目的探讨弥漫大B细胞淋巴瘤(Diffuse large B-cell lymphoma,DLBCL)中TP53基因状态与免疫微环境相关因子CD4蛋白、CD8蛋白、白细胞介素-6(IL-6)水平之间的相关性,以及与各临床病理参数的关系。方法收集174例DLBCL标本,制备组织芯片。应...目的探讨弥漫大B细胞淋巴瘤(Diffuse large B-cell lymphoma,DLBCL)中TP53基因状态与免疫微环境相关因子CD4蛋白、CD8蛋白、白细胞介素-6(IL-6)水平之间的相关性,以及与各临床病理参数的关系。方法收集174例DLBCL标本,制备组织芯片。应用荧光原位杂交(Fluorescence in situ hybridization,FISH)检测TP53等位基因缺失情况,免疫组织化学法检测p53、CD4、CD8蛋白表达情况。收集伴TP53等位基因缺失及未缺失DLBCL患者的血液标本各10例,电化学发光免疫分析法检测IL-6水平。结果TP53等位基因缺失率为21.8%(38/174)。p53蛋白总阳性率为40.8%(71/174)。CD4、CD8蛋白的阳性率分别为1.72%(3/174)、81.03%(141/174)。发生TP53等位基因缺失的病例p53、CD8蛋白的阳性率更高(P<0.05),血清IL-6均值水平也更高(P<0.05)。TP53等位基因缺失、结外侵犯、疾病复发是DLBCL的不良预后独立影响因素(P<0.05)。结论TP53等位基因缺失的DLBCL病例中p53、CD8蛋白阳性率较高,血清IL-6水平升高,总生存率降低,表明TP53等位基因缺失影响DLBCL免疫微环境,是不良预后因子,可能成为免疫检测和/或治疗靶点。展开更多
To explore ways to improve the accuracy of quantitative analysis of samples in the micrometer to nanometer range of magnitudes,we adopted analytical transmission electron microscopy(AEM/EDS)for qualitative and quantit...To explore ways to improve the accuracy of quantitative analysis of samples in the micrometer to nanometer range of magnitudes,we adopted analytical transmission electron microscopy(AEM/EDS)for qualitative and quantitative analysis of pyrite materials.Additionally,the k factor of pyrite is calculated experimentally.To develop an appropriate non-standard quantitative analysis model for pyrite materials,the experimentally calculated k factor is compared with that estimated from the non-standard quantitative analytical model of the instrument software.The experimental findings demonstrate that the EDS attached to a TEM can be employed for precise quantitative analysis of micro-and nanoscale regions of pyrite materials.Furthermore,it serves as a reference for improving the results of the EDS quantitative analysis of other sulfides.展开更多
Globally,agricultural soils are considered as one of the most important sources of greenhouse gas(GHG)emissions.No-tillage(NT),one of the most admired ways of climate-smart agriculture,has been deemed to have co-benef...Globally,agricultural soils are considered as one of the most important sources of greenhouse gas(GHG)emissions.No-tillage(NT),one of the most admired ways of climate-smart agriculture,has been deemed to have co-benefit to mitigation of GHG emissions and sustainability for crop yield,however,the effect of NT on GHG emissions is controversial.This study analyzed the overall effects of NT on GHG emissions,as well as the moderators that significantly influenced the overall effects,of the wheat-based rotation cropping systems in China through meta-analysis.The results showed that the overall effect size of NT on methane(CH4)uptake,nitrous oxide(N2O)emission,and global warming potential(GWP)was 0.70(95%Confidence Interval(CI):0.21–1.19),-0.27(95%CI:-0.72–0.18),and-0.39(95%CI:-1.01–0.23),respectively.In temperate climate zones with alkaline soils,the nitrogen application rate of 120–240 kg/ha,NT could significantly reduce GHG emissions and GWP.However,the mitigation effect will be weakened along with NT duration,except for proper straw addition.Overall,NT has the potential to reduce GHG emissions from wheat-based rotation systems in China,but it is necessary to implement NT depending on local conditions,soil characteristics,and field management.展开更多
文摘目的探讨弥漫大B细胞淋巴瘤(Diffuse large B-cell lymphoma,DLBCL)中TP53基因状态与免疫微环境相关因子CD4蛋白、CD8蛋白、白细胞介素-6(IL-6)水平之间的相关性,以及与各临床病理参数的关系。方法收集174例DLBCL标本,制备组织芯片。应用荧光原位杂交(Fluorescence in situ hybridization,FISH)检测TP53等位基因缺失情况,免疫组织化学法检测p53、CD4、CD8蛋白表达情况。收集伴TP53等位基因缺失及未缺失DLBCL患者的血液标本各10例,电化学发光免疫分析法检测IL-6水平。结果TP53等位基因缺失率为21.8%(38/174)。p53蛋白总阳性率为40.8%(71/174)。CD4、CD8蛋白的阳性率分别为1.72%(3/174)、81.03%(141/174)。发生TP53等位基因缺失的病例p53、CD8蛋白的阳性率更高(P<0.05),血清IL-6均值水平也更高(P<0.05)。TP53等位基因缺失、结外侵犯、疾病复发是DLBCL的不良预后独立影响因素(P<0.05)。结论TP53等位基因缺失的DLBCL病例中p53、CD8蛋白阳性率较高,血清IL-6水平升高,总生存率降低,表明TP53等位基因缺失影响DLBCL免疫微环境,是不良预后因子,可能成为免疫检测和/或治疗靶点。
基金Funded by the International Science&Technology Cooperation Program of Hubei Province of China(No.2022EHB024)。
文摘To explore ways to improve the accuracy of quantitative analysis of samples in the micrometer to nanometer range of magnitudes,we adopted analytical transmission electron microscopy(AEM/EDS)for qualitative and quantitative analysis of pyrite materials.Additionally,the k factor of pyrite is calculated experimentally.To develop an appropriate non-standard quantitative analysis model for pyrite materials,the experimentally calculated k factor is compared with that estimated from the non-standard quantitative analytical model of the instrument software.The experimental findings demonstrate that the EDS attached to a TEM can be employed for precise quantitative analysis of micro-and nanoscale regions of pyrite materials.Furthermore,it serves as a reference for improving the results of the EDS quantitative analysis of other sulfides.
基金Under the auspices of the National Key Research and Development Program of China(No.2018YFD0300708-4)College Students’Innovative Entrepreneurial Training(No.202210476024)。
文摘Globally,agricultural soils are considered as one of the most important sources of greenhouse gas(GHG)emissions.No-tillage(NT),one of the most admired ways of climate-smart agriculture,has been deemed to have co-benefit to mitigation of GHG emissions and sustainability for crop yield,however,the effect of NT on GHG emissions is controversial.This study analyzed the overall effects of NT on GHG emissions,as well as the moderators that significantly influenced the overall effects,of the wheat-based rotation cropping systems in China through meta-analysis.The results showed that the overall effect size of NT on methane(CH4)uptake,nitrous oxide(N2O)emission,and global warming potential(GWP)was 0.70(95%Confidence Interval(CI):0.21–1.19),-0.27(95%CI:-0.72–0.18),and-0.39(95%CI:-1.01–0.23),respectively.In temperate climate zones with alkaline soils,the nitrogen application rate of 120–240 kg/ha,NT could significantly reduce GHG emissions and GWP.However,the mitigation effect will be weakened along with NT duration,except for proper straw addition.Overall,NT has the potential to reduce GHG emissions from wheat-based rotation systems in China,but it is necessary to implement NT depending on local conditions,soil characteristics,and field management.
