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miRNA-34b/c-5p对乳腺癌中神经激肽1受体-截短型表达的影响 被引量:2
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作者 张露方 王璐珊 +1 位作者 董冬 周云丽 《中国肿瘤临床》 CAS CSCD 北大核心 2018年第7期345-349,共5页
目的:探究乳腺癌中miRNA-34b/c-5p对神经激肽1受体-截短型(neurokinin1 receptor-truncated,NK1R-Tr)表达的影响及miRNA-34b/c-5p和NK1R-Tr对乳腺癌细胞迁移侵袭能力的影响。方法:收集2013年2月至5月天津医科大学肿瘤医院50例乳腺癌患... 目的:探究乳腺癌中miRNA-34b/c-5p对神经激肽1受体-截短型(neurokinin1 receptor-truncated,NK1R-Tr)表达的影响及miRNA-34b/c-5p和NK1R-Tr对乳腺癌细胞迁移侵袭能力的影响。方法:收集2013年2月至5月天津医科大学肿瘤医院50例乳腺癌患者组织标本,采用实时荧光定量PCR(RT-PCR)检测miRNA-34b/c-5p及NK1R-Tr的表达,采用蛋白免疫印迹实验检测乳腺癌MDA-MB-231和MCF-7细胞中miRNA-34b/c-5p对NK1R-Tr表达的影响,采用划痕及Transwell实验检测miRNA-34b/c-5p及NK1R-Tr对MDA-MB-231细胞迁移及侵袭能力的影响。结果:在乳腺癌组织及MDA-MB-231和MCF-7细胞中,miRNA-34b-5p和miRNA-34c-5p与NK1R-Tr表达呈负相关。发生淋巴结转移的乳腺癌患者组织中NK1R-Tr的相对表达量为5.75,未发生淋巴结转移者相对表达量为4.29,两者比较差异具有统计学意义(P=0.026)。过表达miRNA-34b/c-5p及敲低NK1R-Tr可以显著抑制MDA-MB-231细胞的迁移侵袭能力(均P<0.001)。结论:乳腺癌中miRNA-34b/c-5p与NK1R-Tr表达呈负相关,且miRNA-34b/c-5p和NK1R-Tr可能成为抑制乳腺癌转移的潜在治疗靶点。 展开更多
关键词 神经激肽1受体 MIRNA 乳腺癌 迁移 侵袭
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A review of the interaction between diet composition and gut microbiota and its impact on associated disease 被引量:1
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作者 Zhaoxi Liu Meihua Liu +2 位作者 Jing Meng lushan wang Min Chen 《Journal of Future Foods》 2024年第3期221-232,共12页
Dietary intake has an impact on the development of gut microbiota.Humans require carbohydrates,protein,fat,and other nutrients on a daily basis to provide energy for the growth,maintenance,and repair of body tissues.T... Dietary intake has an impact on the development of gut microbiota.Humans require carbohydrates,protein,fat,and other nutrients on a daily basis to provide energy for the growth,maintenance,and repair of body tissues.These nutrition-induced changes in gut microbiota may be used to alter host physiology,including disease development and progression,such as obesity and diabetes.More research is needed to fully understand how diet influences microbiota and how microbiota influence host health.The pathways of carbohydrate,protein,and fat metabolism,as well as their interactions and regulatory mechanisms,are described in this review,as well as how diet shapes the microbiota,how dietary-microbiome crosstalk may affect disease development and progression,and how this information could be used to maintain intestinal health. 展开更多
关键词 Gut microbiota Carbohydrates Amino acids Short-chain fatty acids PREBIOTICS
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肠道菌群多糖利用及代谢 被引量:12
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作者 刘昭曦 王禄山 陈敏 《微生物学报》 CAS CSCD 北大核心 2021年第7期1816-1828,共13页
宿主与肠道共生菌之间存在一种互利共生的关系。肠道共生菌可以代谢宿主自身不能消化的多糖。进入肠道内的多糖是影响肠道共生菌生理状态和组成的重要因素,这些多糖主要来自饮食和宿主的粘膜分泌物。人类饮食中含有几十种不同的膳食多糖... 宿主与肠道共生菌之间存在一种互利共生的关系。肠道共生菌可以代谢宿主自身不能消化的多糖。进入肠道内的多糖是影响肠道共生菌生理状态和组成的重要因素,这些多糖主要来自饮食和宿主的粘膜分泌物。人类饮食中含有几十种不同的膳食多糖,其中大多数不能被人类基因组中编码的酶降解,并进入大肠,供肠道共生菌利用。肠道共生菌将这些不易消化的多糖转化为短链脂肪酸,作为大肠细胞和其他肠道上皮细胞的营养物质。除此之外,短链脂肪酸对人体健康有着重要的影响。不同的肠道共生菌对进入肠道内的多糖具有不同的偏好性,表明摄入膳食多糖是一种可以直接影响肠道内共生菌物种平衡的策略。因此,研究肠道菌群的多糖代谢机制具有重要的意义。本文从肠道共生菌的组成、利用进入肠道内多糖的机制,以及产生的代谢产物可能对人体健康存在的潜在影响等方面进行了综述,并介绍了代表性的肠道共生菌如拟杆菌和双歧杆菌利用多糖的途径及特征。 展开更多
关键词 肠道菌群 多糖 膳食纤维 短链脂肪酸
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Secretome profiling reveals temperature-dependent growth of Aspergillus fumigatus 被引量:2
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作者 Dongyu wang Lili Zhang +1 位作者 Haiyue Zou lushan wang 《Science China(Life Sciences)》 SCIE CAS CSCD 2018年第5期578-592,共15页
Aspergillus fumigatus is a ubiquitous opportunistic fungus. In this study, systematic analyses were carried out to study the temperature adaptability of A. fumigatus. A total of 241 glycoside hydrolases and 69 proteas... Aspergillus fumigatus is a ubiquitous opportunistic fungus. In this study, systematic analyses were carried out to study the temperature adaptability of A. fumigatus. A total of 241 glycoside hydrolases and 69 proteases in the secretome revealed the strong capability of A. fumigatus to degrade plant biomass and protein substrates. In total, 129 pathogenesis-related proteins detected in the secretome were strongly correlated with glycoside hydrolases and proteases. The variety and abundance of proteins remained at temperatures of 34°C–45°C. The percentage of endo-1,4-xylanase increased when the temperature was lowered to 20°C, while the percentage of cellobiohydrolase increased as temperature was increased, suggesting that the strain obtains carbon mainly by degrading xylan and cellulose, and the main types of proteases in the secretome were aminopeptidases and carboxypeptidases. Only half of the proteins were retained and their abundance declined to 9.7% at 55°C. The activities of the remaining β-glycosidases and proteases were merely 35% and 24%, respectively, when the secretome was treated at 60°C for 2 h. Therefore, temperatures >60°C restrict the growth of A. fumigatus. 展开更多
关键词 Aspergillus fumigatus functional secretome temperature adaptability opportunistic pathogen saprophytic fungus
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