,developing efficient anti-infective orthopedic devices is of great significance.In this work,we designed a kind of infection-responsive long-term an...The infections in open fracture induce high morbidity worldwide.Thus,developing efficient anti-infective orthopedic devices is of great significance.In this work,we designed a kind of infection-responsive long-term antibacterial bone plates.Through a facile and flexible volatilization method,a multi-aldehyde polysaccharide derivative,oxidized sodium alginate,was crosslinked with multi-amino compounds,gentamycin and gelatin,to fabricate a uniform coating on Ti bone plates via Schiff base reaction,which was followed by a secondary crosslinking process by glutaraldehyde.The double-crosslinked coating was stable under normal condition,and could responsively release gentamycin by the triggering of the acidic microenvironment caused by bacterial metabolism,owning to the pH-responsiveness of imine structure.The thickness of the coating was ranging from 22.0μm to 63.6μm.The coated bone plates(Ti-GOGs)showed infection-triggered antibacterial properties(>99%)and high biocompatibility.After being soaked for five months,it still possessed efficient antibacterial ability,showing its sustainable antibacterial performance.The in vivo anti-infection ability was demonstrated by an animal model of infection after fracture fixation(IAFF).At the early stage of IAFF,Ti-GOGs could inhibit the bacterial infection(>99%).Subsequently,Ti-GOGs could promote recovery of fracture of IAFF.This work provides a convenient and universal strategy for fabrication of various antibacterial orthopedic devices,which is promising to prevent and treat IAFF.展开更多
基金This work was supported by National Natural Science Foundation of China[Grant Nos.52122304,52073024 and 51873012]Beijing Outstanding Young Scientist Program[Grant No.BJJWZYJH01201910010024]Chinese Association of Rehabilitation Medicine Technology Development Project[Grant No.KFKT-2022-002].
文摘The infections in open fracture induce high morbidity worldwide.Thus,developing efficient anti-infective orthopedic devices is of great significance.In this work,we designed a kind of infection-responsive long-term antibacterial bone plates.Through a facile and flexible volatilization method,a multi-aldehyde polysaccharide derivative,oxidized sodium alginate,was crosslinked with multi-amino compounds,gentamycin and gelatin,to fabricate a uniform coating on Ti bone plates via Schiff base reaction,which was followed by a secondary crosslinking process by glutaraldehyde.The double-crosslinked coating was stable under normal condition,and could responsively release gentamycin by the triggering of the acidic microenvironment caused by bacterial metabolism,owning to the pH-responsiveness of imine structure.The thickness of the coating was ranging from 22.0μm to 63.6μm.The coated bone plates(Ti-GOGs)showed infection-triggered antibacterial properties(>99%)and high biocompatibility.After being soaked for five months,it still possessed efficient antibacterial ability,showing its sustainable antibacterial performance.The in vivo anti-infection ability was demonstrated by an animal model of infection after fracture fixation(IAFF).At the early stage of IAFF,Ti-GOGs could inhibit the bacterial infection(>99%).Subsequently,Ti-GOGs could promote recovery of fracture of IAFF.This work provides a convenient and universal strategy for fabrication of various antibacterial orthopedic devices,which is promising to prevent and treat IAFF.
