Tetrastigma hemsleyanum(T.hemsleyanum)has been regarded as an anticancer food in China.However,its corresponding mechanisms remains unclear.Thus,in this study,the antitumor activity of flavones-rich fraction of root o...Tetrastigma hemsleyanum(T.hemsleyanum)has been regarded as an anticancer food in China.However,its corresponding mechanisms remains unclear.Thus,in this study,the antitumor activity of flavones-rich fraction of root of T.hemsleyanum(FRTH)was investigated in vitro and in vivo.The results indicated that FRTH could inhibit the proliferation and migration of human hepatoellular carcinomas(HepG2)cells in vitro by the phosphatidylinositol 3-kinase/protein kinase B(PI3K/AKT)pathway.FRTH could increase the level of reactive oxygen species and change the mitochondrial membrane potential in HepG2 cells.In addition,FRTH treatment(300 mg/kg and 600 mg/kg,body weight)significantly suppressed tumor growth on HepG2 tumor-bearing nude mice.Besides,immunohistochemistry assays and western blotting revealed that FRTH enhanced the expression level of Bcl-2 associated X protein/B-cell lymphoma-2(Bax/Bcl-2),cytochrome C,caspase-3,caspase-9,and cleaved-caspase-3,and downregulated the expression level of platelet endothelial cell adhesion molecule-1(CD31),ki67,and vascular endothelial growth factor(VEGF)factor in HepG2 tumor-bearing mice.Our study suggests T.hemsleyanum as a kind of promising candidate medicine for liver cancer treatment.展开更多
Type 2 diabetes(T2D)is a chronic metabolic disease related to glucose metabolism disorders.Increasing evidences indicate that flavonoids obtained from plants have beneficial effects on T2D.Apios americana Medik,origin...Type 2 diabetes(T2D)is a chronic metabolic disease related to glucose metabolism disorders.Increasing evidences indicate that flavonoids obtained from plants have beneficial effects on T2D.Apios americana Medik,originally cultivated in North America,is rich in flavonoids.In this study,A.americana flower extract(AFE)was purified and flavonoids were identified.The beneficial effects of AFE on glucose metabolism disorders were investigated.Results showed that AFE protected hepatic cells from oxidative stress caused by hyperglycemia and increased glucose consumption and glucose uptake.The AKT pathway was involved in AFE-mediated hypoglycemic action in LO2 cells,accompanied by phosphorylation of FoxO1,Gsk-3βand AMPK.In vivo,AFE protected Caenorhabditis elegans(C.elegans)from oxidative stress under hyperglycemia and restored the expressions of PMK-1 and SKN-1.Furthermore,AFE decreased excessive glucose and lipid accumulation and extended the lifespans of high glucose(HG)treated C.elegans.AFE might be beneficial to decrease glucose metabolism disorders and T2D.展开更多
基金This research was based on the‘Functional food research and development of Tetrastigma hemsleyanum’funded by the Shanghai Zhengyue Enterprise Management Co.,Ltd.and the Science and Technology Department of Zhejiang Province(No.2018C02045),China.
文摘Tetrastigma hemsleyanum(T.hemsleyanum)has been regarded as an anticancer food in China.However,its corresponding mechanisms remains unclear.Thus,in this study,the antitumor activity of flavones-rich fraction of root of T.hemsleyanum(FRTH)was investigated in vitro and in vivo.The results indicated that FRTH could inhibit the proliferation and migration of human hepatoellular carcinomas(HepG2)cells in vitro by the phosphatidylinositol 3-kinase/protein kinase B(PI3K/AKT)pathway.FRTH could increase the level of reactive oxygen species and change the mitochondrial membrane potential in HepG2 cells.In addition,FRTH treatment(300 mg/kg and 600 mg/kg,body weight)significantly suppressed tumor growth on HepG2 tumor-bearing nude mice.Besides,immunohistochemistry assays and western blotting revealed that FRTH enhanced the expression level of Bcl-2 associated X protein/B-cell lymphoma-2(Bax/Bcl-2),cytochrome C,caspase-3,caspase-9,and cleaved-caspase-3,and downregulated the expression level of platelet endothelial cell adhesion molecule-1(CD31),ki67,and vascular endothelial growth factor(VEGF)factor in HepG2 tumor-bearing mice.Our study suggests T.hemsleyanum as a kind of promising candidate medicine for liver cancer treatment.
基金supported by the Zhejiang Provincial Major R&D Program[2021C02020]the Zhejiang Provincial Natural Science Foundation of China[LQ20C160011].
文摘Type 2 diabetes(T2D)is a chronic metabolic disease related to glucose metabolism disorders.Increasing evidences indicate that flavonoids obtained from plants have beneficial effects on T2D.Apios americana Medik,originally cultivated in North America,is rich in flavonoids.In this study,A.americana flower extract(AFE)was purified and flavonoids were identified.The beneficial effects of AFE on glucose metabolism disorders were investigated.Results showed that AFE protected hepatic cells from oxidative stress caused by hyperglycemia and increased glucose consumption and glucose uptake.The AKT pathway was involved in AFE-mediated hypoglycemic action in LO2 cells,accompanied by phosphorylation of FoxO1,Gsk-3βand AMPK.In vivo,AFE protected Caenorhabditis elegans(C.elegans)from oxidative stress under hyperglycemia and restored the expressions of PMK-1 and SKN-1.Furthermore,AFE decreased excessive glucose and lipid accumulation and extended the lifespans of high glucose(HG)treated C.elegans.AFE might be beneficial to decrease glucose metabolism disorders and T2D.
