The visualization of drugs in living systems has become key techniques in modern therapeutics.Recent advancements in optical imaging technologies and molecular design strategies have revolutionized drug visualization....The visualization of drugs in living systems has become key techniques in modern therapeutics.Recent advancements in optical imaging technologies and molecular design strategies have revolutionized drug visualization.At the subcellular level,super-resolution microscopy has allowed exploration of the molecular landscape within individual cells and the cellular response to drugs.Moving beyond subcellular imaging,researchers have integrated multiple modes,like optical near-infrared II imaging,to study the complex spatiotemporal interactions between drugs and their surroundings.By combining these visualization approaches,researchers gain supplementary information on physiological parameters,metabolic activity,and tissue composition,leading to a comprehensive understanding of drug behavior.This review focuses on cutting-edge technologies in drug visualization,particularly fluorescence imaging,and the main types of fluorescent molecules used.Additionally,we discuss current challenges and prospects in targeted drug research,emphasizing the importance of multidisciplinary cooperation in advancing drug visualization.With the integration of advanced imaging technology and molecular design,drug visualization has the potential to redefine our understanding of pharmacology,enabling the analysis of drug micro-dynamics in subcellular environments from new perspectives and deepening pharmacological research to the levels of the cell and organelles.展开更多
基金supported by the Shandong Province Key R&D Program(Major Technological Innovation Project,2021CXGC010501,China)National Natural Science Foundation of China(Nos.22107059,22007060,32300957,82141209)+9 种基金Young Elite Scientists Sponsorship Program by CACM,China(CACM-2023-QNRC1-02)the key Program of Natural Science Foundation of Shandong Province(ZR2023ZD25,China)Natural Science Foundation of Shandong Province(ZR2021QH057,ZR2022QH304,ZR2020QB166,ZR2023QH427,China)Innovation Team of Shandong Higher School Youth Innovation Technology Program(2021KJ035,2022KJ197,China)Taishan Scholars Project in Shandong Province,China(TSPD20181218 TSTP20230633 TSQN202211221)Shandong Science Fund for Excellent Young Scholars(ZR2022YQ66,China)Jinan New 20 Policies for Higher Education Funding(202228048,China)Natural Science Foundation of Shandong Province(Joint Fundation for Innovation and Development,ZR2022LZY021,China)Youth Qihuang Scholars Support Program of the State Administration of Traditional Chinese Medicine,Tianjin Graduate Research Innovation Project(General Project,2022BKY180,China)TUTCM Graduate Research Innovation Project(General Project)and Shandong Province Traditional Chinese Medicine Science and Technology Project(M-2023208,China).
文摘The visualization of drugs in living systems has become key techniques in modern therapeutics.Recent advancements in optical imaging technologies and molecular design strategies have revolutionized drug visualization.At the subcellular level,super-resolution microscopy has allowed exploration of the molecular landscape within individual cells and the cellular response to drugs.Moving beyond subcellular imaging,researchers have integrated multiple modes,like optical near-infrared II imaging,to study the complex spatiotemporal interactions between drugs and their surroundings.By combining these visualization approaches,researchers gain supplementary information on physiological parameters,metabolic activity,and tissue composition,leading to a comprehensive understanding of drug behavior.This review focuses on cutting-edge technologies in drug visualization,particularly fluorescence imaging,and the main types of fluorescent molecules used.Additionally,we discuss current challenges and prospects in targeted drug research,emphasizing the importance of multidisciplinary cooperation in advancing drug visualization.With the integration of advanced imaging technology and molecular design,drug visualization has the potential to redefine our understanding of pharmacology,enabling the analysis of drug micro-dynamics in subcellular environments from new perspectives and deepening pharmacological research to the levels of the cell and organelles.