A heteropolysaccharide from Rhodiola rosea L.:preparation,purification and anti-tumor activities in H22-bearing mice

Numerous polysaccharides isolated from plants have been used to augment traditional drugs in the treatment of cancer.In order to explore the influence to hepatocellular carcinoma,a novel cold water-soluble polysaccharide was separated from Rhodiola rosea L.root(RLP)and then its structure and anti-cancer activities were tested.The chemical compositions and high performance gel permeation chromatography(HPGPC)results indicated that RLP was an acid heteropolysaccharide with the molecular weight of about1.15×10~6 Da.Furthermore,ion chromatography(IC),Fourier transform infrared(FT-IR)and nuclear magnetic resoance(NMR)further indicated that RLP was main composed of→2,4)-α-Rha(1→,→5)-α-L-Araf-(1→,α-D-Glu,→6)-β-D-Galp-(1→,β-D-Man and→4)-α-GalpA-(1→.In vivo antitumor activities of RLP were carried out by using H22 tumor-bearing mice model.The results shown that RLP(100 and 300 mg/kg)could inhibit tumor growth of H22 cells from 23.59%to 45.52%and protect thymuses and spleen without damage.In addition,according to cell cycle,AV-FITC/PI and JC-1,RLP could induce dose-dependent apopto sis of H22 cells via S phase arrested which was through a mitochondrial related pathway.Our data indicated that RLP has a broader application prospect in anti-tumor preparations.

Multifunctional modification of Fe_(3)O_(4) nanoparticles for diagnosis and treatment of diseases:A review

With the rapid improvements in nanomaterials and imaging technology,great progresses have been made in diagnosis and treatment of diseases during the pastdecades.Fe_(3)O_(4) magnetic nanoparticles(MNPs)with good biocompatibility and super-paramagnetic property are usually used as contrast agent for diagnosis of diseases inmagnetic resonance imaging(MRI).Currently,the combination of multiple imagingtechnologies has been considered as new tendency in diagnosis and treatment ofdiseases,which could enhance the accuracy and reliability of disease diagnosis andprovide new strategies for disease treatment.Therefore,novel contrast agents used formultifunctional imaging are urgently needed.Fe_(3)O_(4) MNPs are believed to be a potentialcandidate for construction of multifunctional platform in diagnosis and treatment ofdiseases.In recent years,there are a plethora of studies concerning the construction ofmultifunctional platform presented based on Fe_(3)O_(4) MNPs.In this review,we introducefabrication methods and modification strategies of Fe_(3)O_(4) MNPs,expecting greatimprovements for diagnosis and treatment of diseases in the future.